Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1528(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1528
Peptide sequence	IVGRPR
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ile-Val-Gly-Arg-Pro-Arg

Single-letter amino acid

IVGRPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	696.84 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

12.50 ^c

IC₅₀

300 uM

pIC₅₀

-2.477

GRAVY

-0.3833^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine, Fish
Organism/Source	Dried bonito (Katsuobusi)
Precursor protein	Muscle protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2751 ---- Plant proteins ---- Actin-7 Source.2: DFBPPR2763 ---- Plant proteins ---- Actin-1 Source.3: DFBPPR2989 ---- Plant proteins ---- Actin-2 Source.4: DFBPPR3087 ---- Plant proteins ---- Actin-3 Source.5: DFBPPR5185 ---- Plant proteins ---- Actin-1 Source.6: DFBPPR5283 ---- Plant proteins ---- Actin-3 Source.7: DFBPPR5841 ---- Plant proteins ---- Actin-1 Source.8: DFBPPR6550 ---- Plant proteins ---- Actin-3 Source.9: DFBPPR6551 ---- Plant proteins ---- Actin-2 Source.10: DFBPPR6552 ---- Plant proteins ---- Actin-1 Source.11: DFBPPR16188 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.12: DFBPPR16883 ---- Animal proteins ---- Actin, aortic smooth muscle Source.13: DFBPPR17154 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.14: DFBPPR17155 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.15: DFBPPR17514 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.16: DFBPPR17747 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.17: DFBPPR18495 ---- Animal proteins ---- Actin, gamma-enteric smooth muscle Source.18: DFBPPR8884 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.19: DFBPPR10103 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.20: DFBPPR10319 ---- Animal proteins ---- Actin, alpha cardiac muscle 1 Source.21: DFBPPR10338 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.22: DFBPPR10431 ---- Animal proteins ---- Actin, aortic smooth muscle Source.23: DFBPPR10478 ---- Animal proteins ---- Actin, gamma-enteric smooth muscle Source.24: DFBPPR10777 ---- Animal proteins ---- Actin, cytoplasmic type 5 Source.25: DFBPPR10810 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.26: DFBPPR12268 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.27: DFBPPR12556 ---- Animal proteins ---- Actin, aortic smooth muscle Source.28: DFBPPR12700 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.29: DFBPPR12701 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.30: DFBPPR13231 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.31: DFBPPR13769 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.32: DFBPPR13996 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.33: DFBPPR14021 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.34: DFBPPR14143 ---- Marine protein ---- Actin, cytoplasmic 1 Source.35: DFBPPR14935 ---- Microorganism protein ---- Actin Source.36: DFBPPR7844 ---- Plant protein ---- Actin-1
Link-research Inductive analysis	Link 1: DFBPACEI1008----Skate (Rajiformes: Rajidae)----Thornback ray muscle (Raja clavata)

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited moderate Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 300 uM (210 μg/mL).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Peptides were synthesized by a solid phase method with a peptide synthesizer (Biosearch SAM TWO).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANHY0607
[D2]	DFBPMUFU0420

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Yokoyama K, Chiba H, Yoshikawa M. Peptide inhibitors for angiotensin I-converting enzyme from thermolysin digest of dried bonito. Biosci Biotechnol Biochem. 1992 Oct;56(10):1541-5. PMID: 1369054 DOI: 10.1271/bbb.56.1541
Other literature(s)	N.D
PubDate	1992