Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0017(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0017
Peptide sequence	HLPPPV
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

His-Leu-Pro-Pro-Pro-Val

Single-letter amino acid

HLPPPV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	658.79 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.0000^c

Hydrophilic residue ratio

83.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Corn, Maize endosperm meal
Precursor protein	γ-Zein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR5706 ---- Plant proteins ---- Glutelin-2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity

Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). This peptide inhibited PEP from both bacterial and mammalian sources, as shown in the tables:

Table 1. PEP (from F. meningosepticum) inhibitory activity of synthetic peptides.
Peptide	Conc. (uM)	Inhibition (%)	IC₅₀ (uM)
HLPPPV	400	80	80

Table 2. PEP (from Bovine Brain) inhibitory activity of synthetic peptides.
Peptide	Conc. (uM)	Inhibition (%)
HLPPPV	400	28

Specific target protein(s)

Specific Target Protein(s):
Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The γ-zein was hydrolyzed with subtilisin (Carlsberg).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1569
[D2]	DFBPMUFU0434

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Maruyama, S., Miyoshi, S., Osa, T., Tanaka, H. Prolyl endopeptidase inhibitory activity of peptides in the repeated sequence of various proline-rich proteins. Journal of Fermentation and Bioengineering. 1992, 74, 145-8. DOI: 10.1016/0922-338X(92)90073-4
Other literature(s)	[1] Wilson J , Hayes M , Carney B . Angiotensin-I-converting enzyme and prolyl endopeptidase inhibitory peptides from natural sources with a focus on marine processing by-products[J]. Food Chemistry, 2011, 129(2):235-244.
PubDate	1992