Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1598(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1598
Peptide sequence	YSQQQQ
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Ser-Gln-Gln-Gln-Gln

Single-letter amino acid

YSQQQQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	780.80 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

860 μM

pIC₅₀

-2.934

GRAVY

-2.6833^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Maize protein
Precursor protein	α-Zein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR6037 ---- Plant proteins ---- 19 kDa alpha-zein 19C2 Source.2: DFBPPR6057 ---- Plant proteins ---- Zein-alpha PMS1 Source.3: DFBPPR6081 ---- Plant proteins ---- Zein-alpha 19B1 Source.4: DFBPPR6085 ---- Plant proteins ---- Zein-alpha A30 Source.5: DFBPPR6088 ---- Plant proteins ---- Zein-alpha ZG99 Source.6: DFBPPR6093 ---- Plant proteins ---- Zein-alpha 19C1 Source.7: DFBPPR6094 ---- Plant proteins ---- Zein-alpha PZ19.1 Source.8: DFBPPR6095 ---- Plant proteins ---- Zein-alpha A20 Source.9: DFBPPR6096 ---- Plant proteins ---- Zein-alpha GZ19AB11 Source.10: DFBPPR6101 ---- Plant proteins ---- Zein-alpha M6 Source.11: DFBPPR6110 ---- Plant proteins ---- Zein-alpha Z4 Source.12: DFBPPR6887 ---- Plant proteins ---- Glutenin, low molecular weight subunit
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited low Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 860 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Urea-denatured α-zein was almost completely hydrolyzed into small peptides by digestion with 1/100 (w/w) of thermolysin at 37 ℃ for 3h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Power, O., Fernández, A., Norris, R., Riera, F.A., FitzGerald, R.J. Selective enrichment of bioactive properties during ultrafiltration of a tryptic digest of β-lactoglobulin. Journal of Functional Foods. 2014, 9, 38-47. DOI: 10.1016/j.jff.2014.04.002
Other literature(s)	N.D
PubDate	2014