Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1751(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1751
Peptide sequence	VLRP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-Leu-Arg-Pro

Single-letter amino acid

VLRP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
483.7 Da	483.60 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

0.89 uM

pIC₅₀

0.051

GRAVY

0.4750^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Marine shrimp (Acetes chinensis)
Precursor protein	A. chinensis protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0830 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC6 Source.2: DFBPPR0927 ---- Plant proteins ---- Serine/threonine-protein kinase TOR Source.3: DFBPPR0940 ---- Plant proteins ---- Serine/threonine-protein kinase/endoribonuclease IRE1 Source.4: DFBPPR1055 ---- Plant proteins ---- Phospholipase A1 EG1, chloroplastic/mitochondrial Source.5: DFBPPR1057 ---- Plant proteins ---- Abscisic acid receptor PYL10 Source.6: DFBPPR1256 ---- Plant proteins ---- Cytochrome P450 78A11 Source.7: DFBPPR1293 ---- Plant proteins ---- Copper-transporting ATPase HMA5 Source.8: DFBPPR1393 ---- Plant proteins ---- Serine/threonine-protein kinase Nek6 Source.9: DFBPPR1776 ---- Plant proteins ---- Lon protease homolog, mitochondrial Source.10: DFBPPR1814 ---- Plant proteins ---- Histone deacetylase 2 Source.11: DFBPPR1932 ---- Plant proteins ---- Inactive casein kinase II subunit alpha-2 Source.12: DFBPPR2032 ---- Plant proteins ---- Probable protein phosphatase 2C 5 Source.13: DFBPPR2126 ---- Plant proteins ---- Glutelin type-B 2 Source.14: DFBPPR2318 ---- Plant proteins ---- Probable chromatin-remodeling complex ATPase chain Source.15: DFBPPR2409 ---- Plant proteins ---- Histone deacetylase 3 Source.16: DFBPPR2440 ---- Plant proteins ---- Casein kinase II subunit alpha-2 Source.17: DFBPPR2452 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX9 Source.18: DFBPPR2461 ---- Plant proteins ---- Glutelin type-B 1 Source.19: DFBPPR2525 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX10 Source.20: DFBPPR2588 ---- Plant proteins ---- Putative heat stress transcription factor B-4a Source.21: DFBPPR2683 ---- Plant proteins ---- Exonuclease 1 Source.22: DFBPPR2904 ---- Plant proteins ---- Tryptamine hydroxycinnamoyltransferase 2 Source.23: DFBPPR2956 ---- Plant proteins ---- Tryptamine hydroxycinnamoyltransferase 1 Source.24: DFBPPR2993 ---- Plant proteins ---- Beta-glucosidase 5 Source.25: DFBPPR3086 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX33 Source.26: DFBPPR3163 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX32 Source.27: DFBPPR3358 ---- Plant proteins ---- Glutelin type-B 4 Source.28: DFBPPR3666 ---- Plant proteins ---- Glutelin type-B 5 Source.29: DFBPPR3986 ---- Plant proteins ---- CRS2-associated factor 2, chloroplastic Source.30: DFBPPR4020 ---- Plant proteins ---- Probable cation transporter HKT3 Source.31: DFBPPR4042 ---- Plant proteins ---- Probable cation transporter HKT9 Source.32: DFBPPR4187 ---- Plant proteins ---- Barley B recombinant-like protein C Source.33: DFBPPR4193 ---- Plant proteins ---- Peptidyl-tRNA hydrolase, mitochondrial Source.34: DFBPPR4934 ---- Plant proteins ---- U-box domain-containing protein 70 Source.35: DFBPPR5421 ---- Plant proteins ---- Pyruvate, phosphate dikinase regulatory protein, chloroplastic Source.36: DFBPPR5452 ---- Plant proteins ---- Casein kinase II subunit alpha Source.37: DFBPPR5632 ---- Plant proteins ---- CRS2-associated factor 2, chloroplastic Source.38: DFBPPR5788 ---- Plant proteins ---- Globulin-1 S allele Source.39: DFBPPR6015 ---- Plant proteins ---- Casparian strip membrane protein 2 Source.40: DFBPPR6689 ---- Plant proteins ---- Fructan 1-exohydrolase w1 Source.41: DFBPPR6715 ---- Plant proteins ---- Fructan 1-exohydrolase w3 Source.42: DFBPPR6720 ---- Plant proteins ---- Fructan 1-exohydrolase w2 Source.43: DFBPPR6847 ---- Plant proteins ---- Casparian strip membrane protein 1 Source.44: DFBPPR7230 ---- Plant proteins ---- Fructan 1-exohydrolase Source.45: DFBPPR7683 ---- Milk proteins ---- Lactotransferrin Source.46: DFBPPR7713 ---- Milk proteins ---- Lactotransferrin Source.47: DFBPPR8500 ---- Milk proteins ---- Lactotransferrin Source.48: DFBPPR16178 ---- Animal proteins ---- Wee1-like protein kinase 2 Source.49: DFBPPR16274 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 37 Source.50: DFBPPR16490 ---- Animal proteins ---- Translocon-associated protein subunit beta Source.51: DFBPPR16726 ---- Animal proteins ---- Ral guanine nucleotide dissociation stimulator-like 2 Source.52: DFBPPR16889 ---- Animal proteins ---- NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Source.53: DFBPPR17008 ---- Animal proteins ---- Prolyl endopeptidase FAP Source.54: DFBPPR17232 ---- Animal proteins ---- Angiopoietin-1 receptor Source.55: DFBPPR17233 ---- Animal proteins ---- Angiopoietin-1 receptor Source.56: DFBPPR17411 ---- Animal proteins ---- General transcription and DNA repair factor IIH helicase subunit XPB Source.57: DFBPPR17555 ---- Animal proteins ---- ATP synthase subunit delta, mitochondrial Source.58: DFBPPR17779 ---- Animal proteins ---- Integrin alpha-3 Source.59: DFBPPR17818 ---- Animal proteins ---- Endoribonuclease Dicer Source.60: DFBPPR17909 ---- Animal proteins ---- Solute carrier family 2, facilitated glucose transporter member 8 Source.61: DFBPPR18121 ---- Animal proteins ---- Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial Source.62: DFBPPR18161 ---- Animal proteins ---- Disintegrin and metalloproteinase domain-containing protein 2 Source.63: DFBPPR18194 ---- Animal proteins ---- Synapse differentiation-inducing gene protein 1 Source.64: DFBPPR18288 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 37 Source.65: DFBPPR18385 ---- Animal proteins ---- CTD nuclear envelope phosphatase 1 Source.66: DFBPPR18588 ---- Animal proteins ---- CD166 antigen Source.67: DFBPPR19323 ---- Animal proteins ---- WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 Source.68: DFBPPR19449 ---- Animal proteins ---- SCO-spondin Source.69: DFBPPR19518 ---- Animal proteins ---- Rab GTPase-binding effector protein 2 Source.70: DFBPPR19844 ---- Animal proteins ---- Prosalusin Source.71: DFBPPR20153 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform Source.72: DFBPPR20187 ---- Animal proteins ---- Nitric oxide synthase-interacting protein Source.73: DFBPPR20284 ---- Animal proteins ---- Galactose-3-O-sulfotransferase 3 Source.74: DFBPPR20418 ---- Animal proteins ---- Alpha-1B-glycoprotein Source.75: DFBPPR20433 ---- Animal proteins ---- Interleukin-22 receptor subunit alpha-1 Source.76: DFBPPR20476 ---- Animal proteins ---- Translocon-associated protein subunit beta Source.77: DFBPPR20505 ---- Animal proteins ---- T-box transcription factor TBX6 Source.78: DFBPPR20595 ---- Animal proteins ---- LHFPL tetraspan subfamily member 4 protein Source.79: DFBPPR20681 ---- Animal proteins ---- G-protein coupled bile acid receptor 1 Source.80: DFBPPR20706 ---- Animal proteins ---- Chloride channel CLIC-like protein 1 Source.81: DFBPPR20766 ---- Animal proteins ---- Torsin-2A Source.82: DFBPPR20815 ---- Animal proteins ---- Translation initiation factor IF-3, mitochondrial Source.83: DFBPPR20985 ---- Animal proteins ---- Transmembrane protein 131-like Source.84: DFBPPR21314 ---- Animal proteins ---- KIF-binding protein Source.85: DFBPPR21350 ---- Animal proteins ---- Nuclear apoptosis-inducing factor 1 Source.86: DFBPPR21515 ---- Animal proteins ---- P2Y purinoceptor 2 Source.87: DFBPPR21522 ---- Animal proteins ---- ATP-dependent RNA helicase DQX1 Source.88: DFBPPR22445 ---- Animal proteins ---- Tetratricopeptide repeat protein 1 Source.89: DFBPPR22661 ---- Animal proteins ---- Uncharacterized protein C2orf42 homolog Source.90: DFBPPR8808 ---- Animal proteins ---- Cytochrome P450 7A1 Source.91: DFBPPR8887 ---- Animal proteins ---- Transforming growth factor-beta-induced protein ig-h3 Source.92: DFBPPR9187 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 37 Source.93: DFBPPR9436 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform Source.94: DFBPPR9437 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform Source.95: DFBPPR9592 ---- Animal proteins ---- Sodium/nucleoside cotransporter 1 Source.96: DFBPPR9600 ---- Animal proteins ---- P2Y purinoceptor 2 Source.97: DFBPPR9727 ---- Animal proteins ---- FAST kinase domain-containing protein 4 Source.98: DFBPPR10027 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1D Source.99: DFBPPR10612 ---- Animal proteins ---- Carboxypeptidase Z Source.100: DFBPPR10668 ---- Animal proteins ---- E3 UFM1-protein ligase 1 Source.101: DFBPPR10732 ---- Animal proteins ---- General transcription and DNA repair factor IIH helicase subunit XPB Source.102: DFBPPR10953 ---- Animal proteins ---- DNA repair and recombination protein RAD54-like Source.103: DFBPPR11194 ---- Animal proteins ---- Steroidogenic acute regulatory protein, mitochondrial Source.104: DFBPPR11720 ---- Animal proteins ---- Interleukin-17 receptor D Source.105: DFBPPR11915 ---- Animal proteins ---- LysM and putative peptidoglycan-binding domain-containing protein 3 Source.106: DFBPPR11938 ---- Animal proteins ---- Nuclear apoptosis-inducing factor 1 Source.107: DFBPPR11961 ---- Animal proteins ---- KIF-binding protein Source.108: DFBPPR11981 ---- Animal proteins ---- Histone deacetylase complex subunit SAP130 Source.109: DFBPPR12063 ---- Animal proteins ---- Centromere protein M Source.110: DFBPPR12310 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1C Source.111: DFBPPR12321 ---- Animal proteins ---- Voltage-dependent N-type calcium channel subunit alpha-1B Source.112: DFBPPR12325 ---- Animal proteins ---- Glucocorticoid receptor Source.113: DFBPPR12388 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1S Source.114: DFBPPR12396 ---- Animal proteins ---- Voltage-dependent P/Q-type calcium channel subunit alpha-1A Source.115: DFBPPR12422 ---- Animal proteins ---- Voltage-dependent R-type calcium channel subunit alpha-1E Source.116: DFBPPR12434 ---- Animal proteins ---- Aminopeptidase N Source.117: DFBPPR12541 ---- Animal proteins ---- Disintegrin and metalloproteinase domain-containing protein 2 Source.118: DFBPPR12847 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform Source.119: DFBPPR13053 ---- Animal proteins ---- G-protein coupled bile acid receptor 1 Source.120: DFBPPR13822 ---- Animal proteins ---- Steroidogenic acute regulatory protein, mitochondrial Source.121: DFBPPR14003 ---- Animal proteins ---- Dihydropyridine-sensitive L-type skeletal muscle calcium channel subunit alpha-1 Source.122: DFBPPR14448 ---- Marine protein ---- 50S ribosomal protein L2, chloroplastic Source.123: DFBPPR14879 ---- Microorganism protein ---- Pentafunctional AROM polypeptide Source.124: DFBPPR15029 ---- Microorganism protein ---- Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase Source.125: DFBPPR15115 ---- Microorganism protein ---- Helicase SWR1 Source.126: DFBPPR15274 ---- Microorganism protein ---- ATP-dependent RNA helicase DBP7 Source.127: DFBPPR15431 ---- Microorganism protein ---- RNA exonuclease 3 Source.128: DFBPPR7752 ---- Plant protein ---- Trans-cinnamate 4-monooxygenase Source.129: DFBPPR7828 ---- Plant protein ---- Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial Source.130: DFBPPR7893 ---- Plant protein ---- Casparian strip membrane protein 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited very strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 0.89 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Peptides were synthesized using a solid-phase method on a CS536 peptide synthesizer (CS Bio Company, USA) by CL.(XIAN)Bio-Scientfic.Co., LTD.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The novel peptides from hydrolysate of A. chinensis and some of their derived peptides with high ACE inhibitory activity probably have potential in the treatment of hypertension or in clinical nutrition.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Hai-Lun H, Xiu-Lan C, Cai-Yun S, Yu-Zhong Z, Bai-Cheng Z. Analysis of novel angiotensin-I-converting enzyme inhibitory peptides from protease-hydrolyzed marine shrimp Acetes chinensis. J Pept Sci. 2006 Nov;12(11):726-33. PMID: 16981241 DOI: 10.1002/psc.789
Other literature(s)	N.D
PubDate	2006