Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0026(Antithrombotic peptide)

DFBP ID	DFBPANTH0026
Peptide sequence	VTRGD
Type	Native peptide
Peptide/Function name	Antithrombotic peptide, Fibronectin inhibitor

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-Thr-Arg-Gly-Asp

Single-letter amino acid

VTRGD

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	546.58 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.74 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9800^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine
Precursor protein	Fibronectin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1963 ---- Plant proteins ---- Potassium channel AKT1 Source.2: DFBPPR3103 ---- Plant proteins ---- Beta-glucosidase 4 Source.3: DFBPPR8428 ---- Plant proteins ---- 11S globulin seed storage protein 2 Source.4: DFBPPR9073 ---- Animal proteins ---- Vitronectin Source.5: DFBPPR12408 ---- Animal proteins ---- Vitronectin Source.6: DFBPPR12743 ---- Animal proteins ---- Katanin p60 ATPase-containing subunit A-like 1 Source.7: DFBPPR13388 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 6
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity	The peptide showed promoting cell attachment activity.
Specific target protein(s)	Potential Target Protein(s): Fibrinogen gamma chain Fibrinogen alpha chain Fibrinogen beta chain Fibroleukin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)NCC(=O)N[C@@]([H])(CC(=O)O)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Pierschbacher MD, Ruoslahti E. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature. 1984 May 3-9;309(5963):30-3. PMID: 6325925 DOI: 10.1038/309030a0
Other literature(s)	N.D
PubDate	1984