Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1878(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1878
Peptide sequence	ELIN
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Glu-Leu-Ile-Asn

Single-letter amino acid

ELIN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
487.1 Da	487.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

> 1000 μM

pIC₅₀

> -3.0

GRAVY

0.3250^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Chicken egg white protein
Precursor protein	Ovalbumin
Residue position	f(144-147)
Precursor protein(s) search	Source.1: DFBPPR0857 ---- Plant proteins ---- Mitogen-activated protein kinase 5 Source.2: DFBPPR1271 ---- Plant proteins ---- CBL-interacting protein kinase 23 Source.3: DFBPPR1394 ---- Plant proteins ---- Lipoxygenase 7, chloroplastic Source.4: DFBPPR1413 ---- Plant proteins ---- ATP synthase subunit beta, mitochondrial Source.5: DFBPPR1475 ---- Plant proteins ---- Glutamate receptor 3.1 Source.6: DFBPPR1594 ---- Plant proteins ---- Disease resistance protein Pik-1 Source.7: DFBPPR1894 ---- Plant proteins ---- Clathrin heavy chain 1 Source.8: DFBPPR1898 ---- Plant proteins ---- Clathrin heavy chain 2 Source.9: DFBPPR2441 ---- Plant proteins ---- Disease resistance protein Pikm1-TS Source.10: DFBPPR2473 ---- Plant proteins ---- 2-hydroxyacyl-CoA lyase Source.11: DFBPPR2492 ---- Plant proteins ---- ATP synthase subunit beta, chloroplastic Source.12: DFBPPR2539 ---- Plant proteins ---- Probable aldehyde oxidase 1 Source.13: DFBPPR2834 ---- Plant proteins ---- Sugar transport protein MST3 Source.14: DFBPPR2843 ---- Plant proteins ---- Probable aldehyde oxidase 2 Source.15: DFBPPR2927 ---- Plant proteins ---- Probable aldehyde oxidase 3 Source.16: DFBPPR2958 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX21 Source.17: DFBPPR2977 ---- Plant proteins ---- Nicotinate-nucleotide pyrophosphorylase [carboxylating], chloroplastic Source.18: DFBPPR2983 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX23 Source.19: DFBPPR3327 ---- Plant proteins ---- Putative mannan endo-1,4-beta-mannosidase 9 Source.20: DFBPPR3360 ---- Plant proteins ---- Cycloartenol synthase Source.21: DFBPPR3752 ---- Plant proteins ---- Mannan endo-1,4-beta-mannosidase 7 Source.22: DFBPPR3767 ---- Plant proteins ---- Mannan endo-1,4-beta-mannosidase 8 Source.23: DFBPPR4214 ---- Plant proteins ---- Disease resistance protein Piks-1 Source.24: DFBPPR4826 ---- Plant proteins ---- Probable protein transport Sec1a Source.25: DFBPPR4875 ---- Plant proteins ---- Putative zinc finger CCCH domain-containing protein 64 Source.26: DFBPPR4994 ---- Plant proteins ---- 2-hydroxyisoflavanone synthase Source.27: DFBPPR5002 ---- Plant proteins ---- 2S albumin Source.28: DFBPPR5050 ---- Plant proteins ---- 3,9-dihydroxypterocarpan 6A-monooxygenase Source.29: DFBPPR5059 ---- Plant proteins ---- Phosphoenolpyruvate carboxylase Source.30: DFBPPR5190 ---- Plant proteins ---- ATP synthase subunit beta, chloroplastic Source.31: DFBPPR5224 ---- Plant proteins ---- Cytochrome P450 93A3 Source.32: DFBPPR5282 ---- Plant proteins ---- Cytochrome P450 93A2 Source.33: DFBPPR5570 ---- Plant proteins ---- ATP synthase subunit beta, mitochondrial Source.34: DFBPPR5579 ---- Plant proteins ---- Indole-3-acetaldehyde oxidase Source.35: DFBPPR5709 ---- Plant proteins ---- indolin-2-one monooxygenase Source.36: DFBPPR5873 ---- Plant proteins ---- ATP synthase subunit beta, chloroplastic Source.37: DFBPPR6165 ---- Plant proteins ---- Uncharacterized 33.9 kDa protein in mitochondrial linear 2.3 KB plasmid Source.38: DFBPPR6385 ---- Plant proteins ---- Phosphoenolpyruvate carboxylase Source.39: DFBPPR6423 ---- Plant proteins ---- ATP synthase subunit beta, chloroplastic Source.40: DFBPPR6844 ---- Plant proteins ---- ATP synthase subunit beta, chloroplastic Source.41: DFBPPR7185 ---- Plant proteins ---- ATP synthase subunit beta, chloroplastic Source.42: DFBPPR7186 ---- Plant proteins ---- Photosystem I reaction center subunit III, chloroplastic Source.43: DFBPPR7475 ---- Plant proteins ---- ATP synthase subunit beta, chloroplastic Source.44: DFBPPR7644 ---- Milk proteins ---- Plasma protease C1 inhibitor Source.45: DFBPPR8191 ---- Plant proteins ---- L-ascorbate oxidase Source.46: DFBPPR16123 ---- Animal proteins ---- Coiled-coil domain-containing protein 66 Source.47: DFBPPR16292 ---- Animal proteins ---- Protein unc-119 homolog A Source.48: DFBPPR16461 ---- Animal proteins ---- InaD-like protein Source.49: DFBPPR16495 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 52 homolog Source.50: DFBPPR16575 ---- Animal proteins ---- Dystrophin Source.51: DFBPPR16798 ---- Animal proteins ---- ATP synthase subunit beta, mitochondrial Source.52: DFBPPR16853 ---- Animal proteins ---- Protein S100-B Source.53: DFBPPR17035 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Source.54: DFBPPR17061 ---- Animal proteins ---- Interferon-induced GTP-binding protein Mx2 Source.55: DFBPPR17296 ---- Animal proteins ---- Dynamin-2 Source.56: DFBPPR17303 ---- Animal proteins ---- Phosphatidylinositol 3-kinase regulatory subunit alpha Source.57: DFBPPR17429 ---- Animal proteins ---- EEF1AKMT4-ECE2 readthrough transcript protein Source.58: DFBPPR17440 ---- Animal proteins ---- Clathrin heavy chain 1 Source.59: DFBPPR17645 ---- Animal proteins ---- Endothelin-converting enzyme 2 Source.60: DFBPPR17717 ---- Animal proteins ---- Unconventional myosin-Ia Source.61: DFBPPR17872 ---- Animal proteins ---- Probable ATP-dependent RNA helicase DDX4 Source.62: DFBPPR17969 ---- Animal proteins ---- Triosephosphate isomerase Source.63: DFBPPR18044 ---- Animal proteins ---- Sorting nexin-5 Source.64: DFBPPR18424 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 2 Source.65: DFBPPR18553 ---- Animal proteins ---- Factor XIIa inhibitor Source.66: DFBPPR18605 ---- Animal proteins ---- Mitochondrial proton/calcium exchanger protein Source.67: DFBPPR18632 ---- Animal proteins ---- Syntaxin-4 Source.68: DFBPPR18739 ---- Animal proteins ---- Bone morphogenetic protein 3 Source.69: DFBPPR18757 ---- Animal proteins ---- Mevalonate kinase Source.70: DFBPPR19191 ---- Animal proteins ---- Protein unc-119 homolog A Source.71: DFBPPR19199 ---- Animal proteins ---- Integrin alpha-5 Source.72: DFBPPR19253 ---- Animal proteins ---- Limbin Source.73: DFBPPR19295 ---- Animal proteins ---- 26S proteasome regulatory subunit 7 Source.74: DFBPPR19346 ---- Animal proteins ---- Cylicin-1 Source.75: DFBPPR19482 ---- Animal proteins ---- Ubiquitin-conjugating enzyme E2 Q2 Source.76: DFBPPR19908 ---- Animal proteins ---- DNA replication licensing factor MCM6 Source.77: DFBPPR20025 ---- Animal proteins ---- Importin subunit alpha-7 Source.78: DFBPPR20265 ---- Animal proteins ---- Histone-lysine N-methyltransferase EZH1 Source.79: DFBPPR20482 ---- Animal proteins ---- Tripartite motif-containing protein 54 Source.80: DFBPPR20827 ---- Animal proteins ---- Kelch-like protein 13 Source.81: DFBPPR20947 ---- Animal proteins ---- COP9 signalosome complex subunit 3 Source.82: DFBPPR21068 ---- Animal proteins ---- 40S ribosomal protein S5 Source.83: DFBPPR21282 ---- Animal proteins ---- Spindle and centriole-associated protein 1 Source.84: DFBPPR21376 ---- Animal proteins ---- Calponin-3 Source.85: DFBPPR21397 ---- Animal proteins ---- Leucine zipper transcription factor-like protein 1 Source.86: DFBPPR21473 ---- Animal proteins ---- TRPM8 channel-associated factor 1 Source.87: DFBPPR21577 ---- Animal proteins ---- Actin-like protein 7A Source.88: DFBPPR22044 ---- Animal proteins ---- Transgelin-2 Source.89: DFBPPR22269 ---- Animal proteins ---- Protein preY, mitochondrial Source.90: DFBPPR22621 ---- Animal proteins ---- Leucine-rich repeat-containing protein 72 Source.91: DFBPPR9132 ---- Animal proteins ---- Dystrophin Source.92: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Source.93: DFBPPR10034 ---- Animal proteins ---- Tyrosine-protein kinase SYK Source.94: DFBPPR10036 ---- Animal proteins ---- Acetyl-CoA carboxylase Source.95: DFBPPR10128 ---- Animal proteins ---- Focal adhesion kinase 1 Source.96: DFBPPR10509 ---- Animal proteins ---- Calponin-1 Source.97: DFBPPR10601 ---- Animal proteins ---- DNA mismatch repair protein Msh6 Source.98: DFBPPR10679 ---- Animal proteins ---- ATP synthase subunit beta, mitochondrial Source.99: DFBPPR10807 ---- Animal proteins ---- Dystrophin Source.100: DFBPPR10997 ---- Animal proteins ---- Tubulin-specific chaperone D Source.101: DFBPPR11002 ---- Animal proteins ---- Cartilage matrix protein Source.102: DFBPPR11044 ---- Animal proteins ---- Mitochondrial proton/calcium exchanger protein Source.103: DFBPPR11073 ---- Animal proteins ---- Axin-1 Source.104: DFBPPR11136 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase gamma Source.105: DFBPPR11298 ---- Animal proteins ---- Transcription elongation factor SPT5 Source.106: DFBPPR11604 ---- Animal proteins ---- Centromere protein I Source.107: DFBPPR11850 ---- Animal proteins ---- COP9 signalosome complex subunit 3 Source.108: DFBPPR12362 ---- Animal proteins ---- Transient receptor potential cation channel subfamily V member 1 Source.109: DFBPPR12443 ---- Animal proteins ---- DNA repair protein RAD51 homolog 1 Source.110: DFBPPR12742 ---- Animal proteins ---- Ubiquitin-conjugating enzyme E2 Q2 Source.111: DFBPPR12970 ---- Animal proteins ---- Protein S100-B Source.112: DFBPPR13882 ---- Animal proteins ---- Interferon-induced GTP-binding protein Mx2 Source.113: DFBPPR14000 ---- Animal proteins ---- ATP synthase subunit beta, mitochondrial Source.114: DFBPPR14300 ---- Marine protein ---- ATP synthase subunit beta, chloroplastic Source.115: DFBPPR14370 ---- Marine protein ---- DNA-directed RNA polymerase subunit beta'' Source.116: DFBPPR14372 ---- Marine protein ---- ATP synthase subunit beta, chloroplastic Source.117: DFBPPR14412 ---- Marine protein ---- Elongation factor Tu, chloroplastic Source.118: DFBPPR14506 ---- Marine protein ---- Photosystem I reaction center subunit IV Source.119: DFBPPR14895 ---- Microorganism protein ---- Chromatin-remodeling ATPase INO80 Source.120: DFBPPR14934 ---- Microorganism protein ---- ATP synthase subunit beta, mitochondrial Source.121: DFBPPR14943 ---- Microorganism protein ---- Nuclear protein localization protein 4 Source.122: DFBPPR15088 ---- Microorganism protein ---- Autophagy-related protein 13 Source.123: DFBPPR15188 ---- Microorganism protein ---- DNA mismatch repair protein MSH3 Source.124: DFBPPR15255 ---- Microorganism protein ---- Ribosome biogenesis protein ERB1 Source.125: DFBPPR15383 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 17 Source.126: DFBPPR15494 ---- Microorganism protein ---- Protein STU1 Source.127: DFBPPR15499 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 12 Source.128: DFBPPR15500 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 5 Source.129: DFBPPR15560 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 13 Source.130: DFBPPR15742 ---- Microorganism protein ---- High-osmolarity-induced transcription protein 1 Source.131: DFBPPR15788 ---- Microorganism protein ---- Maintenance of telomere capping protein 2 Source.132: DFBPPR15826 ---- Microorganism protein ---- Galactose-1-phosphate uridylyltransferase Source.133: DFBPPR15869 ---- Microorganism protein ---- Phenylalanine ammonia-lyase Source.134: DFBPPR7808 ---- Plant protein ---- ATP synthase subunit beta, chloroplastic Source.135: DFBPPR8100 ---- Plant protein ---- ATP synthase subunit beta, chloroplastic Source.136: DFBPPR8119 ---- Plant protein ---- Chalcone--flavonone isomerase Source.137: DFBPPR8268 ---- Plant protein ---- ATP synthase subunit beta, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited very low Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of > 1000 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Crude egg white proteins were hydrolyzed with Pepsin (EC 3.4.23.1) for 3 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Results from the present study suggest that the peptic hydrolysate of egg white, its fractions, or isolated peptides can be used in the prophylaxis and prevention of hypertension.

Database cross-references

DFBP

[D1]	DFBPANOX0983
[D2]	DFBPMUFU0582

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Miguel M, Recio I, Gómez-Ruiz JA, Ramos M, López-Fandiño R. Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot. 2004 Sep;67(9):1914-20. PMID: 15453581 DOI: 10.4315/0362-028X-67.9.1914
Other literature(s)	N.D
PubDate	2004