Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1674(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1674
Peptide sequence	PYPQ
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Pro-Tyr-Pro-Gln

Single-letter amino acid

PYPQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
503.2 Da	503.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

> 1000 uM

pIC₅₀

> -3.0

GRAVY

-2.0000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(170-173)
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0382 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0383 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR0384 ---- Plant protein ---- Alpha-gliadin Source.7: DFBPPR0385 ---- Plant protein ---- Alpha-gliadin Source.8: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.9: DFBPPR0390 ---- Plant protein ---- B3144=ALPHA-gliadin derived CELIAC active peptide Source.10: DFBPPR0391 ---- Plant protein ---- B3143=ALPHA-gliadin derived CELIAC active peptide Source.11: DFBPPR1528 ---- Plant proteins ---- Cyclin-dependent kinase C-2 Source.12: DFBPPR1721 ---- Plant proteins ---- Cyclin-dependent kinase C-1 Source.13: DFBPPR1794 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A2 Source.14: DFBPPR3875 ---- Plant proteins ---- Probable glutamyl endopeptidase, chloroplastic Source.15: DFBPPR4966 ---- Plant proteins ---- Glycinin G5 Source.16: DFBPPR5667 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A2 Source.17: DFBPPR6305 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A2 Source.18: DFBPPR6734 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A2 Source.19: DFBPPR6853 ---- Plant proteins ---- Alpha/beta-gliadin MM1 Source.20: DFBPPR6930 ---- Plant proteins ---- Alpha/beta-gliadin Source.21: DFBPPR6934 ---- Plant proteins ---- Alpha/beta-gliadin clone PW1215 Source.22: DFBPPR6936 ---- Plant proteins ---- Alpha/beta-gliadin A-II Source.23: DFBPPR6937 ---- Plant proteins ---- Alpha/beta-gliadin A-IV Source.24: DFBPPR6938 ---- Plant proteins ---- Alpha/beta-gliadin clone PW8142 Source.25: DFBPPR6939 ---- Plant proteins ---- Alpha/beta-gliadin A-V Source.26: DFBPPR6940 ---- Plant proteins ---- Alpha/beta-gliadin A-III Source.27: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I Source.28: DFBPPR6960 ---- Plant proteins ---- Gamma-gliadin Source.29: DFBPPR6965 ---- Plant proteins ---- Gamma-gliadin B Source.30: DFBPPR7108 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A2 Source.31: DFBPPR7265 ---- Plant proteins ---- Gamma-hordein-3 Source.32: DFBPPR7311 ---- Plant proteins ---- B1-hordein Source.33: DFBPPR7318 ---- Plant proteins ---- B3-hordein Source.34: DFBPPR7335 ---- Plant proteins ---- C-hordein Source.35: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.36: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.37: DFBPPR7668 ---- Milk proteins ---- Beta-casein Source.38: DFBPPR7681 ---- Milk proteins ---- Beta-casein Source.39: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.40: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.41: DFBPPR16232 ---- Animal proteins ---- Lysine-specific demethylase 5D Source.42: DFBPPR17459 ---- Animal proteins ---- Beta-1,4-galactosyltransferase 3 Source.43: DFBPPR17995 ---- Animal proteins ---- Ferrochelatase, mitochondrial Source.44: DFBPPR20891 ---- Animal proteins ---- Protein lifeguard 1 Source.45: DFBPPR21228 ---- Animal proteins ---- Inactive hydroxysteroid dehydrogenase-like protein 1 Source.46: DFBPPR21546 ---- Animal proteins ---- Mediator of RNA polymerase II transcription subunit 29 Source.47: DFBPPR8527 ---- Animal proteins ---- Tumor necrosis factor ligand superfamily member 6 Source.48: DFBPPR8923 ---- Animal proteins ---- Cubilin Source.49: DFBPPR9758 ---- Animal proteins ---- Galanin-like peptide Source.50: DFBPPR10693 ---- Animal proteins ---- Ferrochelatase, mitochondrial Source.51: DFBPPR10819 ---- Animal proteins ---- Ribosomal protein S6 kinase alpha-5 Source.52: DFBPPR11325 ---- Animal proteins ---- Peptidase inhibitor 15 Source.53: DFBPPR11494 ---- Animal proteins ---- T-box-containing protein TBX6L Source.54: DFBPPR11605 ---- Animal proteins ---- DNA repair protein complementing XP-A cells homolog Source.55: DFBPPR11791 ---- Animal proteins ---- Protein shisa-5 Source.56: DFBPPR11883 ---- Animal proteins ---- Hypoxia up-regulated protein 1 Source.57: DFBPPR11981 ---- Animal proteins ---- Histone deacetylase complex subunit SAP130 Source.58: DFBPPR13067 ---- Animal proteins ---- Beta-casein Source.59: DFBPPR15069 ---- Microorganism protein ---- Class E vacuolar protein-sorting machinery protein HSE1 Source.60: DFBPPR7779 ---- Plant protein ---- Photosystem I P700 chlorophyll a apoprotein A2 Source.61: DFBPPR7832 ---- Plant protein ---- Extensin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited very low Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of > 1000 uM (> 504.0 μg/mL).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Peptide obtained by hydrolysis of human milk by use of Pepsin and Pancreatin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX0703
[D2]	DFBPMUFU0478

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Hernández-Ledesma, B., Quirós, A., Amigo, L., Recio, I. Identification of bioactive peptides after digestion of human milk and infant formula with pepsin and pancreatin. International Dairy Journal. 2007, 17, 42-9. DOI: 10.1016/j.idairyj.2005.12.012
Other literature(s)	N.D
PubDate	2007