Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2255(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2255
Peptide sequence	DNLLN
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Asp-Asn-Leu-Leu-Asn

Single-letter amino acid

DNLLN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	587.62 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.13 ^c

IC₅₀

235 uM

pIC₅₀

-2.371

GRAVY

-0.5800^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant, Food
Organism/Source	Pixian broad bean paste (PBBP)
Precursor protein	The water-soluble peptide extracts of PBBP
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2876 ---- Plant proteins ---- Kinesin-like protein KIN-5A Source.2: DFBPPR3512 ---- Plant proteins ---- Probable protein phosphatase 2C 3 Source.3: DFBPPR7466 ---- Plant proteins ---- 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic Source.4: DFBPPR8190 ---- Plant proteins ---- Acyl-coenzyme A oxidase, peroxisomal Source.5: DFBPPR17264 ---- Animal proteins ---- Tubulin-specific chaperone D Source.6: DFBPPR17540 ---- Animal proteins ---- Breast cancer type 1 susceptibility protein homolog Source.7: DFBPPR18005 ---- Animal proteins ---- Lutropin-choriogonadotropic hormone receptor Source.8: DFBPPR21455 ---- Animal proteins ---- Apolipoprotein C-IV Source.9: DFBPPR8767 ---- Animal proteins ---- Lutropin-choriogonadotropic hormone receptor Source.10: DFBPPR10524 ---- Animal proteins ---- Protein disulfide-isomerase Source.11: DFBPPR10753 ---- Animal proteins ---- Hypermethylated in cancer 1 protein Source.12: DFBPPR14422 ---- Marine protein ---- Translation initiation factor IF-2, chloroplastic Source.13: DFBPPR15719 ---- Microorganism protein ---- Enhancer of translation termination 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide DNLLN exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₂₀₀ value of 235 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Pixian broad bean paste employed in this study was provided by Sichuan Pixian Douban Co., Ltd. (Pixian, Sichuan, China) and held at −20 ℃.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX1203
[D2]	DFBPMUFU0653

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Li, M., Fan, W. and Xu, Y. (2021) Identification of angiotensin converting enzyme (ACE) inhibitory and antioxidant peptides derived from Pixian broad bean paste. LWT, 151, 112221. DOI: 10.1016/j.lwt.2021.112221
Other literature(s)	N.D
PubDate	2021