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List of peptide properties
DFBP ID - DFBPANTH0084(Antithrombotic peptide)
DFBP ID DFBPANTH0084
Peptide sequence QIGLF
Type Native peptide
Peptide/Function name Antithrombotic peptide
Function-activity relationship
Main bioactivity Antithrombotic activity
Otheir bioactivity ACE-inhibitory activity [D1], Antioxidative activity [D2], α-Amylase inhibitory activity [D3], α-Glucosidase inhibitory activity [D4], Multifunctional activity [D5]
Calculated physicochemical properties
Three-letter amino acid Gln-Ile-Gly-Leu-Phe
Single-letter amino acid QIGLF
Peptide length 5
Peptide mass
Experimental mass Theoretical mass
N.D 576.69 Da c
Net charge 0.00 c
Isoelectric point (pI) 5.97 c
IC50 N.D
pIC50 N.D
GRAVY 1.4400 c
Hydrophilic residue ratio 80% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Animal
Organism/Source Egg white protein
Precursor protein Ovotransferrin
Residue position N.D
Precursor protein(s) search
Link-research
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
Antithrombotic activity
Peptide
Anticoagulation activity (%)
0.01 (mM)
0.1 (mM)
1 (mM)
100 (mM)
QIGLF1.0 ± 0.6
0.9 ± 0.6
1.2 ± 0.8
5.9 ± 0.5
Specific target protein(s) Specific Target Protein(s):
Prothrombin
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

Hydrolysis with alkaline proteinase Alcalase.

Stability & Cytotoxicity
Peptide stability
Literature report:

The result of simulated gastrointestinal digestion by pepsin and trypsin showed that QIGLF had a strong resistance to gastrointestinal proteases, providing a possibility for oral administration [1].

EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report:

QIGLF was added to Caco-2 cells followed by addition of H2O2, and exhibited significant cytoprotective effect in Caco-2 cells against oxidative stress induced by H2O2 [1].

Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
DFBP
[D1] DFBPACEI0160
[D2] DFBPANOX0620
[D3] DFBPALAM0019
[D4] DFBPALGL0019
[D5] DFBPMUFU0063
BIOPEP-UWM [D6] 8395, 9411
APD [D7] -
BioPepDB [D8] -
MBPDB [D9] -
Reference(s)
Primary literature Yu Z, Liu B, Zhao W, Yin Y, Liu J, Chen F. Primary and secondary structure of novel ACE-inhibitory peptides from egg white protein. Food Chem. 2012 Jul 15;133(2):315-22.
PMID: 25683401
Other literature(s)

[1] Ding L, Zhang Y, Jiang Y, et al. Transport of Egg White ACE-Inhibitory Peptide, Gln-Ile-Gly-Leu-Phe, in Human Intestinal Caco-2 Cell Monolayers with Cytoprotective Effect.[J]. Journal of Agricultural (&) Food Chemistry, 2014, 62(14):3177-3182.

PubDate 2012
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214