Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1597(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1597
Peptide sequence	FYQQ
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Tyr-Gln-Gln

Single-letter amino acid

FYQQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	584.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

25 μM

pIC₅₀

-1.398

GRAVY

-1.3750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Maize protein
Precursor protein	α-Zein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1103 ---- Plant proteins ---- Villin-2 Source.2: DFBPPR1553 ---- Plant proteins ---- Transcription factor LG2 Source.3: DFBPPR2731 ---- Plant proteins ---- Eukaryotic translation initiation factor 4G Source.4: DFBPPR2856 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta Source.5: DFBPPR3481 ---- Plant proteins ---- PHD finger protein ALFIN-LIKE 8 Source.6: DFBPPR4399 ---- Plant proteins ---- IAA-amino acid hydrolase ILR1-like 5 Source.7: DFBPPR4938 ---- Plant proteins ---- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 2, mitochondrial Source.8: DFBPPR5591 ---- Plant proteins ---- Transcription factor LG2 Source.9: DFBPPR5797 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta Source.10: DFBPPR6029 ---- Plant proteins ---- Zein-alpha PMS2 Source.11: DFBPPR6037 ---- Plant proteins ---- 19 kDa alpha-zein 19C2 Source.12: DFBPPR6057 ---- Plant proteins ---- Zein-alpha PMS1 Source.13: DFBPPR6080 ---- Plant proteins ---- Zein-alpha 19A2 Source.14: DFBPPR6081 ---- Plant proteins ---- Zein-alpha 19B1 Source.15: DFBPPR6085 ---- Plant proteins ---- Zein-alpha A30 Source.16: DFBPPR6088 ---- Plant proteins ---- Zein-alpha ZG99 Source.17: DFBPPR6093 ---- Plant proteins ---- Zein-alpha 19C1 Source.18: DFBPPR6095 ---- Plant proteins ---- Zein-alpha A20 Source.19: DFBPPR6096 ---- Plant proteins ---- Zein-alpha GZ19AB11 Source.20: DFBPPR6101 ---- Plant proteins ---- Zein-alpha M6 Source.21: DFBPPR6110 ---- Plant proteins ---- Zein-alpha Z4 Source.22: DFBPPR6850 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta Source.23: DFBPPR7221 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta Source.24: DFBPPR16005 ---- Animal proteins ---- Myc proto-oncogene protein Source.25: DFBPPR16099 ---- Animal proteins ---- Alpha-ketoglutarate-dependent dioxygenase FTO Source.26: DFBPPR16555 ---- Animal proteins ---- Natural resistance-associated macrophage protein 1 Source.27: DFBPPR17157 ---- Animal proteins ---- AP2-associated protein kinase 1 Source.28: DFBPPR17570 ---- Animal proteins ---- Clathrin light chain B Source.29: DFBPPR18424 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 2 Source.30: DFBPPR18790 ---- Animal proteins ---- Proto-oncogene vav Source.31: DFBPPR19061 ---- Animal proteins ---- RNA-binding protein 5 Source.32: DFBPPR20337 ---- Animal proteins ---- CST complex subunit STN1 Source.33: DFBPPR21596 ---- Animal proteins ---- Origin recognition complex subunit 2 Source.34: DFBPPR21809 ---- Animal proteins ---- Glycosyltransferase 8 domain-containing protein 1 Source.35: DFBPPR8703 ---- Animal proteins ---- AP2-associated protein kinase 1 Source.36: DFBPPR8704 ---- Animal proteins ---- Myc proto-oncogene protein Source.37: DFBPPR9680 ---- Animal proteins ---- Natural resistance-associated macrophage protein 1 Source.38: DFBPPR10021 ---- Animal proteins ---- NT-3 growth factor receptor Source.39: DFBPPR10128 ---- Animal proteins ---- Focal adhesion kinase 1 Source.40: DFBPPR10894 ---- Animal proteins ---- Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 Source.41: DFBPPR11709 ---- Animal proteins ---- Xin actin-binding repeat-containing protein 1 Source.42: DFBPPR11845 ---- Animal proteins ---- Zinc finger and BTB domain-containing protein 17 Source.43: DFBPPR11948 ---- Animal proteins ---- Nucleolar complex protein 4 homolog Source.44: DFBPPR11996 ---- Animal proteins ---- Homeobox protein Hox-A6 Source.45: DFBPPR12174 ---- Animal proteins ---- Protein CNPPD1 Source.46: DFBPPR14991 ---- Microorganism protein ---- Actin cytoskeleton-regulatory complex protein PAN1 Source.47: DFBPPR7820 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta Source.48: DFBPPR8272 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 25 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Urea-denatured α-zein was almost completely hydrolyzed into small peptides by digestion with 1/100 (w/w) of thermolysin at 37 ℃ for 3h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Power, O., Fernández, A., Norris, R., Riera, F.A., FitzGerald, R.J. Selective enrichment of bioactive properties during ultrafiltration of a tryptic digest of β-lactoglobulin. Journal of Functional Foods. 2014, 9, 38-47. DOI: 10.1016/j.jff.2014.04.002
Other literature(s)	N.D
PubDate	2014