Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2006(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2006
Peptide sequence	VDHV
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-Asp-His-Val

Single-letter amino acid

VDHV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
468.5 Da	468.51 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

4.96 ^c

IC₅₀

461.90 ± 14.29 μM

pIC₅₀

-2.665

GRAVY

0.4250^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Korat crossbred (KC) chicken
Precursor protein	Chicken breast meat
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1222 ---- Plant proteins ---- Protein CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR 1 Source.2: DFBPPR1349 ---- Plant proteins ---- 15-cis-phytoene desaturase, chloroplastic/chromoplastic Source.3: DFBPPR1421 ---- Plant proteins ---- GDP-mannose 3,5-epimerase 1 Source.4: DFBPPR1906 ---- Plant proteins ---- Ribose-phosphate pyrophosphokinase 1, chloroplastic Source.5: DFBPPR2415 ---- Plant proteins ---- Putative aconitate hydratase, cytoplasmic Source.6: DFBPPR2891 ---- Plant proteins ---- GDP-mannose 3,5-epimerase 2 Source.7: DFBPPR3018 ---- Plant proteins ---- Molybdopterin synthase catalytic subunit Source.8: DFBPPR3086 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX33 Source.9: DFBPPR3130 ---- Plant proteins ---- COP9 signalosome complex subunit 6 Source.10: DFBPPR3163 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX32 Source.11: DFBPPR3697 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 39 Source.12: DFBPPR3946 ---- Plant proteins ---- Transcription factor TGAL10 Source.13: DFBPPR3980 ---- Plant proteins ---- 4-coumarate--CoA ligase-like 7 Source.14: DFBPPR4144 ---- Plant proteins ---- Origin of replication complex subunit 2 Source.15: DFBPPR4164 ---- Plant proteins ---- Probable NADPH:quinone oxidoreductase 1 Source.16: DFBPPR4286 ---- Plant proteins ---- Cyclin-T1-2 Source.17: DFBPPR4402 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 25 Source.18: DFBPPR4885 ---- Plant proteins ---- REF/SRPP-like protein Os05g0151300/LOC_Os05g05940 Source.19: DFBPPR8197 ---- Plant proteins ---- Aconitate hydratase, cytoplasmic Source.20: DFBPPR16108 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Source.21: DFBPPR16693 ---- Animal proteins ---- Cingulin Source.22: DFBPPR17064 ---- Animal proteins ---- Unconventional myosin-Ic Source.23: DFBPPR17365 ---- Animal proteins ---- Phospholipase ABHD3 Source.24: DFBPPR17773 ---- Animal proteins ---- Adenosylhomocysteinase 3 Source.25: DFBPPR17874 ---- Animal proteins ---- Endonuclease 8-like 2 Source.26: DFBPPR18596 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Source.27: DFBPPR18776 ---- Animal proteins ---- Nucleoporin GLE1 Source.28: DFBPPR19382 ---- Animal proteins ---- Arrestin-C Source.29: DFBPPR21142 ---- Animal proteins ---- Mannose-1-phosphate guanyltransferase beta Source.30: DFBPPR21407 ---- Animal proteins ---- Ribosome biogenesis protein BRX1 homolog Source.31: DFBPPR21562 ---- Animal proteins ---- COP9 signalosome complex subunit 6 Source.32: DFBPPR21662 ---- Animal proteins ---- Ornithine decarboxylase antizyme 1 Source.33: DFBPPR22314 ---- Animal proteins ---- TM2 domain-containing protein 2 Source.34: DFBPPR22397 ---- Animal proteins ---- NHL repeat-containing protein 2 Source.35: DFBPPR22696 ---- Animal proteins ---- Protein FAM228B Source.36: DFBPPR8982 ---- Animal proteins ---- Mannose-1-phosphate guanyltransferase beta Source.37: DFBPPR9140 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Source.38: DFBPPR9351 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Source.39: DFBPPR9763 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform Source.40: DFBPPR9796 ---- Animal proteins ---- Arrestin-C Source.41: DFBPPR9821 ---- Animal proteins ---- COP9 signalosome complex subunit 6 Source.42: DFBPPR10300 ---- Animal proteins ---- Unconventional myosin-Va Source.43: DFBPPR10471 ---- Animal proteins ---- Transcription factor SOX-21 Source.44: DFBPPR10608 ---- Animal proteins ---- Semaphorin-3D Source.45: DFBPPR10811 ---- Animal proteins ---- Chromatin assembly factor 1 subunit A Source.46: DFBPPR11446 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 48 Source.47: DFBPPR11681 ---- Animal proteins ---- Ornithine decarboxylase antizyme 1 Source.48: DFBPPR13363 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 4L Source.49: DFBPPR14081 ---- Marine protein ---- Beta-enolase Source.50: DFBPPR14943 ---- Microorganism protein ---- Nuclear protein localization protein 4 Source.51: DFBPPR15111 ---- Microorganism protein ---- mRNA cap guanine-N7 methyltransferase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide showed low Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 461.90 ± 14.29 μM (Average and standard deviation of 3 triplicate were presented.).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	A simulated in vitro gastrointestinal (GI) digestion (Pepsin + Pancreatin) of Korat crossbred breast meat heated at 70 ℃ for 0.5 h (H-0.5) showed the highest ACE inhibitory activity (91.64 ± 1.89%).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The Korat crossbred (KC) chicken is a new breed that is a cross between a male Thai indigenous chicken and a female broiler.
This study confirmed that chicken breast heated at KC/H-0.5 is a source of health-promoting peptides.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Sangsawad, P., Roytrakul, S., Yongsawatdigul, J. Angiotensin converting enzyme (ACE) inhibitory peptides derived from the simulated in vitro gastrointestinal digestion of cooked chicken breast. Journal of Functional Foods. 2017, 29, 77-83. DOI: 10.1016/j.jff.2016.12.005
Other literature(s)	N.D
PubDate	2017