Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0087(Antithrombotic peptide)

DFBP ID	DFBPANTH0087
Peptide sequence	DLQGK
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	Antioxidative activity ^[D1], α-Amylase inhibitory activity ^[D2], α-Glucosidase inhibitory activity ^[D3], Multifunctional activity ^[D4]

Calculated physicochemical properties

Three-letter amino acid

Asp-Leu-Gln-Gly-Lys

Single-letter amino acid

DLQGK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	559.62 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.71 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.5000^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg white protein
Precursor protein	Ovotransferrin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR3587 ---- Plant proteins ---- Auxin transport protein BIG Source.2: DFBPPR4464 ---- Plant proteins ---- CASP-like protein 4B4 Source.3: DFBPPR21246 ---- Animal proteins ---- Dynein intermediate chain CFAP94, axonemal Source.4: DFBPPR9112 ---- Animal proteins ---- Calcium-activated chloride channel regulator 1 Source.5: DFBPPR9971 ---- Animal proteins ---- Ovotransferrin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity

Peptide	Anticoagulation activity (%)
Peptide	0.01 (mM)	0.1 (mM)	1 (mM)	100 (mM)
DLQGK	0.9 ± 0.2	0.9 ± 0.5	2.2 ± 0.3	12.0 ± 0.5

Specific target protein(s)

Specific Target Protein(s):
Prothrombin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with alkaline proteinase Alcalase.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX0623
[D2]	DFBPALAM0014
[D3]	DFBPALGL0005
[D4]	DFBPMUFU0673

[D5]

[D6]

[D7]

[D8]

Reference(s)

Primary literature	Yu Z, Liu B, Zhao W, Yin Y, Liu J, Chen F. Primary and secondary structure of novel ACE-inhibitory peptides from egg white protein. Food Chem. 2012 Jul 15;133(2):315-22. PMID: 25683401 DOI: 10.1016/j.foodchem.2012.01.032
Other literature(s)	N.D
PubDate	2012