Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0054(Antimicrobial peptide)

DFBP ID	DFBPAMIC0054
Peptide sequence	GICACRRRFCPNSERFSGYCRVNGARYVRCCSRR
Type	Native peptide
Peptide/Function name	Antimicrobial peptide, Rabbit neutrophil defensin 3a

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	Opioid activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Gly-Ile-Cys-Ala-Cys-Arg-Arg-Arg-Phe-Cys-Pro-Asn-Ser-Glu-Arg-Phe-Ser-Gly-Tyr-Cys-Arg-Val-Asn-Gly-Ala-Arg-Tyr-Val-Arg-Cys-Cys-Ser-Arg-Arg

Single-letter amino acid

GICACRRRFCPNSERFSGYCRVNGARYVRCCSRR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
3993.00 Da	4003.65 Da ^c

Net charge

Isoelectric point (pI)

10.25 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6382^c

Hydrophilic residue ratio

32.35%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Rabbit, Oryctolagus cuniculus
Precursor protein	Peritoneal neutrophil protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR12880 ---- Animal proteins ---- Corticostatin 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity	anti-Gram+ & Gram-, antiviral, antifungal.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CS)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CO)C(=O)NCC(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Extract without enzyme
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	Neutrophil peptide was reduced and carboxymethylated, or performic acid-oxidizedprior t o digestion with trypsin,a-chymotrypsin, or S. aureus V protease.
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPOPIO0070
[D2]	DFBPMUFU0700

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Selsted ME, Brown DM, DeLange RJ, Harwig SS, Lehrer RI. Primary structures of six antimicrobial peptides of rabbit peritoneal neutrophils. J Biol Chem. 1985 Apr 25;260(8):4579-84. PMID: 3988726
Other literature(s)	[1] Selsted M E, Brown D M, Delange R J, et al. Primary structures of MCP-1 and MCP-2, natural peptide antibiotics of rabbit lung macrophages[J]. Journal of Biological Chemistry, 1983, 258(23):14485-9.
PubDate	1985