Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1835(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1835
Peptide sequence	ASLLPPY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ala-Ser-Leu-Leu-Pro-Pro-Tyr

Single-letter amino acid

ASLLPPY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
759.42 Da	759.88 Da ^c

Net charge

Isoelectric point (pI)

5.92 ^c

IC₅₀

334 μM

pIC₅₀

-2.524

GRAVY

0.5857^c

Hydrophilic residue ratio

71.43%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Tomato
Precursor protein	Seed protein (by-products)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR6029 ---- Plant proteins ---- Zein-alpha PMS2 Source.2: DFBPPR6037 ---- Plant proteins ---- 19 kDa alpha-zein 19C2 Source.3: DFBPPR6057 ---- Plant proteins ---- Zein-alpha PMS1 Source.4: DFBPPR6081 ---- Plant proteins ---- Zein-alpha 19B1 Source.5: DFBPPR6085 ---- Plant proteins ---- Zein-alpha A30 Source.6: DFBPPR6088 ---- Plant proteins ---- Zein-alpha ZG99 Source.7: DFBPPR6093 ---- Plant proteins ---- Zein-alpha 19C1 Source.8: DFBPPR6094 ---- Plant proteins ---- Zein-alpha PZ19.1 Source.9: DFBPPR6095 ---- Plant proteins ---- Zein-alpha A20 Source.10: DFBPPR6096 ---- Plant proteins ---- Zein-alpha GZ19AB11 Source.11: DFBPPR6101 ---- Plant proteins ---- Zein-alpha M6 Source.12: DFBPPR6110 ---- Plant proteins ---- Zein-alpha Z4
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited moderate Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 334 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Tomato seeds were obtained as by-products and submitted to fermentation with the proteolytic strain Bacillus subtilis A14h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

B. subtilis fermentation of tomato by-products could be a good strategy for obtaining added-value peptides that might be used as an ingredient in functional foods and nutraceuticals.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Moayedi A, Mora L, Aristoy MC, Safari M, Hashemi M, Toldrá F. Peptidomic analysis of antioxidant and ACE-inhibitory peptides obtained from tomato waste proteins fermented using Bacillus subtilis. Food Chem. 2018 Jun 1;250:180-187. PMID: 29412909 DOI: 10.1016/j.foodchem.2018.01.033
Other literature(s)	N.D
PubDate	2018