Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1387(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1387
Peptide sequence	FPQY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Phe-Pro-Gln-Tyr

Single-letter amino acid

FPQY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	553.61 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

300.74 ± 69.09 μM

pIC₅₀

-2.478

GRAVY

-0.9000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	α_s2-Casein
Residue position	f(92-95)
Precursor protein(s) search	Source.1: DFBPPR1420 ---- Plant proteins ---- Protein HEADING DATE 3B Source.2: DFBPPR2217 ---- Plant proteins ---- Serine carboxypeptidase-like 26 Source.3: DFBPPR3826 ---- Plant proteins ---- Profilin LP04 Source.4: DFBPPR4383 ---- Plant proteins ---- ELF3-like protein 2 Source.5: DFBPPR7166 ---- Plant proteins ---- Serine carboxypeptidase II-2 Source.6: DFBPPR7182 ---- Plant proteins ---- Serine carboxypeptidase II-1 Source.7: DFBPPR7679 ---- Milk proteins ---- Alpha-S2-casein Source.8: DFBPPR7689 ---- Milk proteins ---- Alpha-S2-casein Source.9: DFBPPR7701 ---- Milk proteins ---- Alpha-S2-casein Source.10: DFBPPR8494 ---- Milk proteins ---- Alpha-S2-casein Source.11: DFBPPR16031 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit Source.12: DFBPPR19476 ---- Animal proteins ---- Rho GTPase-activating protein 7 Source.13: DFBPPR20710 ---- Animal proteins ---- BMP/retinoic acid-inducible neural-specific protein 1 Source.14: DFBPPR21159 ---- Animal proteins ---- Origin recognition complex subunit 4 Source.15: DFBPPR8815 ---- Animal proteins ---- Adenylyltransferase and sulfurtransferase MOCS3 Source.16: DFBPPR10689 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 13 Source.17: DFBPPR10832 ---- Animal proteins ---- BMP/retinoic acid-inducible neural-specific protein 1 Source.18: DFBPPR11854 ---- Animal proteins ---- Claw keratin Source.19: DFBPPR13045 ---- Animal proteins ---- Alpha-S2-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 300.74 ± 69.09 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide was obtained from the peptide YQKFPQY after the action of digestive enzymes including pepsin A (E.C. 3.4.23.1) and Corolase PP.

Stability & Cytotoxicity

Peptide stability

Literature report:	Peptide FPQY has resistant to gastrointestinal digestive enzymes including pepsin A (E.C. 3.4.23.1) and Corolase PP.
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX0677
[D2]	DFBPMUFU0353

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Contreras, M.d.M., Sanchez, D., Sevilla, M.Á., Recio, I., Amigo, L. Resistance of casein-derived bioactive peptides to simulated gastrointestinal digestion. International Dairy Journal. 2013, 32, 71-8. DOI: 10.1016/j.idairyj.2013.05.008
Other literature(s)	N.D
PubDate	2013