Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0025(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0025
Peptide sequence	KPPI
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Pro-Pro-Ile

Single-letter amino acid

KPPI

Peptide length

Peptide mass

Experimental mass	Theoretical mass
454 Da	453.57 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6500^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soybean
Precursor protein	Soybean cell wall protein (SbPRP1)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0819 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC1 Source.2: DFBPPR0938 ---- Plant proteins ---- Mitogen-activated protein kinase 1 Source.3: DFBPPR1089 ---- Plant proteins ---- Protein TOPLESS-RELATED PROTEIN 2 Source.4: DFBPPR2358 ---- Plant proteins ---- Germin-like protein 8-11 Source.5: DFBPPR2361 ---- Plant proteins ---- Iron-phytosiderophore transporter YSL15 Source.6: DFBPPR2362 ---- Plant proteins ---- Metal-nicotianamine transporter YSL2 Source.7: DFBPPR2369 ---- Plant proteins ---- Kinesin-like protein KIN-UB Source.8: DFBPPR2406 ---- Plant proteins ---- Probable serine/threonine-protein kinase WNK6 Source.9: DFBPPR2469 ---- Plant proteins ---- Germin-like protein 8-4 Source.10: DFBPPR2480 ---- Plant proteins ---- Germin-like protein 8-7 Source.11: DFBPPR2501 ---- Plant proteins ---- Germin-like protein 8-10 Source.12: DFBPPR2505 ---- Plant proteins ---- Germin-like protein 8-5 Source.13: DFBPPR2823 ---- Plant proteins ---- Germin-like protein 8-9 Source.14: DFBPPR2827 ---- Plant proteins ---- Germin-like protein 8-8 Source.15: DFBPPR2850 ---- Plant proteins ---- Germin-like protein 8-6 Source.16: DFBPPR2960 ---- Plant proteins ---- 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 Source.17: DFBPPR3067 ---- Plant proteins ---- 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 Source.18: DFBPPR3084 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL13 Source.19: DFBPPR3334 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL12 Source.20: DFBPPR3406 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL16 Source.21: DFBPPR3683 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL14 Source.22: DFBPPR3903 ---- Plant proteins ---- 60S ribosomal protein L2, mitochondrial Source.23: DFBPPR3977 ---- Plant proteins ---- Formin-like protein 5 Source.24: DFBPPR4239 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL10 Source.25: DFBPPR4327 ---- Plant proteins ---- Lysine--tRNA ligase Source.26: DFBPPR4784 ---- Plant proteins ---- Protein DEHYDRATION-INDUCED 19 Source.27: DFBPPR5164 ---- Plant proteins ---- Repetitive proline-rich cell wall protein 2 Source.28: DFBPPR5182 ---- Plant proteins ---- Repetitive proline-rich cell wall protein 1 Source.29: DFBPPR5274 ---- Plant proteins ---- Early nodulin-75 Source.30: DFBPPR5660 ---- Plant proteins ---- Iron-phytosiderophore transporter yellow stripe 1 Source.31: DFBPPR15965 ---- Animal proteins ---- Dystroglycan Source.32: DFBPPR16573 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 2 Source.33: DFBPPR17338 ---- Animal proteins ---- Unconventional myosin-VI Source.34: DFBPPR17354 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.35: DFBPPR17379 ---- Animal proteins ---- Dematin Source.36: DFBPPR17634 ---- Animal proteins ---- Hypoxia-inducible factor 1-alpha Source.37: DFBPPR18040 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase F Source.38: DFBPPR18138 ---- Animal proteins ---- AP-1 complex subunit mu-1 Source.39: DFBPPR18156 ---- Animal proteins ---- Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 Source.40: DFBPPR19154 ---- Animal proteins ---- Cytoplasmic dynein 1 intermediate chain 2 Source.41: DFBPPR19421 ---- Animal proteins ---- Zinc finger-containing ubiquitin peptidase 1 Source.42: DFBPPR19489 ---- Animal proteins ---- Keratocan Source.43: DFBPPR19591 ---- Animal proteins ---- Phosphorylated adapter RNA export protein Source.44: DFBPPR19640 ---- Animal proteins ---- Prolargin Source.45: DFBPPR21379 ---- Animal proteins ---- Phosphoribosyl pyrophosphate synthase-associated protein 2 Source.46: DFBPPR21404 ---- Animal proteins ---- Phosphoribosyl pyrophosphate synthase-associated protein 1 Source.47: DFBPPR21953 ---- Animal proteins ---- Clusterin-like protein 1 Source.48: DFBPPR22295 ---- Animal proteins ---- Protein PROCA1 Source.49: DFBPPR8682 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.50: DFBPPR8705 ---- Animal proteins ---- Unconventional myosin-VI Source.51: DFBPPR8742 ---- Animal proteins ---- Low-density lipoprotein receptor-related protein 2 Source.52: DFBPPR9977 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.53: DFBPPR10475 ---- Animal proteins ---- Adenylate cyclase type 9 Source.54: DFBPPR10940 ---- Animal proteins ---- Protein piccolo Source.55: DFBPPR11300 ---- Animal proteins ---- MAM domain-containing glycosylphosphatidylinositol anchor protein 1 Source.56: DFBPPR12005 ---- Animal proteins ---- Phosphoribosyl pyrophosphate synthase-associated protein 2 Source.57: DFBPPR14886 ---- Microorganism protein ---- Serine/threonine-protein kinase SSN3 Source.58: DFBPPR15208 ---- Microorganism protein ---- Lon protease homolog 2, peroxisomal
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity

Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). This peptide inhibited PEP from bacterial sources to some degree, as shown in the table1:

Table 1. PEP (from F. meningosepticum) inhibitory activity of synthetic peptides.
Peptide	Conc. (uM)	Inhibition (%)	IC₅₀ (uM)
KPPI	400	52	380

Specific target protein(s)

Specific Target Protein(s):
Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)O

Preparation method

Mode of preparation	Chemical synthesis
Enzyme(s)/starter culture	The peptide was synthesized by the solid phase method with a 430A Peptide Synthesizer (Applied Biosystems, Inc.).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Maruyama, S., Miyoshi, S., Osa, T., Tanaka, H. Prolyl endopeptidase inhibitory activity of peptides in the repeated sequence of various proline-rich proteins. Journal of Fermentation and Bioengineering. 1992, 74, 145-8. DOI: 10.1016/0922-338X(92)90073-4
Other literature(s)	N.D
PubDate	1992