Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPINPE0001(Other bio-peptides)

DFBP ID	DFBPINPE0001
Peptide sequence	PHPHLSF
Type	Native peptide
Peptide/Function name	Chymosin inhibitor

Function-activity relationship

Main bioactivity	Inhibitor activity
Otheir bioactivity	Antithrombotic activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Pro-His-Pro-His-Leu-Ser-Phe

Single-letter amino acid

PHPHLSF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	833.94 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.95 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5429^c

Hydrophilic residue ratio

57.14%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk
Precursor protein	κ-Casein
Residue position	f(99-105)
Precursor protein(s) search	Source.1: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.2: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.3: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.4: DFBPPR8492 ---- Milk proteins ---- Kappa-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Other bioactive activity	The peptide PHPHLSF showed Chymosin (EC 3.4.23.4) inhibitory acitivity.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANTH0062
[D2]	DFBPMUFU0721

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Xu, R.J. Bioactive peptides in milk and their biological and health implications. Food Reviews International. 1998, 14, 1-16. DOI: 10.1080/87559129809541147
Other literature(s)	N.D
PubDate	1998