DFBP ID - DFBPANTH0094(Antithrombotic peptide)

DFBP ID DFBPANTH0094 Peptide sequence TNGIIR Type Native peptide Peptide/Function name Antithrombotic peptide

Function-activity relationship

Main bioactivity Antithrombotic activity Otheir bioactivity ACE-inhibitory activity [D1], Antioxidative activity [D2], Multifunctional activity [D3]

Calculated physicochemical properties

Three-letter amino acid Thr-Asn-Gly-Ile-Ile-Arg Single-letter amino acid TNGIIR Peptide length 6 Peptide mass Experimental mass Theoretical mass 673.4036 Da 672.77 Da c Net charge 0.00 c Isoelectric point (pI) 11.04 c IC50 N.D pIC50 N.D GRAVY -0.0167 c Hydrophilic residue ratio 50% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Egg white protein Precursor protein Ovalbumin Residue position N.D Precursor protein(s) search Source.1: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity Peptide Anticoagulation activity (%) 0.01 (mM) 0.1 (mM) 1 (mM) 100 (mM) TNGIIR 1.0 ± 0.1 1.1 ± 0.5 2.0 ± 0.5 11.1 ± 0.6 Specific target protein(s) Specific Target Protein(s): Prothrombin

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Non-bitter taste prediction SMILES N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)NCC(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation Enzymatic hydrolysis Enzyme(s)/starter culture Hydrolysis with alkaline proteinase Alcalase.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

DFBP [D1] DFBPACEI0161 [D2] DFBPANOX0630 [D3] DFBPMUFU0064

BIOPEP-UWM [D4] -

APD [D5] -

BioPepDB [D6] -

MBPDB [D7] -

Reference(s)

Primary literature Yu Z, Liu B, Zhao W, Yin Y, Liu J, Chen F. Primary and secondary structure of novel ACE-inhibitory peptides from egg white protein. Food Chem. 2012 Jul 15;133(2):315-22. PMID: 25683401 DOI: 10.1016/j.foodchem.2012.01.032 Other literature(s) N.D PubDate 2012