Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0008(Antithrombotic peptide)

DFBP ID	DFBPANTH0008
Peptide sequence	GPRPP
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Pro-Arg-Pro-Pro

Single-letter amino acid

GPRPP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	522.60 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.9400^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig
Precursor protein	Porcine skin collagen
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR4863 ---- Plant proteins ---- Protein MOTHER of FT and TFL1 homolog 1 Source.2: DFBPPR17156 ---- Animal proteins ---- ATP-dependent RNA helicase A Source.3: DFBPPR17644 ---- Animal proteins ---- CCAAT/enhancer-binding protein beta Source.4: DFBPPR20083 ---- Animal proteins ---- GPN-loop GTPase 1
Link-research Inductive analysis	Link 1: DFBPANTH0067----Pig----Porcine skin collagen Link 2: DFBPANTH0081----Pig----Porcine skin collagen

Biological/Functional activity & target protein

Antithrombotic activity

GPRPP highly inhibited fibrinogen/thrombin clotting, but did not inhibit the activity of thrombin. GPRPP-NH₂ is 4.6-times more potent than that of GPRP^[2].

Table 1 Inhibitory effects of collagen-related synthetic peptides on fibrinogen / thrombin clotting
Peptide	IC50 (μM)	References
Gly-Pro-Arg	250	[1]
Gly-Pro-Arg-Pro	110	[1]
Gly-Pro-Arg-Gly	510	-
Gly-Pro-Arg-Gly-Pro	380
Gly-Pro-Arg-Gly-Pro-Ala	260
Gly-Pro-Arg-Gly-Pro-Pro	230
Gly-Pro-Arg-Pro-Pro	50
Gly-Pro-Arg-Gly-Pro-NH₂	40	[2]

Specific target protein(s)

Potential Target Protein(s):
Fibrinogen gamma chain
Fibrinogen alpha chain
Fibrinogen beta chain
Fibroleukin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthetic peptide
Enzyme(s)/starter culture	The peptide was synthesized by the solid-phase method with a 430A Peptide Synthesizer (Applied Biosystems).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Maruyama, S., Nonaka, I., Tanaka, H. Inhibitory effects of enzymatic hydrolysates of collagen and collagen-related synthetic peptides on fibrinogen/thrombin clotting. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1993, 1164, 215-8. DOI: 10.1016/0167-4838(93)90250-U
Other literature(s)	[1] Laudano A P, Doolittle R F. Studies on synthetic peptides that bind to fibrinogen and prevent fibrin polymerization. Structural requirements, number of binding sites, and species differences.[J]. Biochemistry, 1980, 19(5):1013-1019. [2] Kawasaki K, Hirase K, Tsuji T, et al. Amino acids and peptides XII: synthetic peptides related to the N-terminal portion of fibrin alpha-chain and their inhibitory effects on fibrinogen/thrombin clotting.[J]. Thrombosis Research, 1989, 56(6):757-762.
PubDate	1993