Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1776(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1776
Peptide sequence	APGAPGPVG
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ala-Pro-Gly-Ala-Pro-Gly-Pro-Val-Gly

Single-letter amino acid

APGAPGPVG

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	721.81 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

16.2 ± 3.8 μM

pIC₅₀

-1.21

GRAVY

0.2000^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Poultry, Chicken
Precursor protein	Collagen alpha-1(I) chain
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR16048 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.2: DFBPPR17360 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.3: DFBPPR9981 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.4: DFBPPR9985 ---- Animal proteins ---- Collagen alpha-1(II) chain
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 16.2 ± 3.8 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	In vitro simulated gastrointestinal digestion (Trypsin + Pepsin + α-Chymotrypsin) was performed to evaluate changes in bioactive properties of Poultry protein hydrolysate HCP Premium P150 (PPH) showing strong antioxidant and moderate ACE-inhibitory activity.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The digested Poultry protein hydrolysate HCP Premium P150 proved to be a rich source of antioxidant and ACE inhibiting molecules and could be a potential new food ingredient used for prevention or treatment of socially significant diseases.

Database cross-references

DFBP

[D1]	DFBPANOX0722
[D2]	DFBPMUFU0521

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Anna, T., Alexey, K., Anna, B., Vyacheslav, K., Mikhail, T., Ulia, M. Effect of in Vitro Gastrointestinal Digestion on Bioactivity of Poultry Protein Hydrolysate. Current Research in Nutrition and Food Science Journal. 2016, 4, 77-86. DOI: 10.12944/CRNFSJ.4.Special-Issue-October.10
Other literature(s)	N.D
PubDate	2016