Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPALGL0011(α-Glucosidase inhibitory peptide)

DFBP ID	DFBPALGL0011
Peptide sequence	KLPGF
Type	Native peptide
Peptide/Function name	α-Glucosidase inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	α-Glucosidase inhibitory activity
Otheir bioactivity	α-Amylase inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Lys-Leu-Pro-Gly-Phe

Single-letter amino acid

KLPGF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
560.3640 Da	560.69 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.1400^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg white protein
Precursor protein	Albumin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1630 ---- Plant proteins ---- Exportin-T Source.2: DFBPPR17993 ---- Animal proteins ---- Ubiquitin-like modifier-activating enzyme 1 Source.3: DFBPPR18423 ---- Animal proteins ---- Bile acid receptor Source.4: DFBPPR20416 ---- Animal proteins ---- Interphotoreceptor matrix proteoglycan 1 Source.5: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Source.6: DFBPPR10332 ---- Animal proteins ---- Interphotoreceptor matrix proteoglycan 1 Source.7: DFBPPR12544 ---- Animal proteins ---- Ubiquitin-like modifier-activating enzyme 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

α-Glucosidase inhibitory activity

Inhibitor of α-glucosidase (EC 3.2.1.20). The peptide KLPGF had α-glucosidase inhibitory activity with an IC₅₀ of 59.5 ± 5.7 umol/l and α-amylase inhibitory activity with an IC50 of 120.0 ± 4.0 umol/l, the results revealed that the peptide KLPGF was a potential anti-diabetic inhibitor. The α-glucosidase inhibitory activity of KLPGF was comparable to that of the therapeutic AGI drug acarbose (IC₅₀ = 60.8 umol/l) ^[1].

Table 1 α-Glucosidase and α-amylase inhibitory activities of the peptides
Peptide sequences	α-Glucosidase inhibitory activity (IC₅₀)	α-amylase inhibitory activity (IC₅₀)
KLPGF	59.5 ± 5.7 μmol/l	120.0 ± 4.0 μmol/l
EVSGL	> 150.0 μmol/l	> 150.0 μmol/l
QITKPN	> 150.0 μmol/l	> 150.0 μmol/l
AEAGVD	> 150.0 μmol/l	> 150.0 μmol/l
EAGVD	> 150.0 μmol/l	110.0 ± 6.2 μmol/l
NVLQPS	100.0 ± 5.7 μmol/l	> 150.0 μmol/l
LEPINF	> 150.0 μmol/l	> 150.0 μmol/l
ANENIF	> 150.0 μmol/l	> 150.0 μmol/l

Specific target protein(s)

Specific Target Protein(s):
Lysosomal alpha-glucosidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The alkaline proteinase Alcalase was added at 4.0% of protein (w/w) for digestion.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPALAM0004
[D2]	DFBPMUFU0743

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Yu Z, Yin Y, Zhao W, Liu J, Chen F. Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase. Food Chem. 2012 Dec 1;135(3):2078-85. PMID: 22953959 DOI: 10.1016/j.foodchem.2012.06.088
Other literature(s)	[1] Seo W D, Kim J H, Kang J E, et al. Sulfonamide chalcone as a new class of alpha-glucosidase inhibitors[J]. Bioorganic & Medicinal Chemistry Letters, 2005, 15(24):5514-5516.
PubDate	2012