The fraction F3.10 of water-soluble extracts from Manchego cheeses (containing peptide IPY) showed potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity, so the peptide was a potential ACE-inhibitory peptide, as shown in Table 1.
Table 1. Identification of the peptides contained in the analytical RP-HPLC fractions with ACE-inhibitory activity
Fraction
| Protein fragment
| Sequence
| Protein content (μg/mL) a
| % ACE inhibition a
| F3.10
| αs2-casein f(202-204) | IPY
| 7.7 ± 0.4
| 23.7 ± 5.9
| αs2-casein f(176-179) | AWPQ
| various casein
| FP
| a Average values ± standard deviation (n = 3).
| |