Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2047(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2047
Peptide sequence	NCW
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Asn-Cys-Trp

Single-letter amino acid

NCW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	421.48 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.85

IC₅₀

35.5 μM

pIC₅₀

-1.55

GRAVY

-0.6333^c

Hydrophilic residue ratio

33.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Mizuhopecten yessoensis
Precursor protein	Myosin
Residue position	f(33–35)
Precursor protein(s) search	Source.1: DFBPPR0917 ---- Plant proteins ---- Ethylene receptor 2 Source.2: DFBPPR1007 ---- Plant proteins ---- Histone-lysine N-methyltransferase EZ1 Source.3: DFBPPR1267 ---- Plant proteins ---- Regulator of telomere elongation helicase 1 homolog Source.4: DFBPPR1333 ---- Plant proteins ---- Endoglucanase 2 Source.5: DFBPPR1455 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 5 [UDP-forming] Source.6: DFBPPR1461 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 3 [UDP-forming] Source.7: DFBPPR1462 ---- Plant proteins ---- Probable cellulose synthase A catalytic subunit 6 [UDP-forming] Source.8: DFBPPR1529 ---- Plant proteins ---- Nitrate reductase [NADH] 1 Source.9: DFBPPR1835 ---- Plant proteins ---- Laccase-15 Source.10: DFBPPR1855 ---- Plant proteins ---- Aminopeptidase M1-B Source.11: DFBPPR1886 ---- Plant proteins ---- Triosephosphate isomerase, cytosolic Source.12: DFBPPR2056 ---- Plant proteins ---- Receptor-like cytoplasmic kinase 176 Source.13: DFBPPR2558 ---- Plant proteins ---- Pyruvate decarboxylase 1 Source.14: DFBPPR2985 ---- Plant proteins ---- Coatomer subunit delta-3 Source.15: DFBPPR3407 ---- Plant proteins ---- Coatomer subunit delta-2 Source.16: DFBPPR3408 ---- Plant proteins ---- Coatomer subunit delta-1 Source.17: DFBPPR3445 ---- Plant proteins ---- Serine/threonine-protein kinase ATR Source.18: DFBPPR3515 ---- Plant proteins ---- Coatomer subunit delta-4 Source.19: DFBPPR3888 ---- Plant proteins ---- Endoglucanase 24 Source.20: DFBPPR4164 ---- Plant proteins ---- Probable NADPH:quinone oxidoreductase 1 Source.21: DFBPPR4228 ---- Plant proteins ---- Probable NADPH:quinone oxidoreductase 2 Source.22: DFBPPR4460 ---- Plant proteins ---- Protein argonaute 3 Source.23: DFBPPR4802 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 34 Source.24: DFBPPR4900 ---- Plant proteins ---- Histone-lysine N-methyltransferase CLF Source.25: DFBPPR5046 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 2 Source.26: DFBPPR5049 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 1 Source.27: DFBPPR5115 ---- Plant proteins ---- Digalactosyldiacylglycerol synthase 2, chloroplastic Source.28: DFBPPR5467 ---- Plant proteins ---- Nitrate reductase [NADH] Source.29: DFBPPR5595 ---- Plant proteins ---- Calcium sensing receptor, chloroplastic Source.30: DFBPPR5616 ---- Plant proteins ---- Triosephosphate isomerase, cytosolic Source.31: DFBPPR5695 ---- Plant proteins ---- Nitrate reductase [NADH] 3 Source.32: DFBPPR5733 ---- Plant proteins ---- Pyruvate decarboxylase 1 Source.33: DFBPPR5818 ---- Plant proteins ---- Histone-lysine N-methyltransferase EZ2 Source.34: DFBPPR5897 ---- Plant proteins ---- Histone-lysine N-methyltransferase EZ1 Source.35: DFBPPR5904 ---- Plant proteins ---- Histone-lysine N-methyltransferase EZ3 Source.36: DFBPPR7039 ---- Plant proteins ---- Nitrate reductase [NAD(P)H] Source.37: DFBPPR7051 ---- Plant proteins ---- Triosephosphate isomerase, cytosolic Source.38: DFBPPR7058 ---- Plant proteins ---- Nitrate reductase [NADH] Source.39: DFBPPR7062 ---- Plant proteins ---- Nitrate reductase [NADH] Source.40: DFBPPR7430 ---- Plant proteins ---- Nitrate reductase [NADH], clone PBNBR1412 Source.41: DFBPPR7432 ---- Plant proteins ---- Nitrate reductase [NADH], clone PBNBR1405 Source.42: DFBPPR7500 ---- Plant proteins ---- L-ascorbate oxidase homolog Source.43: DFBPPR7603 ---- Milk proteins ---- Xanthine dehydrogenase/oxidase Source.44: DFBPPR8188 ---- Plant proteins ---- Nitrate reductase [NADH] Source.45: DFBPPR8457 ---- Plant proteins ---- Triosephosphate isomerase, cytosolic Source.46: DFBPPR8498 ---- Milk proteins ---- Xanthine dehydrogenase/oxidase Source.47: DFBPPR16943 ---- Animal proteins ---- Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Source.48: DFBPPR17166 ---- Animal proteins ---- Cyclic nucleotide-gated cation channel beta-1 Source.49: DFBPPR17329 ---- Animal proteins ---- Steroidogenic factor 1 Source.50: DFBPPR17538 ---- Animal proteins ---- Septin-7 Source.51: DFBPPR17752 ---- Animal proteins ---- Follistatin Source.52: DFBPPR17826 ---- Animal proteins ---- RNA-splicing ligase RtcB homolog Source.53: DFBPPR17887 ---- Animal proteins ---- Bifunctional purine biosynthesis protein ATIC Source.54: DFBPPR17980 ---- Animal proteins ---- Serine/threonine-protein kinase haspin Source.55: DFBPPR17997 ---- Animal proteins ---- Tyrosine-protein kinase CSK Source.56: DFBPPR18089 ---- Animal proteins ---- Coatomer subunit delta Source.57: DFBPPR18759 ---- Animal proteins ---- Neuronal-specific septin-3 Source.58: DFBPPR18823 ---- Animal proteins ---- T-cell-specific surface glycoprotein CD28 Source.59: DFBPPR18836 ---- Animal proteins ---- Calcitonin gene-related peptide type 1 receptor Source.60: DFBPPR18837 ---- Animal proteins ---- Coatomer subunit alpha Source.61: DFBPPR18967 ---- Animal proteins ---- Krev interaction trapped protein 1 Source.62: DFBPPR19121 ---- Animal proteins ---- Adhesion G-protein coupled receptor D1 Source.63: DFBPPR19151 ---- Animal proteins ---- 28S ribosomal protein S27, mitochondrial Source.64: DFBPPR19204 ---- Animal proteins ---- Regulator of G-protein signaling 7 Source.65: DFBPPR19497 ---- Animal proteins ---- G1/S-specific cyclin-E2 Source.66: DFBPPR19735 ---- Animal proteins ---- Complement component C7 Source.67: DFBPPR19978 ---- Animal proteins ---- 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase Source.68: DFBPPR20027 ---- Animal proteins ---- DnaJ homolog subfamily B member 12 Source.69: DFBPPR20129 ---- Animal proteins ---- 2-aminomuconic semialdehyde dehydrogenase Source.70: DFBPPR20247 ---- Animal proteins ---- Claudin-15 Source.71: DFBPPR20345 ---- Animal proteins ---- DnaJ homolog subfamily B member 14 Source.72: DFBPPR20574 ---- Animal proteins ---- Transmembrane protein 201 Source.73: DFBPPR20926 ---- Animal proteins ---- 60S ribosomal protein L8 Source.74: DFBPPR21416 ---- Animal proteins ---- 39S ribosomal protein L32, mitochondrial Source.75: DFBPPR21446 ---- Animal proteins ---- Spermatid-specific manchette-related protein 1 Source.76: DFBPPR21571 ---- Animal proteins ---- Mitochondrial fission regulator 2 Source.77: DFBPPR21656 ---- Animal proteins ---- Thioredoxin domain-containing protein 11 Source.78: DFBPPR22231 ---- Animal proteins ---- DnaJ homolog subfamily C member 12 Source.79: DFBPPR22396 ---- Animal proteins ---- Actin-related protein 10 Source.80: DFBPPR22630 ---- Animal proteins ---- Posterior pituitary peptide Source.81: DFBPPR8600 ---- Animal proteins ---- Steroidogenic factor 1 Source.82: DFBPPR8637 ---- Animal proteins ---- Phosphoinositide 3-kinase regulatory subunit 5 Source.83: DFBPPR8690 ---- Animal proteins ---- Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Source.84: DFBPPR8810 ---- Animal proteins ---- Follistatin Source.85: DFBPPR8896 ---- Animal proteins ---- Heat-stable enterotoxin receptor Source.86: DFBPPR8984 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.87: DFBPPR9238 ---- Animal proteins ---- RNA-splicing ligase RtcB homolog Source.88: DFBPPR10063 ---- Animal proteins ---- Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Source.89: DFBPPR10086 ---- Animal proteins ---- Phosphoenolpyruvate carboxykinase [GTP], mitochondrial Source.90: DFBPPR10217 ---- Animal proteins ---- Follistatin Source.91: DFBPPR10225 ---- Animal proteins ---- Neuropilin-1 Source.92: DFBPPR10587 ---- Animal proteins ---- Beta-tectorin Source.93: DFBPPR10690 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.94: DFBPPR11171 ---- Animal proteins ---- Coatomer subunit delta Source.95: DFBPPR11224 ---- Animal proteins ---- Nuclear receptor subfamily 5 group A member 2 Source.96: DFBPPR11611 ---- Animal proteins ---- Potassium channel subfamily T member 1 Source.97: DFBPPR11708 ---- Animal proteins ---- Phosphoinositide 3-kinase regulatory subunit 5 Source.98: DFBPPR11817 ---- Animal proteins ---- Meiosis regulator and mRNA stability factor 1 Source.99: DFBPPR11998 ---- Animal proteins ---- Kelch-like protein 15 Source.100: DFBPPR12039 ---- Animal proteins ---- Protein GOLM2 Source.101: DFBPPR12486 ---- Animal proteins ---- Adenylate cyclase type 10 Source.102: DFBPPR12494 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.103: DFBPPR12540 ---- Animal proteins ---- Calcitonin receptor Source.104: DFBPPR12960 ---- Animal proteins ---- Synaptophysin-like protein 2 Source.105: DFBPPR13203 ---- Animal proteins ---- Follistatin Source.106: DFBPPR13285 ---- Animal proteins ---- Steroidogenic factor 1 Source.107: DFBPPR13876 ---- Animal proteins ---- Follistatin Source.108: DFBPPR14003 ---- Animal proteins ---- Dihydropyridine-sensitive L-type skeletal muscle calcium channel subunit alpha-1 Source.109: DFBPPR14183 ---- Marine protein ---- Pleckstrin homology-like domain family A member 2 Source.110: DFBPPR14564 ---- Marine protein ---- Piwi-like protein 2 Source.111: DFBPPR15119 ---- Microorganism protein ---- Protein SEY1 Source.112: DFBPPR15580 ---- Microorganism protein ---- Oligosaccharide translocation protein RFT1 Source.113: DFBPPR15581 ---- Microorganism protein ---- Maintenance of telomere capping protein 6 Source.114: DFBPPR15631 ---- Microorganism protein ---- Pre-mRNA-splicing factor RSE1 Source.115: DFBPPR15763 ---- Microorganism protein ---- ATPase expression protein 3 Source.116: DFBPPR8040 ---- Plant protein ---- Nitrate reductase [NADH] 1 Source.117: DFBPPR8041 ---- Plant protein ---- Nitrate reductase [NADH] 2 Source.118: DFBPPR8069 ---- Plant protein ---- Endoglucanase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity

The peptide NCW showed potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 35.5 μM.
The results suggested that the peptide NCW comes into contact with Zn 701, Tyr 523, His 383, Glu 384, Glu 411, and His 387. The higher inhibitory potency of NCW might be attributed to the formation of more hydrogen bonds with the ACE’s active site.
The 3-D structure of the human ACE was imported from the Protein Data Bank (1O86.pdb, a crystal structure of the human ACE).

Specific target protein(s)

Specific Target Protein(s):
Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Myosin was hydrolysed with pepsin and trypsin in silico.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	nontoxic
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yu Z , Wu S , Zhao W , Ding L , Shiuan D , Chen F , Li J , Liu J . Identification and the molecular mechanism of a novel myosin-derived ACE inhibitory peptide. Food Funct. 2018 Jan 24;9(1):364-370. PMID: 29210412 DOI: 10.1039/c7fo01558e
Other literature(s)	N.D
PubDate	2018