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List of peptide properties
DFBP ID - DFBPACEI1844(ACE-inhibitory peptide)
DFBP ID DFBPACEI1844
Peptide sequence SQSKVLPVPQ
Type Native peptide
Peptide/Function name ACE-inhibitory peptide
Function-activity relationship
Main bioactivity ACE-inhibitory activity
Otheir bioactivity N.D
Calculated physicochemical properties
Three-letter amino acid Ser-Gln-Ser-Lys-Val-Leu-Pro-Val-Pro-Gln
Single-letter amino acid SQSKVLPVPQ
Peptide length 10
Peptide mass
Experimental mass Theoretical mass
1082.25 Da 1082.26 Da c
Net charge 0.00 c
Isoelectric point (pI) 10.04 c
IC50 92.00 μM
pIC50 -1.964
GRAVY -0.3500 c
Hydrophilic residue ratio 50% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Animal
Organism/Source Bovine sodium caseinate
Precursor protein β-Casein
Residue position

f(166-175)

Precursor protein(s) search
Link-research
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
ACE-inhibitory activity

The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC50 value of 99.00 uM.

Specific target protein(s) Specific Target Protein(s):
Angiotensin-converting enzyme
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Non-bitter taste prediction
SMILES N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O
Preparation method
Mode of preparation

Fermentation

Enzyme(s)/starter culture

The sodium caseinate substrate (2.5%, wt/vol) and glucose (0.5%, wt/vol) were inoculated with Lactobacillus animalis DPC6134 (1%, wt/vol) and incubated at 37°C for 24 h with mixing at 100 rpm and a constant pH of 7 by addition of 0.1 M NaOH.

Stability & Cytotoxicity
Peptide stability
Literature report:

The ACE-inhibitory activity of the L. animalis DPC6134 10-kDa fermentate may be resistant to digestion by the gastrointestinal enzymes pepsin and corolase PP in vitro.

EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
BIOPEP-UWM [D1] -
APD [D2] -
BioPepDB [D3] -
MBPDB [D4] -
Reference(s)
Primary literature Hayes M, Stanton C, Slattery H, O'Sullivan O, Hill C, Fitzgerald GF, Ross RP. Casein fermentate of Lactobacillus animalis DPC6134 contains a range of novel propeptide angiotensin-converting enzyme inhibitors. Appl Environ Microbiol. 2007 Jul;73(14):4658-67.
PMID: 17483275
Other literature(s) N.D
PubDate 2007
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