Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1576(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1576
Peptide sequence	AGSP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ala-Gly-Ser-Pro

Single-letter amino acid

AGSP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
331.2 Da	330.33 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

37.2 μM

pIC₅₀

-1.571

GRAVY

-0.2500^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine, Fish
Organism/Source	Cuttlefish (Sepia officinalis)
Precursor protein	Cuttlefish muscle protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0915 ---- Plant proteins ---- Kinesin-like protein KIN-4A Source.2: DFBPPR0951 ---- Plant proteins ---- Calpain-type cysteine protease ADL1 Source.3: DFBPPR0963 ---- Plant proteins ---- E3 ubiquitin-protein ligase CCNB1IP1 homolog Source.4: DFBPPR1034 ---- Plant proteins ---- bZIP transcription factor 50 Source.5: DFBPPR1255 ---- Plant proteins ---- Ethylene receptor 3 Source.6: DFBPPR1399 ---- Plant proteins ---- Phytoene synthase 3, chloroplastic Source.7: DFBPPR1419 ---- Plant proteins ---- Gibberellin 3-beta-dioxygenase 1 Source.8: DFBPPR1425 ---- Plant proteins ---- Transcription factor BHLH156 Source.9: DFBPPR1508 ---- Plant proteins ---- Gamma-aminobutyrate transaminase 1, mitochondrial Source.10: DFBPPR1518 ---- Plant proteins ---- GATA transcription factor 15 Source.11: DFBPPR1544 ---- Plant proteins ---- Protein disulfide isomerase-like 2-3 Source.12: DFBPPR1593 ---- Plant proteins ---- WUSCHEL-related homeobox 11 Source.13: DFBPPR1822 ---- Plant proteins ---- Probable E3 ubiquitin-protein ligase HIP1 Source.14: DFBPPR1862 ---- Plant proteins ---- Heat stress transcription factor B-2c Source.15: DFBPPR1917 ---- Plant proteins ---- Kinesin-like protein KIN-12A Source.16: DFBPPR1921 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX7 Source.17: DFBPPR1987 ---- Plant proteins ---- Probable gamma-aminobutyrate transaminase 4 Source.18: DFBPPR2002 ---- Plant proteins ---- bZIP transcription factor 60 Source.19: DFBPPR2013 ---- Plant proteins ---- Laccase-6 Source.20: DFBPPR2176 ---- Plant proteins ---- Expansin-B11 Source.21: DFBPPR2278 ---- Plant proteins ---- Zinc finger protein CO3 Source.22: DFBPPR2353 ---- Plant proteins ---- Expansin-B12 Source.23: DFBPPR2382 ---- Plant proteins ---- Putative cellulose synthase-like protein H3 Source.24: DFBPPR2535 ---- Plant proteins ---- Probable glucuronosyltransferase Os04g0650300 Source.25: DFBPPR2864 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 53 Source.26: DFBPPR2887 ---- Plant proteins ---- Probable protein phosphatase 2C 32 Source.27: DFBPPR2931 ---- Plant proteins ---- Putative expansin-B14 Source.28: DFBPPR3102 ---- Plant proteins ---- Expansin-B18 Source.29: DFBPPR3198 ---- Plant proteins ---- Probable protein phosphatase 2C 59 Source.30: DFBPPR3206 ---- Plant proteins ---- Expansin-B15 Source.31: DFBPPR3346 ---- Plant proteins ---- Peroxisomal membrane protein 11-2 Source.32: DFBPPR3352 ---- Plant proteins ---- Probable protein phosphatase 2C 68 Source.33: DFBPPR3362 ---- Plant proteins ---- Probable alpha-glucosidase Os06g0675700 Source.34: DFBPPR3475 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX14 Source.35: DFBPPR3529 ---- Plant proteins ---- Probable E3 ubiquitin-protein ligase XBOS36 Source.36: DFBPPR3545 ---- Plant proteins ---- Auxin response factor 3 Source.37: DFBPPR3859 ---- Plant proteins ---- Probable protein phosphatase 2C 29 Source.38: DFBPPR3862 ---- Plant proteins ---- Aquaporin NIP4-1 Source.39: DFBPPR4032 ---- Plant proteins ---- Cell division control protein 6 homolog Source.40: DFBPPR4171 ---- Plant proteins ---- 5'-adenylylsulfate reductase-like 4 Source.41: DFBPPR4308 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 28 Source.42: DFBPPR4377 ---- Plant proteins ---- Trafficking protein particle complex II-specific subunit 120 homolog Source.43: DFBPPR4392 ---- Plant proteins ---- WUSCHEL-related homeobox 7 Source.44: DFBPPR4428 ---- Plant proteins ---- Acyl transferase 9 Source.45: DFBPPR4565 ---- Plant proteins ---- CASP-like protein 5B1 Source.46: DFBPPR4633 ---- Plant proteins ---- B3 domain-containing protein Os07g0183700 Source.47: DFBPPR4650 ---- Plant proteins ---- BURP domain-containing protein 2 Source.48: DFBPPR4677 ---- Plant proteins ---- Putative protein Brevis radix-like 3 Source.49: DFBPPR4691 ---- Plant proteins ---- Probable protein ABIL3 Source.50: DFBPPR4695 ---- Plant proteins ---- SNW/SKI-interacting protein B Source.51: DFBPPR4758 ---- Plant proteins ---- BURP domain-containing protein 7 Source.52: DFBPPR4791 ---- Plant proteins ---- B3 domain-containing protein Os02g0683500 Source.53: DFBPPR4828 ---- Plant proteins ---- BURP domain-containing protein 6 Source.54: DFBPPR5279 ---- Plant proteins ---- Cytochrome P450 71D8 Source.55: DFBPPR5387 ---- Plant proteins ---- Calpain-type cysteine protease DEK1 Source.56: DFBPPR5631 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase 1 Source.57: DFBPPR5813 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase 2 Source.58: DFBPPR5832 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase 3 Source.59: DFBPPR6157 ---- Plant proteins ---- Ninja-family protein 5 Source.60: DFBPPR7053 ---- Plant proteins ---- Alpha-amylase/trypsin inhibitor CMa Source.61: DFBPPR7110 ---- Plant proteins ---- Glucan endo-1,3-beta-glucosidase GIII Source.62: DFBPPR7422 ---- Plant proteins ---- Napin-1A Source.63: DFBPPR7628 ---- Milk proteins ---- Complement C4-A Source.64: DFBPPR15942 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B Source.65: DFBPPR15990 ---- Animal proteins ---- Transcription factor SOX-9 Source.66: DFBPPR16027 ---- Animal proteins ---- Rho GTPase-activating protein 35 Source.67: DFBPPR16048 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.68: DFBPPR16266 ---- Animal proteins ---- Beta-1 adrenergic receptor Source.69: DFBPPR16456 ---- Animal proteins ---- Ribosome-binding protein 1 Source.70: DFBPPR16638 ---- Animal proteins ---- Synapsin-1 Source.71: DFBPPR16910 ---- Animal proteins ---- Casein kinase II subunit alpha Source.72: DFBPPR16934 ---- Animal proteins ---- Coagulation factor V Source.73: DFBPPR17162 ---- Animal proteins ---- Collagen alpha-1(IV) chain Source.74: DFBPPR17269 ---- Animal proteins ---- Phosphatidylinositol-glycan-specific phospholipase D Source.75: DFBPPR17353 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase-like N Source.76: DFBPPR17360 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.77: DFBPPR17410 ---- Animal proteins ---- Myotubularin-related protein 2 Source.78: DFBPPR17473 ---- Animal proteins ---- E3 ubiquitin-protein ligase TRIM71 Source.79: DFBPPR17802 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase FKBP4 Source.80: DFBPPR17854 ---- Animal proteins ---- Tyrosine 3-monooxygenase Source.81: DFBPPR17873 ---- Animal proteins ---- Rho family-interacting cell polarization regulator 2 Source.82: DFBPPR18200 ---- Animal proteins ---- RNA demethylase ALKBH5 Source.83: DFBPPR18265 ---- Animal proteins ---- D-3-phosphoglycerate dehydrogenase Source.84: DFBPPR18285 ---- Animal proteins ---- ADP-ribose glycohydrolase OARD1 Source.85: DFBPPR18299 ---- Animal proteins ---- Synapsin-1 Source.86: DFBPPR18318 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase BAP1 Source.87: DFBPPR18522 ---- Animal proteins ---- POU domain, class 5, transcription factor 1 Source.88: DFBPPR18537 ---- Animal proteins ---- Collagen alpha-1(III) chain Source.89: DFBPPR18865 ---- Animal proteins ---- Collagen alpha-1(XI) chain Source.90: DFBPPR18949 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase MINDY-2 Source.91: DFBPPR19033 ---- Animal proteins ---- Collagen alpha-2(XI) chain Source.92: DFBPPR19073 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 21 Source.93: DFBPPR19120 ---- Animal proteins ---- Collagen alpha-1(X) chain Source.94: DFBPPR19310 ---- Animal proteins ---- Chromatin assembly factor 1 subunit A Source.95: DFBPPR19357 ---- Animal proteins ---- Collagen alpha-2(IX) chain Source.96: DFBPPR19580 ---- Animal proteins ---- Endosomal/lysosomal potassium channel TMEM175 Source.97: DFBPPR19622 ---- Animal proteins ---- Thrombospondin type-1 domain-containing protein 1 Source.98: DFBPPR19660 ---- Animal proteins ---- Phosphorylase b kinase gamma catalytic chain, liver/testis isoform Source.99: DFBPPR19669 ---- Animal proteins ---- Cullin-associated NEDD8-dissociated protein 1 Source.100: DFBPPR19670 ---- Animal proteins ---- SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 Source.101: DFBPPR19768 ---- Animal proteins ---- Peroxynitrite isomerase THAP4 Source.102: DFBPPR19814 ---- Animal proteins ---- Protein delta homolog 2 Source.103: DFBPPR19838 ---- Animal proteins ---- Transcription factor GATA-5 Source.104: DFBPPR19861 ---- Animal proteins ---- Phospholipid phosphatase-related protein type 2 Source.105: DFBPPR19868 ---- Animal proteins ---- Inter-alpha-trypsin inhibitor heavy chain H1 Source.106: DFBPPR19943 ---- Animal proteins ---- Keratin, type II cytoskeletal 80 Source.107: DFBPPR20028 ---- Animal proteins ---- Sine oculis-binding protein homolog Source.108: DFBPPR20074 ---- Animal proteins ---- Target of EGR1 protein 1 Source.109: DFBPPR20167 ---- Animal proteins ---- Protein BANP Source.110: DFBPPR20254 ---- Animal proteins ---- Leukemia inhibitory factor Source.111: DFBPPR20301 ---- Animal proteins ---- Histidine triad nucleotide-binding protein 3 Source.112: DFBPPR20397 ---- Animal proteins ---- Medium-chain acyl-CoA ligase ACSF2, mitochondrial Source.113: DFBPPR20418 ---- Animal proteins ---- Alpha-1B-glycoprotein Source.114: DFBPPR20460 ---- Animal proteins ---- Monocarboxylate transporter 1 Source.115: DFBPPR20492 ---- Animal proteins ---- Microtubule-associated protein 10 Source.116: DFBPPR20609 ---- Animal proteins ---- Ran-binding protein 10 Source.117: DFBPPR20665 ---- Animal proteins ---- PWWP domain-containing DNA repair factor 3A Source.118: DFBPPR20701 ---- Animal proteins ---- Cysteine--tRNA ligase, mitochondrial Source.119: DFBPPR20980 ---- Animal proteins ---- Interferon-inducible GTPase 5 Source.120: DFBPPR21364 ---- Animal proteins ---- Rho guanine nucleotide exchange factor 10-like protein Source.121: DFBPPR21478 ---- Animal proteins ---- FTS and Hook-interacting protein Source.122: DFBPPR21679 ---- Animal proteins ---- Protein spinster homolog 1 Source.123: DFBPPR21715 ---- Animal proteins ---- Junctional protein associated with coronary artery disease homolog Source.124: DFBPPR21813 ---- Animal proteins ---- Ribosomal RNA processing protein 36 homolog Source.125: DFBPPR21837 ---- Animal proteins ---- Zinc finger protein 750 Source.126: DFBPPR22222 ---- Animal proteins ---- PGC-1 and ERR-induced regulator in muscle protein 1 Source.127: DFBPPR22609 ---- Animal proteins ---- Protein TSSC4 Source.128: DFBPPR8543 ---- Animal proteins ---- Sterol regulatory element-binding protein 1 Source.129: DFBPPR8578 ---- Animal proteins ---- Large proline-rich protein BAG6 Source.130: DFBPPR8609 ---- Animal proteins ---- Mothers against decapentaplegic homolog 3 Source.131: DFBPPR8748 ---- Animal proteins ---- Dual oxidase 2 Source.132: DFBPPR8905 ---- Animal proteins ---- Zonadhesin Source.133: DFBPPR8945 ---- Animal proteins ---- Coagulation factor V Source.134: DFBPPR9071 ---- Animal proteins ---- Nuclear receptor coactivator 1 Source.135: DFBPPR9153 ---- Animal proteins ---- D-3-phosphoglycerate dehydrogenase Source.136: DFBPPR9228 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1D Source.137: DFBPPR9310 ---- Animal proteins ---- Vasopressin V2 receptor Source.138: DFBPPR9538 ---- Animal proteins ---- Steroidogenic acute regulatory protein, mitochondrial Source.139: DFBPPR9580 ---- Animal proteins ---- Mediator of DNA damage checkpoint protein 1 Source.140: DFBPPR9738 ---- Animal proteins ---- Protein delta homolog 2 Source.141: DFBPPR9882 ---- Animal proteins ---- Krueppel-like factor 17 Source.142: DFBPPR9956 ---- Animal proteins ---- Fatty acid synthase Source.143: DFBPPR10056 ---- Animal proteins ---- Mothers against decapentaplegic homolog 3 Source.144: DFBPPR10068 ---- Animal proteins ---- Transcription factor SOX-9 Source.145: DFBPPR10196 ---- Animal proteins ---- Transcription factor Sp3 Source.146: DFBPPR10355 ---- Animal proteins ---- Down syndrome cell adhesion molecule-like protein 1 homolog Source.147: DFBPPR10470 ---- Animal proteins ---- Collagen alpha-1(III) chain Source.148: DFBPPR10479 ---- Animal proteins ---- Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Source.149: DFBPPR10520 ---- Animal proteins ---- Collagen alpha-2(IX) chain Source.150: DFBPPR10542 ---- Animal proteins ---- Erythroid transcription factor Source.151: DFBPPR10610 ---- Animal proteins ---- Denticleless protein homolog Source.152: DFBPPR10620 ---- Animal proteins ---- Centromere protein T Source.153: DFBPPR10729 ---- Animal proteins ---- Versican core protein Source.154: DFBPPR10730 ---- Animal proteins ---- Casein kinase II subunit alpha Source.155: DFBPPR10769 ---- Animal proteins ---- Ubiquitin thioesterase OTU1 Source.156: DFBPPR10786 ---- Animal proteins ---- Beta-1,3-N-acetylglucosaminyltransferase radical fringe Source.157: DFBPPR10820 ---- Animal proteins ---- Collagen alpha-1(IX) chain Source.158: DFBPPR10869 ---- Animal proteins ---- Ribosomal oxygenase 1 Source.159: DFBPPR10993 ---- Animal proteins ---- Pericentriolar material 1 protein Source.160: DFBPPR11053 ---- Animal proteins ---- Alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase Source.161: DFBPPR11182 ---- Animal proteins ---- Transcription factor HES-1 Source.162: DFBPPR11194 ---- Animal proteins ---- Steroidogenic acute regulatory protein, mitochondrial Source.163: DFBPPR11336 ---- Animal proteins ---- Monocarboxylate transporter 3 Source.164: DFBPPR11343 ---- Animal proteins ---- Transcription factor Sp8 Source.165: DFBPPR11347 ---- Animal proteins ---- Neogenin Source.166: DFBPPR11367 ---- Animal proteins ---- Insulin gene enhancer protein ISL-2 Source.167: DFBPPR11446 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 48 Source.168: DFBPPR11477 ---- Animal proteins ---- Transcription factor GATA-5 Source.169: DFBPPR11563 ---- Animal proteins ---- Switch-associated protein 70 Source.170: DFBPPR11724 ---- Animal proteins ---- Protein TENP Source.171: DFBPPR11812 ---- Animal proteins ---- Phosphorylated adapter RNA export protein Source.172: DFBPPR11943 ---- Animal proteins ---- Transcription initiation factor TFIID subunit 3 Source.173: DFBPPR11967 ---- Animal proteins ---- Monocarboxylate transporter 4 Source.174: DFBPPR12021 ---- Animal proteins ---- DNA repair protein RAD52 homolog Source.175: DFBPPR12079 ---- Animal proteins ---- Transcription factor SPT20 homolog Source.176: DFBPPR12272 ---- Animal proteins ---- Large neutral amino acids transporter small subunit 1 Source.177: DFBPPR12286 ---- Animal proteins ---- Helicase-like transcription factor Source.178: DFBPPR12294 ---- Animal proteins ---- Zona pellucida sperm-binding protein 3 Source.179: DFBPPR12317 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase FKBP4 Source.180: DFBPPR12351 ---- Animal proteins ---- 4F2 cell-surface antigen heavy chain Source.181: DFBPPR12370 ---- Animal proteins ---- Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform Source.182: DFBPPR12378 ---- Animal proteins ---- GDH/6PGL endoplasmic bifunctional protein Source.183: DFBPPR12411 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit epsilon Source.184: DFBPPR12432 ---- Animal proteins ---- Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Source.185: DFBPPR12473 ---- Animal proteins ---- Nitric oxide synthase, brain Source.186: DFBPPR12481 ---- Animal proteins ---- Casein kinase II subunit alpha Source.187: DFBPPR12570 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1D Source.188: DFBPPR12576 ---- Animal proteins ---- Chloride intracellular channel protein 6 Source.189: DFBPPR12901 ---- Animal proteins ---- Eukaryotic translation initiation factor 3 subunit I Source.190: DFBPPR13059 ---- Animal proteins ---- Protein Wnt-2 Source.191: DFBPPR13091 ---- Animal proteins ---- Cortactin-binding protein 2 Source.192: DFBPPR13346 ---- Animal proteins ---- Steroidogenic acute regulatory protein, mitochondrial Source.193: DFBPPR13822 ---- Animal proteins ---- Steroidogenic acute regulatory protein, mitochondrial Source.194: DFBPPR13947 ---- Animal proteins ---- Cortactin-binding protein 2 Source.195: DFBPPR14333 ---- Marine protein ---- Ferredoxin-dependent glutamate synthase Source.196: DFBPPR14812 ---- Marine protein ---- Alpha-2-macroglobulin homolog Source.197: DFBPPR15014 ---- Microorganism protein ---- AP-1-like transcription factor YAP1 Source.198: DFBPPR15143 ---- Microorganism protein ---- Low-affinity glucose transporter Source.199: DFBPPR15160 ---- Microorganism protein ---- Palmitoyltransferase PFA3 Source.200: DFBPPR15690 ---- Microorganism protein ---- Inclusion body clearance protein IML2 Source.201: DFBPPR15846 ---- Microorganism protein ---- Exoglucanase 3
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 37.2 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)NCC(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptides were isolated from the hydrolysate obtained by treatment with crude protease extract from Bacillus mojavensis A21.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Cuttlefish muscle is a promising protein source for the production of ACE inhibitory peptides that could be utilized to develop functional foods for prevention of hypertension.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Balti, R., Nedjar-Arroume, N., Adje, E.Y., Guillochon, D., Nasri, M. Analysis of novel angiotensin I-converting enzyme inhibitory peptides from enzymatic hydrolysates of cuttlefish (Sepia officinalis) muscle proteins. J Agric Food Chem. 2010, 58, 3840-6. DOI: 10.1021/jf904300q
Other literature(s)	N.D
PubDate	2010