Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0021(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0021
Peptide sequence	MPPPLP
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	DPP IV-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Met-Pro-Pro-Pro-Leu-Pro

Single-letter amino acid

MPPPLP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	650.82 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.1167^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine brain
Precursor protein	Glial fibrillary acidic protein (GFAP)
Residue position	f(38-43)
Precursor protein(s) search	Source.1: DFBPPR3396 ---- Plant proteins ---- Probable transcription factor GLK2 Source.2: DFBPPR4982 ---- Plant proteins ---- Probable aspartic proteinase GIP1 Source.3: DFBPPR19306 ---- Animal proteins ---- Splicing factor 3A subunit 1 Source.4: DFBPPR19635 ---- Animal proteins ---- Glial fibrillary acidic protein Source.5: DFBPPR11595 ---- Animal proteins ---- Histone-lysine N-methyltransferase SETD1B
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity	Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). MPPPLP inhibited PEP most potently with a K_i (8.8 uM) similar to that of GFAP-(38-55) (8.6 uM).
Specific target protein(s)	Specific Target Protein(s): Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCSC)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Chmical synthesis
Enzyme(s)/starter culture	The peptide was synthesized by the solid phase method with a 430A Peptide Synthesizer (Applied Biosystems. Inc.).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

GFAP-(38-55) and the cleaved short peptide MPPPLP-(38-43) may be acting as PEP inhibitors in the brain.

Database cross-references

DFBP

[D1]	DFBPDPIV0125
[D2]	DFBPMUFU0731

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Maruyama S, Ohmori T, Nakagami T. Prolylendopeptidase inhibitory activity of a glial fibrillary acidic protein fragment and other proline-rich peptides. Biosci Biotechnol Biochem. 1996 Feb;60(2):358-9. PMID: 9063991 DOI: 10.1271/bbb.60.358
Other literature(s)	N.D
PubDate	1996