Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0010(Opioid peptide)

DFBP ID	DFBPOPIO0010
Peptide sequence	YYGY
Type	Native peptide
Peptide/Function name	Opioid peptide, Lactoferroxin analogues

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Tyr-Gly-Tyr

Single-letter amino acid

YYGY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	564.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.83 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.0750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human whey protein
Precursor protein	Lactoferrin
Residue position	f(537-540)
Precursor protein(s) search	Source.1: DFBPPR1485 ---- Plant proteins ---- Pheophorbide a oxygenase, chloroplastic Source.2: DFBPPR3630 ---- Plant proteins ---- Probable anion transporter 6 Source.3: DFBPPR4149 ---- Plant proteins ---- Probable anion transporter 7 Source.4: DFBPPR5107 ---- Plant proteins ---- Cytochrome c oxidase subunit 2, mitochondrial Source.5: DFBPPR7596 ---- Milk proteins ---- Lactotransferrin Source.6: DFBPPR7658 ---- Milk proteins ---- Lactotransferrin Source.7: DFBPPR7669 ---- Milk proteins ---- Lactotransferrin Source.8: DFBPPR7683 ---- Milk proteins ---- Lactotransferrin Source.9: DFBPPR7713 ---- Milk proteins ---- Lactotransferrin Source.10: DFBPPR8500 ---- Milk proteins ---- Lactotransferrin Source.11: DFBPPR16712 ---- Animal proteins ---- RNA-binding protein 47 Source.12: DFBPPR18952 ---- Animal proteins ---- Serotransferrin Source.13: DFBPPR19309 ---- Animal proteins ---- Cadherin-related family member 1 Source.14: DFBPPR19953 ---- Animal proteins ---- Neurolysin, mitochondrial Source.15: DFBPPR20109 ---- Animal proteins ---- Ovarian cancer G-protein coupled receptor 1 Source.16: DFBPPR20426 ---- Animal proteins ---- Thimet oligopeptidase Source.17: DFBPPR8617 ---- Animal proteins ---- Neurolysin, mitochondrial Source.18: DFBPPR9015 ---- Animal proteins ---- Serotransferrin Source.19: DFBPPR9255 ---- Animal proteins ---- Thimet oligopeptidase Source.20: DFBPPR11405 ---- Animal proteins ---- Cadherin-related family member 1 Source.21: DFBPPR12113 ---- Animal proteins ---- Protein DENND6A Source.22: DFBPPR12251 ---- Animal proteins ---- Serotransferrin Source.23: DFBPPR12388 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1S Source.24: DFBPPR12911 ---- Animal proteins ---- Neurolysin, mitochondrial Source.25: DFBPPR13263 ---- Animal proteins ---- Serotransferrin Source.26: DFBPPR14003 ---- Animal proteins ---- Dihydropyridine-sensitive L-type skeletal muscle calcium channel subunit alpha-1 Source.27: DFBPPR14077 ---- Marine protein ---- Serotransferrin-1 Source.28: DFBPPR14080 ---- Marine protein ---- Serotransferrin-2 Source.29: DFBPPR14903 ---- Microorganism protein ---- Transcription elongation factor SPT6 Source.30: DFBPPR15143 ---- Microorganism protein ---- Low-affinity glucose transporter
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity

The effective bioactive sequence of the peptide is YYGY-OCH₃.
YYGY-OCH₃ have opioid activity as an antagonist peptide and had somewhat higher degrees of preference for κ-receptors than for μ-receptors. As shown in table 1.

Tabel 1 The opioid activity of peptide
Peptides	Receptor affinity (IC₅₀, uM)			Antagonism (pA₂)
	μ	δ	κ	μ, GPI ^a	δ, MVD ^b	κ, RVD ^c
	[³H]DAGO	[³H]DADLE	[³H]EKC	DAGO	DADLE	Dyn. A[1-13]
YYGY-OCH₃	12.0	17.0	131	5.27	4.62	4.60

^a Guinea pig ileum.^b Mouse vas deferens.^c Rabbit vas deferens.

Specific target protein(s)

Specific Target Protein(s):
Kappa-type opioid receptor
Mu-type opioid receptor

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)NCC(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	The peptide was synthesized by conventional solid-phase methods with tert-butyloxy-carbonyl-Tyr (Cl₂Bzl) resin as a starting material.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Tani F, Iio K, Chiba H, Yoshikawa M. Isolation and characterization of opioid antagonist peptides derived from human lactoferrin. Agric Biol Chem. 1990 Jul;54(7):1803-10. PMID: 1369293
Other literature(s)	N.D
PubDate	1990