Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0018(Opioid peptide)

DFBP ID	DFBPOPIO0018
Peptide sequence	GYPMYPLPR
Type	Native peptide
Peptide/Function name	Opioid peptide, Oryzatensin

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	Immunomodulatory activity ^[D1], Ileum contracting activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Gly-Tyr-Pro-Met-Tyr-Pro-Leu-Pro-Arg

Single-letter amino acid

GYPMYPLPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1093.30 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.59 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.7333^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Rice (Oryza sativa L. var. japonica cv.)
Precursor protein	Rice albumin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR3414 ---- Plant proteins ---- Seed allergenic protein RA5
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity

Oryzatensin was synthesized and its activity in longitudinal muscle strips of the guinea pig ileum was examined. DAGO, a μ-opioid agonist peptide, inhibited the electrically-induced twitches in a dose-dependent manner, and oryzatensin completely reversed the inhibition by DAGO at about 0.5 μM. However, these reversed twitches soon decreased. The profile of this anti-opioid activity of ory zatensin differed from that of naloxone, a typical opioid antagonist, which shows a long-lasting effect. Although oryzatensin showed weak affinity for μ-opioid receptors, the apparent anti-opioid activity seemed to be associated with the slower ileumcontraction.

Specific target protein(s)

Specific Target Protein(s):
Mu-type opioid receptor

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The bioactive peptide was isolated from the tryptic digest of rice soluble protein.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPIMMU0006
[D2]	DFBPICPE0001
[D3]	DFBPMUFU0757

BIOPEP-UWM

[D4]

2838, 2839, 2840

APD

[D5]

BioPepDB

[D6]

MBPDB

[D7]

Reference(s)

Primary literature	Takahashi M, Moriguchi S, Yoshikawa M, Sasaki R. Isolation and characterization of oryzatensin: a novel bioactive peptide with ileum-contracting and immunomodulating activities derived from rice albumin. Biochem Mol Biol Int. 1994 Aug;33(6):1151-8. PMID: 7804141
Other literature(s)	N.D
PubDate	1994