Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1478(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1478
Peptide sequence	LHLPLP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Leu-His-Leu-Pro-Leu-Pro

Single-letter amino acid

LHLPLP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	688.85 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

2.9 μM

pIC₅₀

-0.462

GRAVY

0.8333^c

Hydrophilic residue ratio

83.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(124-129)
Precursor protein(s) search	Source.1: DFBPPR3187 ---- Plant proteins ---- Probable glucuronosyltransferase Os10g0205300 Source.2: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.3: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.5: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.6: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.7: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.8: DFBPPR16270 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.9: DFBPPR19085 ---- Animal proteins ---- AP-4 complex subunit mu-1
Link-research Inductive analysis	Link 1: DFBPACEI0420----Bovine milk protein----β-Casein

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 2.9 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	The peptides used in this study were synthesized by the solid-phase method.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANHY0002
[D2]	DFBPMUFU0117

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Kohmura, M., Nio, N., Kubo, K., Minoshima, Y., Munekata, E., Ariyoshi, Y. Inhibition of Angiotensin-converting Enzyme by Synthetic Peptides of Human β-Casein. Agricultural and Biological Chemistry. 2014, 53, 2107-14. DOI: 10.1080/00021369.1989.10869621
Other literature(s)	N.D
PubDate	2014