Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1606(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1606
Peptide sequence	YVVFK
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Val-Val-Phe-Lys

Single-letter amino acid

YVVFK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
655.62 Da	654.81 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.70 ^c

IC₅₀

44 μM

pIC₅₀

-1.643

GRAVY

1.2000^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soybean protein
Precursor protein	Glycinin Gy5
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR4966 ---- Plant proteins ---- Glycinin G5 Source.2: DFBPPR6430 ---- Plant proteins ---- Legumin J Source.3: DFBPPR6455 ---- Plant proteins ---- Legumin K Source.4: DFBPPR6459 ---- Plant proteins ---- Legumin B Source.5: DFBPPR9483 ---- Animal proteins ---- Creatine kinase M-type
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 44 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Soybean proteins were hydrolyzed with protease D3 from the germinating cotyledons of soybeans.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANHY0933
[D2]	DFBPMUFU0453

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Kodera, T., Nio, N. Identification of an Angiotensin I-converting Enzyme Inhibitory Peptides from Protein Hydrolysates by a Soybean Protease and the Antihypertensive Effects of Hydrolysates in 4 Spontaneously Hypertensive Model Rats. Journal of Food Science. 2006, 71, C164-C73. DOI: 10.1111/j.1365-2621.2006.tb15612.x
Other literature(s)	N.D
PubDate	2006