Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1672(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1672
Peptide sequence	LLNPT
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Leu-Leu-Asn-Pro-Thr

Single-letter amino acid

LLNPT

Peptide length

Peptide mass

Experimental mass	Theoretical mass
556.3 Da	556.65 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

> 1000 uM

pIC₅₀

> -3.0

GRAVY

0.3600^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(188-192)
Precursor protein(s) search	Source.1: DFBPPR1276 ---- Plant proteins ---- E3 ubiquitin-protein ligase XB3 Source.2: DFBPPR3348 ---- Plant proteins ---- Probable methionine--tRNA ligase Source.3: DFBPPR4096 ---- Plant proteins ---- Cytochrome c biogenesis protein CCS1, chloroplastic Source.4: DFBPPR6471 ---- Plant proteins ---- Beta-amyrin synthase Source.5: DFBPPR7510 ---- Plant proteins ---- Protein EFFECTOR OF TRANSCRIPTION Source.6: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.7: DFBPPR16568 ---- Animal proteins ---- Beta-lactoglobulin-1 Source.8: DFBPPR16569 ---- Animal proteins ---- Beta-lactoglobulin-2 Source.9: DFBPPR18440 ---- Animal proteins ---- Protein 4.2 Source.10: DFBPPR12820 ---- Animal proteins ---- Endothelin receptor type B Source.11: DFBPPR14404 ---- Marine protein ---- Pyruvate dehydrogenase E1 component subunit alpha
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited very low Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of > 1000 uM (> 557.0 μg/mL).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Peptide obtained by hydrolysis of human milk by use of Pepsin and Pancreatin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX0709
[D2]	DFBPMUFU0476

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Hernández-Ledesma, B., Quirós, A., Amigo, L., Recio, I. Identification of bioactive peptides after digestion of human milk and infant formula with pepsin and pancreatin. International Dairy Journal. 2007, 17, 42-9. DOI: 10.1016/j.idairyj.2005.12.012
Other literature(s)	N.D
PubDate	2007