Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1848(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1848
Peptide sequence	TTMP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Thr-Thr-Met-Pro

Single-letter amino acid

TTMP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	448.53 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.2750^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	α_s1-Casein
Residue position	f(194-197)
Precursor protein(s) search	Source.1: DFBPPR1997 ---- Plant proteins ---- Glucose-6-phosphate isomerase, cytosolic B Source.2: DFBPPR2871 ---- Plant proteins ---- Protein SEMI-ROLLED LEAF 2 Source.3: DFBPPR3237 ---- Plant proteins ---- Arginine decarboxylase 2 Source.4: DFBPPR3550 ---- Plant proteins ---- NRR repressor homolog 2 Source.5: DFBPPR4224 ---- Plant proteins ---- Probable calcium-binding protein CML22 Source.6: DFBPPR7688 ---- Milk proteins ---- Alpha-S1-casein Source.7: DFBPPR7717 ---- Milk proteins ---- Alpha-S1-casein Source.8: DFBPPR8488 ---- Milk proteins ---- Alpha-S1-casein Source.9: DFBPPR16150 ---- Animal proteins ---- Chloride channel protein 1 Source.10: DFBPPR16461 ---- Animal proteins ---- InaD-like protein Source.11: DFBPPR17944 ---- Animal proteins ---- P-selectin Source.12: DFBPPR18029 ---- Animal proteins ---- Collagen alpha-3(IV) chain Source.13: DFBPPR18338 ---- Animal proteins ---- Kappa-type opioid receptor Source.14: DFBPPR19652 ---- Animal proteins ---- Eukaryotic translation initiation factor 3 subunit L Source.15: DFBPPR20788 ---- Animal proteins ---- MICOS complex subunit MIC27 Source.16: DFBPPR20915 ---- Animal proteins ---- Sodium/myo-inositol cotransporter 2 Source.17: DFBPPR20975 ---- Animal proteins ---- 39S ribosomal protein L2, mitochondrial Source.18: DFBPPR21734 ---- Animal proteins ---- TBC1 domain family member 1 Source.19: DFBPPR9399 ---- Animal proteins ---- High affinity copper uptake protein 1 Source.20: DFBPPR9634 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.21: DFBPPR9831 ---- Animal proteins ---- Sodium/myo-inositol cotransporter 2 Source.22: DFBPPR9974 ---- Animal proteins ---- RAF proto-oncogene serine/threonine-protein kinase Source.23: DFBPPR10305 ---- Animal proteins ---- ATP-sensitive inward rectifier potassium channel 12 Source.24: DFBPPR11544 ---- Animal proteins ---- Eukaryotic translation initiation factor 3 subunit L Source.25: DFBPPR12549 ---- Animal proteins ---- Sodium/myo-inositol cotransporter 2 Source.26: DFBPPR13560 ---- Animal proteins ---- P-selectin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity (data not shown).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCSC)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Maruyama, S., Suzuki, H. A Peptide Inhibitor of Angiotensin I Converting Enzyme in the Tryptic Hydrolysate of Casein. Agricultural and Biological Chemistry. 2014, 46, 1393-4. DOI: 10.1080/00021369.1982.10865255
Other literature(s)	[1] Hayes, M., et al., Casein fermentate of Lactobacillus animalis DPC6134 contains a range of novel propeptide angiotensin-converting enzyme inhibitors. Appl Environ Microbiol, 2007. 73(14): p. 4658-67.
PubDate	2014