Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1570(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1570
Peptide sequence	LPPPVH
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	PEP-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Leu-Pro-Pro-Pro-Val-His

Single-letter amino acid

LPPPVH

Peptide length

Peptide mass

Experimental mass	Theoretical mass
659 Da	658.79 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

> 1000 μM

pIC₅₀

> -3.0

GRAVY

-0.0000^c

Hydrophilic residue ratio

83.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Maize endosperm
Precursor protein	γ-Zein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR5706 ---- Plant proteins ---- Glutelin-2 Source.2: DFBPPR16647 ---- Animal proteins ---- Tudor domain-containing protein 7 Source.3: DFBPPR20302 ---- Animal proteins ---- General transcription factor IIH subunit 3 Source.4: DFBPPR20981 ---- Animal proteins ---- Tudor domain-containing protein 7
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide showed almost no Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of > 1000 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	The peptide was synthesized by the solid phase method with a 430A Peptide Synthesizer (Applied Biosystems, Inc.).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPPEPI0014
[D2]	DFBPMUFU0435

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Maruyama, S., Miyoshi, S., Kaneko, T., Tanaka, H. Angiotensin I-Converting Enzyme Inhibitory Activities of Synthetic Peptides Related to the Tandem Repeated Sequence of a Maize Endosperm Protein. Agricultural and Biological Chemistry. 2014, 53, 1077-81. DOI: 10.1080/00021369.1989.10869427
Other literature(s)	N.D
PubDate	2014