Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0022(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0022
Peptide sequence	VHLP
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-His-Leu-Pro

Single-letter amino acid

VHLP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
465 Da	464.56 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.8000^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Corn, Maize endosperm meal
Precursor protein	γ-Zein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0929 ---- Plant proteins ---- Protein ROS1A Source.2: DFBPPR0934 ---- Plant proteins ---- LRR receptor-like serine/threonine-protein kinase FLS2 Source.3: DFBPPR1018 ---- Plant proteins ---- Disease resistance protein RGA5 Source.4: DFBPPR1268 ---- Plant proteins ---- Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic Source.5: DFBPPR1599 ---- Plant proteins ---- Adenylosuccinate synthetase 2, chloroplastic Source.6: DFBPPR1764 ---- Plant proteins ---- B3 domain-containing protein VP1 Source.7: DFBPPR2043 ---- Plant proteins ---- Cyclin-dependent kinase E-1 Source.8: DFBPPR2170 ---- Plant proteins ---- Signal peptide peptidase-like 3 Source.9: DFBPPR2432 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.10: DFBPPR2453 ---- Plant proteins ---- Dihydroorotase, mitochondrial Source.11: DFBPPR2925 ---- Plant proteins ---- Two-component response regulator ORR13 Source.12: DFBPPR3459 ---- Plant proteins ---- Squamosa promoter-binding-like protein 17 Source.13: DFBPPR4801 ---- Plant proteins ---- Putative B3 domain-containing protein Os03g0619850 Source.14: DFBPPR4857 ---- Plant proteins ---- B3 domain-containing protein Os03g0619600 Source.15: DFBPPR5344 ---- Plant proteins ---- Nodulin-16 Source.16: DFBPPR5615 ---- Plant proteins ---- Regulatory protein viviparous-1 Source.17: DFBPPR5706 ---- Plant proteins ---- Glutelin-2 Source.18: DFBPPR5816 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.19: DFBPPR6807 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.20: DFBPPR7177 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.21: DFBPPR7616 ---- Milk proteins ---- Macrophage mannose receptor 1 Source.22: DFBPPR16027 ---- Animal proteins ---- Rho GTPase-activating protein 35 Source.23: DFBPPR16193 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 1 Source.24: DFBPPR16211 ---- Animal proteins ---- Lysine-specific demethylase 5C Source.25: DFBPPR16232 ---- Animal proteins ---- Lysine-specific demethylase 5D Source.26: DFBPPR16382 ---- Animal proteins ---- Platelet glycoprotein Ib alpha chain Source.27: DFBPPR16553 ---- Animal proteins ---- Tapasin Source.28: DFBPPR17737 ---- Animal proteins ---- Inactive serine/threonine-protein kinase TEX14 Source.29: DFBPPR18002 ---- Animal proteins ---- Toll-like receptor 10 Source.30: DFBPPR18335 ---- Animal proteins ---- Septin-11 Source.31: DFBPPR18876 ---- Animal proteins ---- Adenylosuccinate synthetase isozyme 1 Source.32: DFBPPR19254 ---- Animal proteins ---- Periaxin Source.33: DFBPPR19612 ---- Animal proteins ---- Lysosomal-trafficking regulator Source.34: DFBPPR19643 ---- Animal proteins ---- Chromodomain-helicase-DNA-binding protein 1-like Source.35: DFBPPR19747 ---- Animal proteins ---- Organic solute transporter subunit beta Source.36: DFBPPR19949 ---- Animal proteins ---- Syndecan-2 Source.37: DFBPPR20068 ---- Animal proteins ---- H2.0-like homeobox protein Source.38: DFBPPR20273 ---- Animal proteins ---- Uridine diphosphate glucose pyrophosphatase NUDT14 Source.39: DFBPPR20623 ---- Animal proteins ---- Retina and anterior neural fold homeobox protein 2 Source.40: DFBPPR21008 ---- Animal proteins ---- Gamma-glutamylaminecyclotransferase Source.41: DFBPPR21659 ---- Animal proteins ---- Peptide chain release factor 1-like, mitochondrial Source.42: DFBPPR22572 ---- Animal proteins ---- Maestro heat-like repeat-containing protein family member 1 Source.43: DFBPPR22586 ---- Animal proteins ---- Uncharacterized protein KIAA2012 homolog Source.44: DFBPPR22703 ---- Animal proteins ---- Arrestin domain-containing protein 5 Source.45: DFBPPR8992 ---- Animal proteins ---- Hyaluronidase-3 Source.46: DFBPPR9100 ---- Animal proteins ---- Ras-related protein Rab-32 Source.47: DFBPPR9105 ---- Animal proteins ---- Lysine-specific demethylase 5C Source.48: DFBPPR9201 ---- Animal proteins ---- Adenylosuccinate synthetase isozyme 1 Source.49: DFBPPR10119 ---- Animal proteins ---- Down syndrome cell adhesion molecule homolog Source.50: DFBPPR10408 ---- Animal proteins ---- Beta-galactoside-binding lectin Source.51: DFBPPR10986 ---- Animal proteins ---- Phosphoglycerate kinase Source.52: DFBPPR11724 ---- Animal proteins ---- Protein TENP Source.53: DFBPPR12009 ---- Animal proteins ---- Retrovirus-related Pol polyprotein Source.54: DFBPPR12146 ---- Animal proteins ---- Transmembrane protein adipocyte-associated 1 homolog Source.55: DFBPPR12279 ---- Animal proteins ---- Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform Source.56: DFBPPR13199 ---- Animal proteins ---- Hemoglobin subunit alpha Source.57: DFBPPR13289 ---- Animal proteins ---- Leukocyte elastase inhibitor Source.58: DFBPPR13295 ---- Animal proteins ---- Alpha-1-antiproteinase 2 Source.59: DFBPPR14994 ---- Microorganism protein ---- Serine/threonine-protein kinase MEC1 Source.60: DFBPPR15017 ---- Microorganism protein ---- Serine/threonine-protein kinase TEL1 Source.61: DFBPPR15868 ---- Microorganism protein ---- Thymidylate synthase Source.62: DFBPPR7767 ---- Plant protein ---- Adenylosuccinate synthetase 2, chloroplastic Source.63: DFBPPR7809 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta'
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity

This peptide had no inhibitory activity against Prolyl endopeptidase (EC:3.4.21.26) from bacterial sources, as shown in the table1:

Table 1. PEP (from F. meningosepticum) inhibitory activity of synthetic peptides.
Peptide	Conc. (uM)	Inhibition (%)	IC₅₀ (uM)
VHLP	800	0	N.D

Specific target protein(s)

Specific Target Protein(s):
Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The γ-zein was hydrolyzed with subtilisin (Carlsberg).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Although this peptide has no PEP-inhibitory activity, its activity value was determined in the literature, so it was included in the database.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Maruyama, S., Miyoshi, S., Osa, T., Tanaka, H. Prolyl endopeptidase inhibitory activity of peptides in the repeated sequence of various proline-rich proteins. Journal of Fermentation and Bioengineering. 1992, 74, 145-8. DOI: 10.1016/0922-338X(92)90073-4
Other literature(s)	N.D
PubDate	1992