Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0020(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0020
Peptide sequence	PPPLP
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Pro-Pro-Pro-Leu-Pro

Single-letter amino acid

PPPLP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	519.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5200^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine brain
Precursor protein	Glial fibrillary acidic protein (GFAP)
Residue position	f(39-43)
Precursor protein(s) search	Source.1: DFBPPR1003 ---- Plant proteins ---- Soluble starch synthase 1, chloroplastic/amyloplastic Source.2: DFBPPR1125 ---- Plant proteins ---- Protein FLOURY ENDOSPERM 6, chloroplastic Source.3: DFBPPR1334 ---- Plant proteins ---- 2-oxoglutarate-dependent dioxygenase 21, chloroplastic Source.4: DFBPPR1399 ---- Plant proteins ---- Phytoene synthase 3, chloroplastic Source.5: DFBPPR1425 ---- Plant proteins ---- Transcription factor BHLH156 Source.6: DFBPPR1653 ---- Plant proteins ---- Protein CHLOROPLAST ENHANCING STRESS TOLERANCE, chloroplastic Source.7: DFBPPR1862 ---- Plant proteins ---- Heat stress transcription factor B-2c Source.8: DFBPPR1916 ---- Plant proteins ---- Protein PYRICULARIA ORYZAE RESISTANCE 21 Source.9: DFBPPR1922 ---- Plant proteins ---- Cyclin-dependent kinase G-2 Source.10: DFBPPR1955 ---- Plant proteins ---- Endoribonuclease Dicer homolog 3b Source.11: DFBPPR2080 ---- Plant proteins ---- Heat stress transcription factor B-1 Source.12: DFBPPR2161 ---- Plant proteins ---- Probable serine/threonine-protein kinase WNK7 Source.13: DFBPPR2274 ---- Plant proteins ---- Wee1-like protein kinase Source.14: DFBPPR2287 ---- Plant proteins ---- Dof zinc finger protein 3 Source.15: DFBPPR2366 ---- Plant proteins ---- Protein NODULATION SIGNALING PATHWAY 2 Source.16: DFBPPR2413 ---- Plant proteins ---- Probable serine/threonine-protein kinase WNK8 Source.17: DFBPPR2568 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 40 Source.18: DFBPPR2736 ---- Plant proteins ---- Ethylene-responsive transcription factor ABI4 Source.19: DFBPPR2817 ---- Plant proteins ---- Early nodulin-like protein 1 Source.20: DFBPPR3125 ---- Plant proteins ---- Putative alpha-L-fucosidase 1 Source.21: DFBPPR3396 ---- Plant proteins ---- Probable transcription factor GLK2 Source.22: DFBPPR3459 ---- Plant proteins ---- Squamosa promoter-binding-like protein 17 Source.23: DFBPPR3600 ---- Plant proteins ---- Transcription factor NIGTH1 Source.24: DFBPPR3757 ---- Plant proteins ---- Probable protein phosphatase 2C 71 Source.25: DFBPPR3801 ---- Plant proteins ---- Transcription termination factor MTERF4, chloroplastic Source.26: DFBPPR3802 ---- Plant proteins ---- Dehydration-responsive element-binding protein 2E Source.27: DFBPPR3977 ---- Plant proteins ---- Formin-like protein 5 Source.28: DFBPPR3986 ---- Plant proteins ---- CRS2-associated factor 2, chloroplastic Source.29: DFBPPR4007 ---- Plant proteins ---- Zinc-finger homeodomain protein 7 Source.30: DFBPPR4172 ---- Plant proteins ---- Dof zinc finger protein 4 Source.31: DFBPPR4186 ---- Plant proteins ---- Formin-like protein 18 Source.32: DFBPPR4212 ---- Plant proteins ---- RNA pseudouridine synthase 1 Source.33: DFBPPR4232 ---- Plant proteins ---- Formin-like protein 14 Source.34: DFBPPR4283 ---- Plant proteins ---- SCAR-like protein 1 Source.35: DFBPPR4315 ---- Plant proteins ---- Organelle RRM domain-containing protein 1, chloroplastic Source.36: DFBPPR4386 ---- Plant proteins ---- WUSCHEL-related homeobox 5 Source.37: DFBPPR4394 ---- Plant proteins ---- Formin-like protein 9 Source.38: DFBPPR4419 ---- Plant proteins ---- Formin-like protein 15 Source.39: DFBPPR4423 ---- Plant proteins ---- SCAR-like protein 2 Source.40: DFBPPR4444 ---- Plant proteins ---- Formin-like protein 6 Source.41: DFBPPR4477 ---- Plant proteins ---- Formin-like protein 3 Source.42: DFBPPR4480 ---- Plant proteins ---- Formin-like protein 7 Source.43: DFBPPR4481 ---- Plant proteins ---- Formin-like protein 12 Source.44: DFBPPR4486 ---- Plant proteins ---- CASP-like protein 4B1 Source.45: DFBPPR4534 ---- Plant proteins ---- Protein argonaute 4A Source.46: DFBPPR4706 ---- Plant proteins ---- Tubby-like protein 4 Source.47: DFBPPR4747 ---- Plant proteins ---- Protein Brevis radix-like 2 Source.48: DFBPPR4775 ---- Plant proteins ---- B3 domain-containing protein Os03g0120900 Source.49: DFBPPR4807 ---- Plant proteins ---- Protein MEI2-like 6 Source.50: DFBPPR4902 ---- Plant proteins ---- Endoribonuclease Dicer homolog 1 Source.51: DFBPPR4903 ---- Plant proteins ---- E3 ubiquitin-protein ligase EL5 Source.52: DFBPPR4982 ---- Plant proteins ---- Probable aspartic proteinase GIP1 Source.53: DFBPPR5565 ---- Plant proteins ---- Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic Source.54: DFBPPR5614 ---- Plant proteins ---- Protein SCARECROW Source.55: DFBPPR5781 ---- Plant proteins ---- CLAVATA3/ESR (CLE)-related protein ESR3 Source.56: DFBPPR6665 ---- Plant proteins ---- DELLA protein RHT-1 Source.57: DFBPPR7088 ---- Plant proteins ---- DELLA protein SLN1 Source.58: DFBPPR7192 ---- Plant proteins ---- 4-hydroxyphenylpyruvate dioxygenase Source.59: DFBPPR15982 ---- Animal proteins ---- Triadin Source.60: DFBPPR15983 ---- Animal proteins ---- Tight junction protein ZO-1 Source.61: DFBPPR16261 ---- Animal proteins ---- Retinoic acid receptor alpha Source.62: DFBPPR16668 ---- Animal proteins ---- Zinc finger and BTB domain-containing protein 22 Source.63: DFBPPR16828 ---- Animal proteins ---- Coagulation factor X Source.64: DFBPPR17473 ---- Animal proteins ---- E3 ubiquitin-protein ligase TRIM71 Source.65: DFBPPR17525 ---- Animal proteins ---- Neural Wiskott-Aldrich syndrome protein Source.66: DFBPPR17900 ---- Animal proteins ---- Acetyl-CoA carboxylase 1 Source.67: DFBPPR17961 ---- Animal proteins ---- Cyclin-dependent kinase 12 Source.68: DFBPPR18063 ---- Animal proteins ---- Microtubule-associated protein 1S Source.69: DFBPPR18134 ---- Animal proteins ---- Cyclin-T1 Source.70: DFBPPR18411 ---- Animal proteins ---- Inhibin beta B chain Source.71: DFBPPR18571 ---- Animal proteins ---- CREB-regulated transcription coactivator 2 Source.72: DFBPPR18729 ---- Animal proteins ---- Creatine kinase U-type, mitochondrial Source.73: DFBPPR18947 ---- Animal proteins ---- Thyroid receptor-interacting protein 6 Source.74: DFBPPR19306 ---- Animal proteins ---- Splicing factor 3A subunit 1 Source.75: DFBPPR19339 ---- Animal proteins ---- Peroxiredoxin-4 Source.76: DFBPPR19588 ---- Animal proteins ---- Prostaglandin reductase 2 Source.77: DFBPPR19635 ---- Animal proteins ---- Glial fibrillary acidic protein Source.78: DFBPPR19640 ---- Animal proteins ---- Prolargin Source.79: DFBPPR20169 ---- Animal proteins ---- Cytoplasmic dynein 2 light intermediate chain 1 Source.80: DFBPPR20505 ---- Animal proteins ---- T-box transcription factor TBX6 Source.81: DFBPPR20999 ---- Animal proteins ---- Protein SERAC1 Source.82: DFBPPR21189 ---- Animal proteins ---- Dynein assembly factor 1, axonemal Source.83: DFBPPR21294 ---- Animal proteins ---- Actin filament-associated protein 1-like 1 Source.84: DFBPPR21350 ---- Animal proteins ---- Nuclear apoptosis-inducing factor 1 Source.85: DFBPPR21823 ---- Animal proteins ---- Zinc finger protein 526 Source.86: DFBPPR21896 ---- Animal proteins ---- Protein CNPPD1 Source.87: DFBPPR22294 ---- Animal proteins ---- Ubinuclein-2 Source.88: DFBPPR22434 ---- Animal proteins ---- UPF0692 protein C19orf54 homolog Source.89: DFBPPR22442 ---- Animal proteins ---- ZAR1-like protein Source.90: DFBPPR22651 ---- Animal proteins ---- Spermatogenesis-associated protein 2-like protein Source.91: DFBPPR8534 ---- Animal proteins ---- Zona pellucida sperm-binding protein 4 Source.92: DFBPPR8679 ---- Animal proteins ---- Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 Source.93: DFBPPR8767 ---- Animal proteins ---- Lutropin-choriogonadotropic hormone receptor Source.94: DFBPPR9055 ---- Animal proteins ---- Ameloblastin Source.95: DFBPPR9085 ---- Animal proteins ---- Membrane-associated progesterone receptor component 1 Source.96: DFBPPR9372 ---- Animal proteins ---- Creatine kinase U-type, mitochondrial Source.97: DFBPPR10223 ---- Animal proteins ---- Retinoic acid receptor alpha Source.98: DFBPPR10247 ---- Animal proteins ---- Actin filament-associated protein 1 Source.99: DFBPPR10270 ---- Animal proteins ---- Agrin Source.100: DFBPPR10351 ---- Animal proteins ---- Protein sidekick-2 Source.101: DFBPPR10554 ---- Animal proteins ---- Creatine kinase S-type, mitochondrial Source.102: DFBPPR10565 ---- Animal proteins ---- Acetylcholine receptor subunit delta Source.103: DFBPPR10638 ---- Animal proteins ---- Cellular tumor antigen p53 Source.104: DFBPPR10665 ---- Animal proteins ---- Mitochondrial fission regulator 1 Source.105: DFBPPR10939 ---- Animal proteins ---- Membrane-associated progesterone receptor component 1 Source.106: DFBPPR10940 ---- Animal proteins ---- Protein piccolo Source.107: DFBPPR11037 ---- Animal proteins ---- Disheveled-associated activator of morphogenesis 2 Source.108: DFBPPR11096 ---- Animal proteins ---- WASH complex subunit 1 Source.109: DFBPPR11146 ---- Animal proteins ---- Formin Source.110: DFBPPR11251 ---- Animal proteins ---- Transcriptional enhancer factor TEF-5 Source.111: DFBPPR11413 ---- Animal proteins ---- Tudor domain-containing protein 7 Source.112: DFBPPR11595 ---- Animal proteins ---- Histone-lysine N-methyltransferase SETD1B Source.113: DFBPPR11788 ---- Animal proteins ---- Homeobox protein GBX-2 Source.114: DFBPPR11893 ---- Animal proteins ---- Laminin subunit beta-1 variant Source.115: DFBPPR11901 ---- Animal proteins ---- Zinc finger CCHC domain-containing protein 8 Source.116: DFBPPR11906 ---- Animal proteins ---- Ubiquitin-associated domain-containing protein 1 Source.117: DFBPPR12262 ---- Animal proteins ---- Zona pellucida sperm-binding protein 4 Source.118: DFBPPR12651 ---- Animal proteins ---- Cytochrome P450 2B5 Source.119: DFBPPR12718 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform Source.120: DFBPPR12758 ---- Animal proteins ---- Creatine kinase S-type, mitochondrial Source.121: DFBPPR13298 ---- Animal proteins ---- Cyclin-T1 Source.122: DFBPPR13334 ---- Animal proteins ---- Anion exchange protein 2 Source.123: DFBPPR13603 ---- Animal proteins ---- Acetyl-CoA carboxylase 1 Source.124: DFBPPR14262 ---- Marine protein ---- Pro-opiomelanocortin Source.125: DFBPPR14991 ---- Microorganism protein ---- Actin cytoskeleton-regulatory complex protein PAN1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity	Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). This peptide showed the K_i value of 674 uM.
Specific target protein(s)	Specific Target Protein(s): Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Chmical synthesis
Enzyme(s)/starter culture	The peptide was synthesized by the solid phase method with a 430A Peptide Synthesizer (Applied Biosystems. Inc.).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Maruyama S, Ohmori T, Nakagami T. Prolylendopeptidase inhibitory activity of a glial fibrillary acidic protein fragment and other proline-rich peptides. Biosci Biotechnol Biochem. 1996 Feb;60(2):358-9. PMID: 9063991 DOI: 10.1271/bbb.60.358
Other literature(s)	N.D
PubDate	1996