Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2244(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2244
Peptide sequence	RYFPT
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Arg-Tyr-Phe-Pro-Thr

Single-letter amino acid

RYFPT

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	682.77 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.82 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.0600^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Microorganism
Organism/Source	Yeast
Precursor protein	Yeast powder
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1646 ---- Plant proteins ---- ADP,ATP carrier protein, mitochondrial Source.2: DFBPPR5618 ---- Plant proteins ---- ADP,ATP carrier protein 2, mitochondrial Source.3: DFBPPR5619 ---- Plant proteins ---- ADP,ATP carrier protein 1, mitochondrial Source.4: DFBPPR6757 ---- Plant proteins ---- ADP,ATP carrier protein 2, mitochondrial Source.5: DFBPPR6758 ---- Plant proteins ---- ADP,ATP carrier protein 1, mitochondrial Source.6: DFBPPR17512 ---- Animal proteins ---- ADP/ATP translocase 1 Source.7: DFBPPR18124 ---- Animal proteins ---- ADP/ATP translocase 3 Source.8: DFBPPR18450 ---- Animal proteins ---- ADP/ATP translocase 2 Source.9: DFBPPR20031 ---- Animal proteins ---- ADP/ATP translocase 4 Source.10: DFBPPR8830 ---- Animal proteins ---- Electrogenic sodium bicarbonate cotransporter 1 Source.11: DFBPPR9260 ---- Animal proteins ---- ADP/ATP translocase 3 Source.12: DFBPPR10415 ---- Animal proteins ---- Semaphorin-3A Source.13: DFBPPR12458 ---- Animal proteins ---- Electrogenic sodium bicarbonate cotransporter 1 Source.14: DFBPPR12650 ---- Animal proteins ---- ADP/ATP translocase 1 Source.15: DFBPPR15023 ---- Microorganism protein ---- ADP,ATP carrier protein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	(1) The peptides RYFPT exhibited poor Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity of 17.47 ± 2.45% at a concentration of 188 μg/mL. Thus, the IC₅₀ values of this peptide was not determined. (2) Molecular docking analysis indicated that the RYFPT was mainly bound to Gln281, Ala354 (PDB ID: 1O8A).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Yeast powder was mixed with crude enzyme (derived from B. subtilis) and distilled water at an enzyme/substrate ratio (E/S) of 7000 U/g. The hydrolysis was performed at 55 °C for 5 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Huang Y, Jia F, Zhao J, Hou Y, Hu SQ. Novel ACE Inhibitory Peptides Derived from Yeast Hydrolysates: Screening, Inhibition Mechanisms and Effects on HUVECs. J Agric Food Chem. 2021 Mar 3;69(8):2412-2421. PMID: 33593053 DOI: 10.1021/acs.jafc.0c06053
Other literature(s)	N.D
PubDate	2021