Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2065(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2065
Peptide sequence	DELQDKIHPF
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide, PIN-94

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Asp-Glu-Leu-Gln-Asp-Lys-Ile-His-Pro-Phe

Single-letter amino acid

DELQDKIHPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
1240.6088 Da	1241.35 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

4.33 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.1600^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Milk protein
Organism/Source	Buffalo milk
Precursor protein	Buffalo milk protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.3: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide was purified from buffalo milk protein, fermented with LC (Lactobacillus casei). It's a potential ACE-inhibitory peptide (IC₅₀ value of synthetic peptide was not determined).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Bovine milk protein, fermented with LC (Lactobacillus casei) was used to evaluate its ACE inhibitory activity in-vitro.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

In this way, middle-aged healthy buffalo can produce higher levels of milk protein and proved to be a better selection for the production of functional food to reduce hypertension.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Aslam, M.Z., Aslam, M.S., Firdos, S., Ghous, G., Firdos, G., Hongfei, Z., et al. Role of Bioactive Peptides in Reducing the Severity of Hypertension with the Inhibition of ACE. International Journal of Peptide Research and Therapeutics. 2019, 25, 1639-49. DOI: 10.1007/s10989-018-09806-y
Other literature(s)	N.D
PubDate	2019