E-mail:gzliang@cqu.edu.cn    Tel: (86)2365102507

List of peptide properties
DFBP ID - DFBPPEPI0011(PEP-inhibitory peptide)
DFBP ID DFBPPEPI0011
Peptide sequence VYPFPGPI
Type Native peptide
Peptide/Function name PEP-inhibitory peptide
Function-activity relationship
Main bioactivity PEP-inhibitory activity
Otheir bioactivity ACE-inhibitory activity [D1], Opioid activity [D2], Multifunctional activity [D3]
Calculated physicochemical properties
Three-letter amino acid Val-Tyr-Pro-Phe-Pro-Gly-Pro-Ile
Single-letter amino acid VYPFPGPI
Peptide length 8
Peptide mass
Experimental mass Theoretical mass
N.D 889.05 Da c
Net charge 0.00 c
Isoelectric point (pI) 5.92 c
IC50 N.D
pIC50 N.D
GRAVY 0.6250 c
Hydrophilic residue ratio 87.5% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Animal
Organism/Source Bovine milk
Precursor protein β-Casein
Residue position

f(60-67)

Precursor protein(s) search
Link-research
Link 1: DFBPPEPI0046----Ovine milk----β-Casein
Link 2: DFBPPEPI0047----Buffalo milk----β-Casein
Biological/Functional activity & target protein
PEP-inhibitory activity

Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). The peptide possibly act as anti-amnestic agents. The peptide was assayed in vitro for their capacity to inhibit the Flavobacterium meningosepticum PEP activity, the IC50 value is 110 uM and it's weakly active.

Specific target protein(s) Specific Target Protein(s):
Prolyl endopeptidase
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)O
Preparation method
Mode of preparation

Chemical synthesis

Enzyme(s)/starter culture

Peptide was synthesized by the Fmoc solid-phase method.

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information

The peptide was also found in Ovine, Buffalo (β-casein).

Database cross-references
DFBP
[D1] DFBPACEI0666, DFBPACEI1138, DFBPACEI1139
[D2] DFBPOPIO0112, DFBPOPIO0113, DFBPOPIO0114
[D3] DFBPMUFU0150
BIOPEP-UWM [D4] 2615, 7492
APD [D5] -
BioPepDB [D6] -
MBPDB [D7] -
Reference(s)
Primary literature Asano M, Nio N, Ariyoshi Y. Inhibition of prolyl endopeptidase by synthetic beta-casein peptides and their derivatives with a C-terminal prolinol or prolinal. Biosci Biotechnol Biochem. 1992 Jun;56(6):976-7.
PMID: 1368255
Other literature(s) N.D
PubDate 1992
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214