Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0171(Antimicrobial peptide)

DFBP ID	DFBPAMIC0171
Peptide sequence	GWGSFFKKAAHVGKHVGKAALTHYL
Type	Native peptide
Peptide/Function name	Antimicrobial peptide, NRC-4

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	Anticancer activity ^[D1], Haemolytic activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Gly-Trp-Gly-Ser-Phe-Phe-Lys-Lys-Ala-Ala-His-Val-Gly-Lys-His-Val-Gly-Lys-Ala-Ala-Leu-Thr-His-Tyr-Leu

Single-letter amino acid

GWGSFFKKAAHVGKHVGKAALTHYL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
2711.00 Da	2711.16 Da ^c

Net charge

+6.5

Isoelectric point (pI)

10.64 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.0680^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	winter flounder 2 2.1, Pleuronectes americanus
Precursor protein	Flatfish
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	Link 1: DFBPAMIC0033----Winter flounder, Pleuronectes americanus----The skin mucous secretions

Biological/Functional activity & target protein

Antimicrobial activity

(1) The effective active sequence of the peptide is GWGSFFKKAAHVGKHVGKAALTHYL-NH₂;
(2) anti-Gram+ & Gram-, antifungal;
(3) Highly active against A. salmonicida 99-1, A. salmonicida 97-4, S. enterica serovar Typhimurium MS7953s, S. enterica serovar Typhimurium 14028s, P. aeruginosa K799, P. aeruginosa Z61, E. coli CGSC4908, E. coli UB1005, E. coli DC2, S. epidermidis C621, MRSA C623, C. albicans C627 (MIC 2-16 uM).
(4) MIC value = 2.5-5 uM ^[1].

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)NCC(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANCA0657, DFBPANCA0658
[D2]	DFBPHAPE0022, DFBPHAPE0023
[D3]	DFBPMUFU0699

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Patrzykat A, Gallant JW, Seo JK, Pytyck J, Douglas SE. Novel antimicrobial peptides derived from flatfish genes. Antimicrob Agents Chemother. 2003 Aug;47(8):2464-70. PMID: 12878506 DOI: 10.1128/aac.47.8.2464-2470.2003
Other literature(s)	[1] Sung W S, Lee D G. Pleurocidin-derived antifungal peptides with selective membrane-disruption effect[J]. Biochemical & Biophysical Research Communications, 2008, 369(3):858-861.
PubDate	2003