Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2163(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2163
Peptide sequence	PNYHPSPR
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Pro-Asn-Tyr-His-Pro-Ser-Pro-Arg

Single-letter amino acid

PNYHPSPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
967.0 Da	967.04 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.82 ^c

IC₅₀

18.98 ± 0.26 μM

pIC₅₀

-1.278

GRAVY

-2.2625^c

Hydrophilic residue ratio

37.5%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Sesame (Sesamum indicum L.)
Precursor protein	11S globulin seed storage protein 2 precursor
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR8428 ---- Plant proteins ---- 11S globulin seed storage protein 2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide PNYHPSPR exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 18.98 ± 0.26 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The sesame protein was enzymatically hydrolyzed by pepsin, trypsin, and α-chymotrypsin through simulated gastrointestinal digestion in vitro.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Sesame protein can be a promising ACE inhibitor in the prevention and treatment of hypertension. In addition, PNYHPSPR can be used as a potential nutraceutical for development of functional foods.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wang R, Lu X, Sun Q, Gao J, Ma L, Huang J. Novel ACE Inhibitory Peptides Derived from Simulated Gastrointestinal Digestion in Vitro of Sesame (Sesamum indicum L.) Protein and Molecular Docking Study. Int J Mol Sci. 2020 Feb 5;21(3):1059. PMID: 32033479 DOI: 10.3390/ijms21031059
Other literature(s)	N.D
PubDate	2020