Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0011(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0011
Peptide sequence	VYPFPGPI
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Opioid activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Val-Tyr-Pro-Phe-Pro-Gly-Pro-Ile

Single-letter amino acid

VYPFPGPI

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	889.05 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.6250^c

Hydrophilic residue ratio

87.5%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk
Precursor protein	β-Casein
Residue position	f(60-67)
Precursor protein(s) search	Source.1: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	Link 1: DFBPPEPI0046----Ovine milk----β-Casein Link 2: DFBPPEPI0047----Buffalo milk----β-Casein

Biological/Functional activity & target protein

PEP-inhibitory activity	Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). The peptide possibly act as anti-amnestic agents. The peptide was assayed in vitro for their capacity to inhibit the Flavobacterium meningosepticum PEP activity, the IC₅₀ value is 110 uM and it's weakly active.
Specific target protein(s)	Specific Target Protein(s): Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)O

Preparation method

Mode of preparation	Chemical synthesis
Enzyme(s)/starter culture	Peptide was synthesized by the Fmoc solid-phase method.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide was also found in Ovine, Buffalo (β-casein).

Database cross-references

DFBP

[D1]	DFBPACEI0666, DFBPACEI1138, DFBPACEI1139
[D2]	DFBPOPIO0112, DFBPOPIO0113, DFBPOPIO0114
[D3]	DFBPMUFU0150

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Asano M, Nio N, Ariyoshi Y. Inhibition of prolyl endopeptidase by synthetic beta-casein peptides and their derivatives with a C-terminal prolinol or prolinal. Biosci Biotechnol Biochem. 1992 Jun;56(6):976-7. PMID: 1368255 DOI: 10.1271/bbb.56.976
Other literature(s)	N.D
PubDate	1992