Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2231(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2231
Peptide sequence	ASGLCPEEAVPRR
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide, BP1

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], DPP IV-inhibitory activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Ala-Ser-Gly-Leu-Cys-Pro-Glu-Glu-Ala-Val-Pro-Arg-Arg

Single-letter amino acid

ASGLCPEEAVPRR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
1384.56 Da	1384.56 Da ^c

Net charge

-0.1

Isoelectric point (pI)

6.45 ^c

IC₅₀

59.90 ± 9.52 μg/mL (43.40 μM)

pIC₅₀

-1.777

GRAVY

-0.4846^c

Hydrophilic residue ratio

53.85%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Picrorhiza kurroa Royle ex Benth.
Precursor protein	Defatted leaf tissue
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 59.90 ± 9.52 μg/mL (43.40 μM).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	P. kurroa protein isolate was enzymatically hydrolyzed using trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

BP1 has antioxidant potential and ACE and DPP-IV inhibitory activities that could be used for peptide-based formulation(s) in pharmaceuticals to treat diabetes, cardiovascular diseases, and other diseases associated with ROS.

Database cross-references

DFBP

[D1]	DFBPANOX1201
[D2]	DFBPDPIV0236
[D3]	DFBPMUFU0641

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Thakur S, Chhimwal J, Joshi R, Kumari M, Padwad Y, Kumar R. Evaluating Peptides of Picrorhiza kurroa and Their Inhibitory Potential against ACE, DPP-IV, and Oxidative Stress. J Proteome Res. 2021 Aug 6;20(8):3798-3813. PMID: 34254800 DOI: 10.1021/acs.jproteome.1c00081
Other literature(s)	N.D
PubDate	2021