DFBP ID - DFBPANTH0085(Antithrombotic peptide)

DFBP ID DFBPANTH0085 Peptide sequence RVPSLM Type Native peptide Peptide/Function name Antithrombotic peptide

Function-activity relationship

Main bioactivity Antithrombotic activity Otheir bioactivity Antioxidative activity [D1], α-Amylase inhibitory activity [D2], α-Glucosidase inhibitory activity [D3], Multifunctional activity [D4]

Calculated physicochemical properties

Three-letter amino acid Arg-Val-Pro-Ser-Leu-Met Single-letter amino acid RVPSLM Peptide length 6 Peptide mass Experimental mass Theoretical mass N.D 701.87 Da c Net charge 0.00 c Isoelectric point (pI) 11.04 c IC50 N.D pIC50 N.D GRAVY 0.5000 c Hydrophilic residue ratio 66.67% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Egg white protein Precursor protein Ovotransferrin Residue position N.D Precursor protein(s) search Source.1: DFBPPR9971 ---- Animal proteins ---- Ovotransferrin Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity Peptide Anticoagulation activity (%) 0.01 (mM) 0.1 (mM) 1 (mM) 100 (mM) RVPSLM 1.1 ± 0.4 1.0 ± 0.3 2.6 ± 0.5 13.0 ± 5.9 Specific target protein(s) Specific Target Protein(s): Prothrombin

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Bitter taste prediction SMILES N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCSC)C(=O)O

Preparation method

Mode of preparation Enzymatic hydrolysis Enzyme(s)/starter culture Hydrolysis with alkaline proteinase Alcalase.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

DFBP [D1] DFBPANOX0621 [D2] DFBPALAM0012 [D3] DFBPALGL0003 [D4] DFBPMUFU0671

BIOPEP-UWM [D5] 9389

APD [D6] -

BioPepDB [D7] -

MBPDB [D8] -

Reference(s)

Primary literature Yu Z, Liu B, Zhao W, Yin Y, Liu J, Chen F. Primary and secondary structure of novel ACE-inhibitory peptides from egg white protein. Food Chem. 2012 Jul 15;133(2):315-22. PMID: 25683401 DOI: 10.1016/j.foodchem.2012.01.032 Other literature(s) N.D PubDate 2012