Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0032(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0032
Peptide sequence	TPPV
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Thr-Pro-Pro-Val

Single-letter amino acid

TPPV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
413 Da	412.48 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.0750^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Carrot
Precursor protein	Carrot 33-kDa protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0830 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC6 Source.2: DFBPPR1095 ---- Plant proteins ---- Transcription factor GAMYB Source.3: DFBPPR1100 ---- Plant proteins ---- Soluble starch synthase 2-3, chloroplastic/amyloplastic Source.4: DFBPPR1548 ---- Plant proteins ---- Ent-copalyl diphosphate synthase 1, chloroplastic Source.5: DFBPPR1588 ---- Plant proteins ---- Replication protein A 32 kDa subunit C Source.6: DFBPPR1675 ---- Plant proteins ---- Protein LSD1 Source.7: DFBPPR1712 ---- Plant proteins ---- Mixed-linked glucan synthase 4 Source.8: DFBPPR1861 ---- Plant proteins ---- Laccase-25 Source.9: DFBPPR2155 ---- Plant proteins ---- Two-component response regulator-like PRR1 Source.10: DFBPPR2688 ---- Plant proteins ---- Regulatory-associated protein of TOR 2 Source.11: DFBPPR2735 ---- Plant proteins ---- Expansin-A24 Source.12: DFBPPR2757 ---- Plant proteins ---- Probable glucuronosyltransferase Os01g0157700 Source.13: DFBPPR2765 ---- Plant proteins ---- Regulatory-associated protein of TOR 1 Source.14: DFBPPR3319 ---- Plant proteins ---- Transcription factor TGAL8 Source.15: DFBPPR3792 ---- Plant proteins ---- Phosphopantetheine adenylyltransferase 1 Source.16: DFBPPR4233 ---- Plant proteins ---- Putative glutaredoxin-C2 Source.17: DFBPPR4399 ---- Plant proteins ---- IAA-amino acid hydrolase ILR1-like 5 Source.18: DFBPPR4866 ---- Plant proteins ---- Putative B3 domain-containing protein Os02g0455900 Source.19: DFBPPR5182 ---- Plant proteins ---- Repetitive proline-rich cell wall protein 1 Source.20: DFBPPR5415 ---- Plant proteins ---- Eudesmanediol synthase Source.21: DFBPPR5634 ---- Plant proteins ---- Eudesmanediol synthase Source.22: DFBPPR5665 ---- Plant proteins ---- Extensin Source.23: DFBPPR5973 ---- Plant proteins ---- Cortical cell-delineating protein Source.24: DFBPPR5977 ---- Plant proteins ---- Putative AC transposase Source.25: DFBPPR6263 ---- Plant proteins ---- Chlorophyll a-b binding protein 3, chloroplastic Source.26: DFBPPR6282 ---- Plant proteins ---- Rhicadhesin receptor Source.27: DFBPPR6515 ---- Plant proteins ---- Carotenoid 9,10(9',10')-cleavage dioxygenase 1 Source.28: DFBPPR7649 ---- Milk proteins ---- Cadherin-1 Source.29: DFBPPR15993 ---- Animal proteins ---- Sodium/myo-inositol cotransporter Source.30: DFBPPR16024 ---- Animal proteins ---- Podocalyxin Source.31: DFBPPR16064 ---- Animal proteins ---- Calnexin Source.32: DFBPPR17270 ---- Animal proteins ---- Nucleophosmin Source.33: DFBPPR17910 ---- Animal proteins ---- Mitogen-activated protein kinase kinase kinase 13 Source.34: DFBPPR18003 ---- Animal proteins ---- 7-dehydrocholesterol reductase Source.35: DFBPPR18052 ---- Animal proteins ---- Calcium-activated chloride channel regulator 1 Source.36: DFBPPR18082 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A activator Source.37: DFBPPR18122 ---- Animal proteins ---- Microtubule-associated protein 4 Source.38: DFBPPR18134 ---- Animal proteins ---- Cyclin-T1 Source.39: DFBPPR18231 ---- Animal proteins ---- Transcription factor jun-B Source.40: DFBPPR18438 ---- Animal proteins ---- Angiopoietin-related protein 4 Source.41: DFBPPR18784 ---- Animal proteins ---- Thrombospondin-4 Source.42: DFBPPR19578 ---- Animal proteins ---- Dimethyladenosine transferase 2, mitochondrial Source.43: DFBPPR19628 ---- Animal proteins ---- Mothers against decapentaplegic homolog 2 Source.44: DFBPPR19697 ---- Animal proteins ---- Sodium/myo-inositol cotransporter Source.45: DFBPPR20615 ---- Animal proteins ---- Seizure 6-like protein 2 Source.46: DFBPPR20998 ---- Animal proteins ---- Zinc finger protein 821 Source.47: DFBPPR21017 ---- Animal proteins ---- Mediator of RNA polymerase II transcription subunit 26 Source.48: DFBPPR21024 ---- Animal proteins ---- Glycosylated lysosomal membrane protein Source.49: DFBPPR21656 ---- Animal proteins ---- Thioredoxin domain-containing protein 11 Source.50: DFBPPR21885 ---- Animal proteins ---- DNA polymerase epsilon subunit 2 Source.51: DFBPPR21946 ---- Animal proteins ---- Zinc finger protein 414 Source.52: DFBPPR8576 ---- Animal proteins ---- Hormone-sensitive lipase Source.53: DFBPPR8649 ---- Animal proteins ---- Acrosin Source.54: DFBPPR10429 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-5 Source.55: DFBPPR10505 ---- Animal proteins ---- DNA-binding protein Ikaros Source.56: DFBPPR10559 ---- Animal proteins ---- Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 Source.57: DFBPPR10729 ---- Animal proteins ---- Versican core protein Source.58: DFBPPR10909 ---- Animal proteins ---- Transcription factor 12 Source.59: DFBPPR11135 ---- Animal proteins ---- Popeye domain-containing protein 3 Source.60: DFBPPR11136 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase gamma Source.61: DFBPPR11341 ---- Animal proteins ---- CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, mitochondrial Source.62: DFBPPR11794 ---- Animal proteins ---- Nuclear protein MDM1 Source.63: DFBPPR11885 ---- Animal proteins ---- ELL-associated factor 2 Source.64: DFBPPR12336 ---- Animal proteins ---- Apolipoprotein D Source.65: DFBPPR12412 ---- Animal proteins ---- Potassium voltage-gated channel subfamily D member 2 Source.66: DFBPPR12479 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A activator Source.67: DFBPPR12557 ---- Animal proteins ---- Acrosin Source.68: DFBPPR13298 ---- Animal proteins ---- Cyclin-T1 Source.69: DFBPPR13339 ---- Animal proteins ---- Runt-related transcription factor 2 Source.70: DFBPPR13602 ---- Animal proteins ---- Acrosin Source.71: DFBPPR14015 ---- Animal proteins ---- Cytochrome c oxidase subunit 3 Source.72: DFBPPR14119 ---- Marine protein ---- Cytochrome c oxidase subunit 3 Source.73: DFBPPR14377 ---- Marine protein ---- DNA-directed RNA polymerase subunit beta Source.74: DFBPPR14627 ---- Marine protein ---- Cytochrome c oxidase subunit 3 Source.75: DFBPPR14887 ---- Microorganism protein ---- Histone acetyltransferase ESA1 Source.76: DFBPPR15031 ---- Microorganism protein ---- NAD-dependent histone deacetylase SIR2 Source.77: DFBPPR15115 ---- Microorganism protein ---- Helicase SWR1 Source.78: DFBPPR15351 ---- Microorganism protein ---- Protein transport protein SEC31 Source.79: DFBPPR15487 ---- Microorganism protein ---- Restriction of telomere capping protein 1 Source.80: DFBPPR15503 ---- Microorganism protein ---- Glycosylphosphatidylinositol anchor biosynthesis protein 11 Source.81: DFBPPR15577 ---- Microorganism protein ---- Chromatin modification-related protein EAF7 Source.82: DFBPPR15678 ---- Microorganism protein ---- Autophagy-related protein 2 Source.83: DFBPPR7832 ---- Plant protein ---- Extensin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity

Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). This peptide inhibited PEP from bacterial sources to some degree, as shown in the table1:

Table 1. PEP (from F. meningosepticum) inhibitory activity of synthetic peptides.
Peptide	Conc. (uM)	Inhibition (%)	IC₅₀ (uM)
TPPV	400	12	N.D

Specific target protein(s)

Specific Target Protein(s):
Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Chemical synthesis
Enzyme(s)/starter culture	The peptide was synthesized by the solid phase method with a 430A Peptide Synthesizer (Applied Biosystems, Inc.).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Maruyama, S., Miyoshi, S., Osa, T., Tanaka, H. Prolyl endopeptidase inhibitory activity of peptides in the repeated sequence of various proline-rich proteins. Journal of Fermentation and Bioengineering. 1992, 74, 145-8. DOI: 10.1016/0922-338X(92)90073-4
Other literature(s)	N.D
PubDate	1992