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List of peptide properties
DFBP ID - DFBPACEI2235(ACE-inhibitory peptide)
DFBP ID DFBPACEI2235
Peptide sequence SSAPMF
Type Native peptide
Peptide/Function name ACE-inhibitory peptide
Function-activity relationship
Main bioactivity ACE-inhibitory activity
Otheir bioactivity N.D
Calculated physicochemical properties
Three-letter amino acid Ser-Ser-Ala-Pro-Met-Phe
Single-letter amino acid SSAPMF
Peptide length 6
Peptide mass
Experimental mass Theoretical mass
N.D 638.72 Da c
Net charge 0.00 c
Isoelectric point (pI) 5.97 c
IC50 32.94 µM
pIC50 -1.518
GRAVY 0.5500 c
Hydrophilic residue ratio 66.67% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Microorganism
Organism/Source Yeast
Precursor protein Yeast powder
Residue position N.D
Precursor protein(s) search
No matching precursor protein found
Link-research
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
ACE-inhibitory activity

(1) The peptide SSAPMF exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity an IC50 value of 32.94 µM.
(2) Molecular docking analysis indicated that the SSAPMF was mainly bound in the S1(Ala354) (PDB ID: 1O8A).

Specific target protein(s) Specific Target Protein(s):
Angiotensin-converting enzyme
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Non-bitter taste prediction
SMILES N.D
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

Yeast powder was mixed with crude enzyme (derived from B. subtilis) and distilled water at an enzyme/substrate ratio (E/S) of 7000 U/g. The hydrolysis was performed at 55 °C for 5 h.

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
BIOPEP-UWM [D1] -
APD [D2] -
BioPepDB [D3] -
MBPDB [D4] -
Reference(s)
Primary literature Huang Y, Jia F, Zhao J, Hou Y, Hu SQ. Novel ACE Inhibitory Peptides Derived from Yeast Hydrolysates: Screening, Inhibition Mechanisms and Effects on HUVECs. J Agric Food Chem. 2021 Mar 3;69(8):2412-2421.
PMID: 33593053
Other literature(s) N.D
PubDate 2021
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214