Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2219(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2219
Peptide sequence	SKTY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ser-Lys-Thr-Tyr

Single-letter amino acid

SKTY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	497.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.70 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.6750^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant, Marine
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica proteins (LJPs)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0748 ---- Plant proteins ---- Agglutinin Source.2: DFBPPR1279 ---- Plant proteins ---- Homeobox protein HAZ1 Source.3: DFBPPR1565 ---- Plant proteins ---- Nuclear cap-binding protein subunit 1 Source.4: DFBPPR1751 ---- Plant proteins ---- Heat stress transcription factor A-4b Source.5: DFBPPR1919 ---- Plant proteins ---- Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial Source.6: DFBPPR2093 ---- Plant proteins ---- Ent-kaurene oxidase-like 5 Source.7: DFBPPR2310 ---- Plant proteins ---- Heat stress transcription factor A-3 Source.8: DFBPPR2472 ---- Plant proteins ---- Heat stress transcription factor B-4d Source.9: DFBPPR2847 ---- Plant proteins ---- Glutaredoxin-C4, chloroplastic Source.10: DFBPPR3108 ---- Plant proteins ---- Thioredoxin H4-2 Source.11: DFBPPR3186 ---- Plant proteins ---- Endoglucanase 10 Source.12: DFBPPR3471 ---- Plant proteins ---- Monothiol glutaredoxin-S6 Source.13: DFBPPR3775 ---- Plant proteins ---- Kinesin-like protein KIN-14G Source.14: DFBPPR3996 ---- Plant proteins ---- Kinesin-like protein KIN-14J Source.15: DFBPPR5020 ---- Plant proteins ---- Basic 7S globulin Source.16: DFBPPR5046 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 2 Source.17: DFBPPR5049 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 1 Source.18: DFBPPR5140 ---- Plant proteins ---- Basic 7S globulin 2 Source.19: DFBPPR5836 ---- Plant proteins ---- Eukaryotic translation initiation factor 5A Source.20: DFBPPR6469 ---- Plant proteins ---- Oxygen-evolving enhancer protein 1, chloroplastic Source.21: DFBPPR6889 ---- Plant proteins ---- Oxygen-evolving enhancer protein 1, chloroplastic Source.22: DFBPPR7465 ---- Plant proteins ---- Oleosin S1-2 Source.23: DFBPPR15974 ---- Animal proteins ---- Androgen receptor Source.24: DFBPPR15985 ---- Animal proteins ---- von Willebrand factor Source.25: DFBPPR16242 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Source.26: DFBPPR16320 ---- Animal proteins ---- Guanylate cyclase soluble subunit beta-1 Source.27: DFBPPR16942 ---- Animal proteins ---- Alkaline phosphatase, tissue-nonspecific isozyme Source.28: DFBPPR17121 ---- Animal proteins ---- 3-hydroxyacyl-CoA dehydrogenase type-2 Source.29: DFBPPR17188 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Source.30: DFBPPR17439 ---- Animal proteins ---- Folliculin Source.31: DFBPPR17745 ---- Animal proteins ---- N-lysine methyltransferase KMT5A Source.32: DFBPPR17804 ---- Animal proteins ---- Calpain-1 catalytic subunit Source.33: DFBPPR17891 ---- Animal proteins ---- Intestinal-type alkaline phosphatase Source.34: DFBPPR18096 ---- Animal proteins ---- Serine palmitoyltransferase 1 Source.35: DFBPPR19040 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 11 Source.36: DFBPPR19068 ---- Animal proteins ---- CXXC-type zinc finger protein 1 Source.37: DFBPPR19956 ---- Animal proteins ---- Cysteine and histidine-rich domain-containing protein 1 Source.38: DFBPPR20434 ---- Animal proteins ---- SPRY domain-containing SOCS box protein 1 Source.39: DFBPPR21186 ---- Animal proteins ---- 40S ribosomal protein S2 Source.40: DFBPPR21545 ---- Animal proteins ---- Musculoskeletal embryonic nuclear protein 1 Source.41: DFBPPR22666 ---- Animal proteins ---- Uncharacterized protein C12orf50 homolog Source.42: DFBPPR8867 ---- Animal proteins ---- Calpain-1 catalytic subunit Source.43: DFBPPR9344 ---- Animal proteins ---- Desmoglein-1 Source.44: DFBPPR9555 ---- Animal proteins ---- Cysteine and histidine-rich domain-containing protein 1 Source.45: DFBPPR10104 ---- Animal proteins ---- Bloom syndrome protein homolog Source.46: DFBPPR10142 ---- Animal proteins ---- Calpain-3 Source.47: DFBPPR10363 ---- Animal proteins ---- E3 ubiquitin-protein ligase HACE1 Source.48: DFBPPR10608 ---- Animal proteins ---- Semaphorin-3D Source.49: DFBPPR10636 ---- Animal proteins ---- LARGE xylosyl- and glucuronyltransferase 1 Source.50: DFBPPR11774 ---- Animal proteins ---- Musculoskeletal embryonic nuclear protein 1 Source.51: DFBPPR11933 ---- Animal proteins ---- M-protein, striated muscle Source.52: DFBPPR12004 ---- Animal proteins ---- Fibronectin type-III domain-containing protein 3a Source.53: DFBPPR12071 ---- Animal proteins ---- Cysteine and histidine-rich domain-containing protein 1 Source.54: DFBPPR12475 ---- Animal proteins ---- Potassium-transporting ATPase subunit beta Source.55: DFBPPR14555 ---- Marine protein ---- Vitellogenin Source.56: DFBPPR15377 ---- Microorganism protein ---- Mitochondrial presequence protease Source.57: DFBPPR15570 ---- Microorganism protein ---- Glucose starvation modulator protein 1 Source.58: DFBPPR8239 ---- Plant protein ---- Serine--tRNA ligase Source.59: DFBPPR8279 ---- Plant protein ---- Oxygen-evolving enhancer protein 1, chloroplastic
Link-research Inductive analysis	Link 1: DFBPACEI1323----Laminaria japonica----Laminaria japonica hydrolysates

Biological/Functional activity & target protein

ACE-inhibitory activity	N.D
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Enzyme ratio (papain: alcalase: trypsin) 1:2:1, pH 8.0, temperature 60 degrees C, substrate concentration 1.7%, enzyme addition 4.4%, and hydrolysis time 5.1 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

These results demonstrated the potential applications of LJPs as a source of anti-hypertensive peptides and provided a reference for the industrialized anti-hypertensive productions.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Kumagai Y, Toji K, Katsukura S, Morikawa R, Uji T, Yasui H, Shimizu T, Kishimura H. Characterization of ACE Inhibitory Peptides Prepared from Pyropia pseudolinearis Protein. Mar Drugs. 2021 Apr 1;19(4):200. PMID: PMC8066288 DOI: 10.3390/md19040200
Other literature(s)	N.D
PubDate	2021