Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPALGL0027(α-Glucosidase inhibitory peptide)

DFBP ID	DFBPALGL0027
Peptide sequence	YINQMPQKSRE
Type	Native peptide
Peptide/Function name	α-Glucosidase inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	α-Glucosidase inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], DPP IV-inhibitory activity ^[D3], Multifunctional activity ^[D4]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Ile-Asn-Gln-Met-Pro-Gln-Lys-Ser-Arg-Glu

Single-letter amino acid

YINQMPQKSRE

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1393.58 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.70 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.7909^c

Hydrophilic residue ratio

27.27%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg
Precursor protein	Egg yolk protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR11267 ---- Animal proteins ---- Apolipoprotein B
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

α-Glucosidase inhibitory activity	The peptide exhibited very weak α-Glucosidase (AGI, EC 3.2.1.20) inhibitory activity with an IC₅₀ value of 1694.3 ug/mL.
Specific target protein(s)	Specific Target Protein(s): Lysosomal alpha-glucosidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCSC)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The egg yolk protein by-product following ethanol extraction of phospholipids (YP) was hydrolyzed with Pepsin (EC. 3.4.23.1) to produce and identify novel peptides.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1773
[D2]	DFBPANOX0743
[D3]	DFBPDPIV0094
[D4]	DFBPMUFU0554

BIOPEP-UWM

[D5]

8739, 8742, 8747

APD

[D6]

BioPepDB

[D7]

MBPDB

[D8]

Reference(s)

Primary literature	Zambrowicz A, Pokora M, Setner B, Dąbrowska A, Szołtysik M, Babij K, Szewczuk Z, Trziszka T, Lubec G, Chrzanowska J. Multifunctional peptides derived from an egg yolk protein hydrolysate: isolation and characterization. Amino Acids. 2015 Feb;47(2):369-80. PMID: 25408464 DOI: 10.1007/s00726-014-1869-x
Other literature(s)	N.D
PubDate	2015