Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2217(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2217
Peptide sequence	KFKY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Phe-Lys-Tyr

Single-letter amino acid

KFKY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	584.71 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.18 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.5750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant, Marine
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica proteins (LJPs)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1775 ---- Plant proteins ---- B3 domain-containing protein IDEF1 Source.2: DFBPPR3540 ---- Plant proteins ---- Auxin transporter-like protein 2 Source.3: DFBPPR3610 ---- Plant proteins ---- Auxin transporter-like protein 1 Source.4: DFBPPR8370 ---- Plant proteins ---- Maturase K Source.5: DFBPPR16068 ---- Animal proteins ---- Cytochrome P450 2E1 Source.6: DFBPPR16132 ---- Animal proteins ---- Signal peptidase complex catalytic subunit SEC11C Source.7: DFBPPR16135 ---- Animal proteins ---- Gap junction alpha-1 protein Source.8: DFBPPR16175 ---- Animal proteins ---- Signal peptidase complex catalytic subunit SEC11A Source.9: DFBPPR16559 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.10: DFBPPR16732 ---- Animal proteins ---- HORMA domain-containing protein 2 Source.11: DFBPPR17098 ---- Animal proteins ---- Cytochrome P450 2E1 Source.12: DFBPPR17448 ---- Animal proteins ---- Gap junction alpha-1 protein Source.13: DFBPPR18548 ---- Animal proteins ---- Lipoyl synthase, mitochondrial Source.14: DFBPPR18804 ---- Animal proteins ---- Importin subunit alpha-8 Source.15: DFBPPR19039 ---- Animal proteins ---- Signal peptidase complex catalytic subunit SEC11A Source.16: DFBPPR19506 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.17: DFBPPR21259 ---- Animal proteins ---- Elongation factor 1-delta Source.18: DFBPPR21316 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit alpha Source.19: DFBPPR21564 ---- Animal proteins ---- HORMA domain-containing protein 2 Source.20: DFBPPR22593 ---- Animal proteins ---- UPF0688 protein C1orf174 homolog Source.21: DFBPPR8757 ---- Animal proteins ---- Gap junction alpha-1 protein Source.22: DFBPPR8902 ---- Animal proteins ---- Cytochrome P450 2E1 Source.23: DFBPPR8984 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.24: DFBPPR9625 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.25: DFBPPR9634 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.26: DFBPPR9783 ---- Animal proteins ---- Importin subunit alpha-8 Source.27: DFBPPR10690 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.28: DFBPPR11024 ---- Animal proteins ---- Gap junction alpha-1 protein Source.29: DFBPPR11999 ---- Animal proteins ---- Dymeclin Source.30: DFBPPR12108 ---- Animal proteins ---- Protein strawberry notch homolog 1 Source.31: DFBPPR12353 ---- Animal proteins ---- Cytochrome P450 2E1 Source.32: DFBPPR12399 ---- Animal proteins ---- Gap junction alpha-1 protein Source.33: DFBPPR12494 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.34: DFBPPR12717 ---- Animal proteins ---- Phosphorylase b kinase regulatory subunit alpha, liver isoform Source.35: DFBPPR13047 ---- Animal proteins ---- Elongation factor 1-delta Source.36: DFBPPR13058 ---- Animal proteins ---- Anion exchange transporter Source.37: DFBPPR13961 ---- Animal proteins ---- Elongation factor 1-delta Source.38: DFBPPR14891 ---- Microorganism protein ---- DNA polymerase epsilon catalytic subunit A Source.39: DFBPPR14966 ---- Microorganism protein ---- PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Source.40: DFBPPR15567 ---- Microorganism protein ---- Mating-type protein A2
Link-research Inductive analysis	Link 1: DFBPACEI1328----Laminaria japonica----Laminaria japonica hydrolysates

Biological/Functional activity & target protein

ACE-inhibitory activity	N.D
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Enzyme ratio (papain: alcalase: trypsin) 1:2:1, pH 8.0, temperature 60 degrees C, substrate concentration 1.7%, enzyme addition 4.4%, and hydrolysis time 5.1 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

These results demonstrated the potential applications of LJPs as a source of anti-hypertensive peptides and provided a reference for the industrialized anti-hypertensive productions.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Kumagai Y, Toji K, Katsukura S, Morikawa R, Uji T, Yasui H, Shimizu T, Kishimura H. Characterization of ACE Inhibitory Peptides Prepared from Pyropia pseudolinearis Protein. Mar Drugs. 2021 Apr 1;19(4):200. PMID: PMC8066288 DOI: 10.3390/md19040200
Other literature(s)	N.D
PubDate	2021