Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1538(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1538
Peptide sequence	GLYP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Leu-Tyr-Pro

Single-letter amino acid

GLYP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	448.51 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

190 uM

pIC₅₀

-2.279

GRAVY

0.1250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine, Fish
Organism/Source	Dried bonito (Katsuobusi)
Precursor protein	Muscle protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1114 ---- Plant proteins ---- Pyruvate kinase 1, cytosolic Source.2: DFBPPR1222 ---- Plant proteins ---- Protein CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR 1 Source.3: DFBPPR1453 ---- Plant proteins ---- Crossover junction endonuclease MUS81 Source.4: DFBPPR2001 ---- Plant proteins ---- Importin subunit alpha-1b Source.5: DFBPPR2084 ---- Plant proteins ---- Pyruvate kinase 2, cytosolic Source.6: DFBPPR2419 ---- Plant proteins ---- U-box domain-containing protein 73 Source.7: DFBPPR2484 ---- Plant proteins ---- Translation factor GUF1 homolog, mitochondrial Source.8: DFBPPR2753 ---- Plant proteins ---- Transportin-1 Source.9: DFBPPR2979 ---- Plant proteins ---- Mitochondrial outer membrane protein porin 1 Source.10: DFBPPR3566 ---- Plant proteins ---- Probable protein phosphatase 2C 65 Source.11: DFBPPR5478 ---- Plant proteins ---- 1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic Source.12: DFBPPR6008 ---- Plant proteins ---- Zein-beta Source.13: DFBPPR6071 ---- Plant proteins ---- Photosystem I assembly protein Ycf4 Source.14: DFBPPR6227 ---- Plant proteins ---- Gibberellin 3-beta-dioxygenase 1 Source.15: DFBPPR6950 ---- Plant proteins ---- Photosystem I assembly protein Ycf4 Source.16: DFBPPR7316 ---- Plant proteins ---- Photosystem I assembly protein Ycf4 Source.17: DFBPPR7620 ---- Milk proteins ---- Polymeric immunoglobulin receptor Source.18: DFBPPR15975 ---- Animal proteins ---- Paired box protein Pax-8 Source.19: DFBPPR16201 ---- Animal proteins ---- X-linked retinitis pigmentosa GTPase regulator Source.20: DFBPPR16334 ---- Animal proteins ---- Alpha-(1,6)-fucosyltransferase Source.21: DFBPPR17387 ---- Animal proteins ---- ATP-dependent DNA/RNA helicase DHX36 Source.22: DFBPPR17436 ---- Animal proteins ---- Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 Source.23: DFBPPR17578 ---- Animal proteins ---- Acidic mammalian chitinase Source.24: DFBPPR17854 ---- Animal proteins ---- Tyrosine 3-monooxygenase Source.25: DFBPPR18142 ---- Animal proteins ---- Protein CASC3 Source.26: DFBPPR18151 ---- Animal proteins ---- Coagulation factor XIII A chain Source.27: DFBPPR18160 ---- Animal proteins ---- Protein kinase C and casein kinase substrate in neurons protein 1 Source.28: DFBPPR18499 ---- Animal proteins ---- Alpha-(1,6)-fucosyltransferase Source.29: DFBPPR18554 ---- Animal proteins ---- Arylsulfatase A Source.30: DFBPPR18598 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit beta-1 Source.31: DFBPPR18608 ---- Animal proteins ---- ATP-dependent RNA helicase DHX30 Source.32: DFBPPR19195 ---- Animal proteins ---- V-type proton ATPase 116 kDa subunit a2 Source.33: DFBPPR19597 ---- Animal proteins ---- Protein HEXIM1 Source.34: DFBPPR19861 ---- Animal proteins ---- Phospholipid phosphatase-related protein type 2 Source.35: DFBPPR19987 ---- Animal proteins ---- SH3 and cysteine-rich domain-containing protein Source.36: DFBPPR20490 ---- Animal proteins ---- Ornithine aminotransferase, mitochondrial Source.37: DFBPPR20562 ---- Animal proteins ---- 12S rRNA N4-methylcytidine methyltransferase Source.38: DFBPPR20602 ---- Animal proteins ---- Interferon regulatory factor 6 Source.39: DFBPPR20673 ---- Animal proteins ---- Trafficking protein particle complex subunit 9 Source.40: DFBPPR21195 ---- Animal proteins ---- Vesicular, overexpressed in cancer, prosurvival protein 1 Source.41: DFBPPR21255 ---- Animal proteins ---- Homeobox protein Hox-B7 Source.42: DFBPPR21728 ---- Animal proteins ---- Galectin-9 Source.43: DFBPPR22044 ---- Animal proteins ---- Transgelin-2 Source.44: DFBPPR22060 ---- Animal proteins ---- Transmembrane protein 126A Source.45: DFBPPR22241 ---- Animal proteins ---- von Willebrand factor A domain-containing protein 7 Source.46: DFBPPR22271 ---- Animal proteins ---- Primary cilium assembly protein FAM149B1 Source.47: DFBPPR22316 ---- Animal proteins ---- MAPK-interacting and spindle-stabilizing protein-like Source.48: DFBPPR22710 ---- Animal proteins ---- RIIa domain-containing protein 1 Source.49: DFBPPR8594 ---- Animal proteins ---- Trifunctional enzyme subunit alpha, mitochondrial Source.50: DFBPPR8856 ---- Animal proteins ---- Alpha-(1,6)-fucosyltransferase Source.51: DFBPPR8981 ---- Animal proteins ---- Deleted in malignant brain tumors 1 protein Source.52: DFBPPR8992 ---- Animal proteins ---- Hyaluronidase-3 Source.53: DFBPPR9031 ---- Animal proteins ---- Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA Source.54: DFBPPR10227 ---- Animal proteins ---- Serine/threonine-protein kinase STK11 Source.55: DFBPPR10312 ---- Animal proteins ---- Protein kinase C and casein kinase substrate in neurons protein 2 Source.56: DFBPPR11008 ---- Animal proteins ---- Homeobox protein NANOG Source.57: DFBPPR11201 ---- Animal proteins ---- ATP-dependent RNA helicase DHX30 Source.58: DFBPPR11322 ---- Animal proteins ---- Homeobox protein Hox-D4 Source.59: DFBPPR11431 ---- Animal proteins ---- Putative glycerol kinase 5 Source.60: DFBPPR11968 ---- Animal proteins ---- AT-rich interactive domain-containing protein 5B Source.61: DFBPPR12546 ---- Animal proteins ---- Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA Source.62: DFBPPR13262 ---- Animal proteins ---- Gastrin Source.63: DFBPPR13749 ---- Animal proteins ---- Uroporphyrinogen decarboxylase Source.64: DFBPPR14553 ---- Marine protein ---- Glucocorticoid receptor Source.65: DFBPPR15314 ---- Microorganism protein ---- Probable metalloprotease ARX1 Source.66: DFBPPR15409 ---- Microorganism protein ---- Mitochondrial inner membrane magnesium transporter MRS2 Source.67: DFBPPR15885 ---- Microorganism protein ---- RNA-directed RNA polymerase Source.68: DFBPPR7791 ---- Plant protein ---- Translation factor GUF1 homolog, mitochondrial Source.69: DFBPPR7905 ---- Plant protein ---- Photosystem I assembly protein Ycf4
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 190 uM (85 μg/mL).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Peptides were synthesized by a solid phase method with a peptide synthesizer (Biosearch SAM TWO).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yokoyama K, Chiba H, Yoshikawa M. Peptide inhibitors for angiotensin I-converting enzyme from thermolysin digest of dried bonito. Biosci Biotechnol Biochem. 1992 Oct;56(10):1541-5. PMID: 1369054 DOI: 10.1271/bbb.56.1541
Other literature(s)	N.D
PubDate	1992