Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2220(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2220
Peptide sequence	AKYSY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ala-Lys-Tyr-Ser-Tyr

Single-letter amino acid

AKYSY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	630.69 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.52 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.1000^c

Hydrophilic residue ratio

20%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant, Marine
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica proteins (LJPs)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1035 ---- Plant proteins ---- Probable L-ascorbate peroxidase 8, chloroplastic Source.2: DFBPPR1480 ---- Plant proteins ---- CASP-like protein BLE3 Source.3: DFBPPR5989 ---- Plant proteins ---- CASP-like protein 1C2 Source.4: DFBPPR14346 ---- Marine protein ---- R-phycoerythrin alpha chain Source.5: DFBPPR7860 ---- Plant protein ---- CASP-like protein 1C1
Link-research Inductive analysis	Link 1: DFBPACEI0786----Red algae----Red algae protein Link 2: DFBPACEI1325----Laminaria japonica----Laminaria japonica hydrolysates

Biological/Functional activity & target protein

ACE-inhibitory activity	N.D
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Enzyme ratio (papain: alcalase: trypsin) 1:2:1, pH 8.0, temperature 60 degrees C, substrate concentration 1.7%, enzyme addition 4.4%, and hydrolysis time 5.1 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

These results demonstrated the potential applications of LJPs as a source of anti-hypertensive peptides and provided a reference for the industrialized anti-hypertensive productions.

Database cross-references

DFBP

[D1]	DFBPANHY0661
[D2]	DFBPMUFU0173

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Kumagai Y, Toji K, Katsukura S, Morikawa R, Uji T, Yasui H, Shimizu T, Kishimura H. Characterization of ACE Inhibitory Peptides Prepared from Pyropia pseudolinearis Protein. Mar Drugs. 2021 Apr 1;19(4):200. PMID: PMC8066288 DOI: 10.3390/md19040200
Other literature(s)	N.D
PubDate	2021