Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPRENI0007(Renin-inhibitory peptide)

DFBP ID	DFBPRENI0007
Peptide sequence	MDSQ
Type	Native peptide
Peptide/Function name	Renin-inhibitory peptide

Function-activity relationship

Main bioactivity	Renin-inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Met-Asp-Ser-Gln

Single-letter amino acid

MDSQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
479 Da	479.50 Da ^c

Net charge

-1

Isoelectric point (pI)

3.10

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Rapeseed
Precursor protein	Cruciferin
Residue position	f(117-121)
Precursor protein(s) search	Source.1: DFBPPR7445 ---- Plant proteins ---- Cruciferin CRU1 Source.2: DFBPPR16676 ---- Animal proteins ---- Olfactory receptor-like protein OLF2 Source.3: DFBPPR16785 ---- Animal proteins ---- Metallothionein-1 Source.4: DFBPPR17339 ---- Animal proteins ---- Pre-mRNA-processing factor 19 Source.5: DFBPPR18001 ---- Animal proteins ---- Syntaxin-1B Source.6: DFBPPR18267 ---- Animal proteins ---- Actin-related protein 2 Source.7: DFBPPR8559 ---- Animal proteins ---- Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial Source.8: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.9: DFBPPR8610 ---- Animal proteins ---- Signal transducer and activator of transcription 5B Source.10: DFBPPR9971 ---- Animal proteins ---- Ovotransferrin Source.11: DFBPPR10123 ---- Animal proteins ---- Ephrin type-A receptor 3 Source.12: DFBPPR10414 ---- Animal proteins ---- Pre-mRNA-processing factor 19 Source.13: DFBPPR10556 ---- Animal proteins ---- Tenascin-R Source.14: DFBPPR11196 ---- Animal proteins ---- Mitochondrial Rho GTPase 2 Source.15: DFBPPR12039 ---- Animal proteins ---- Protein GOLM2 Source.16: DFBPPR12227 ---- Animal proteins ---- Cerebellar degeneration-related protein 2 Source.17: DFBPPR12281 ---- Animal proteins ---- Ryanodine receptor 1 Source.18: DFBPPR12842 ---- Animal proteins ---- Multidrug and toxin extrusion protein 2 Source.19: DFBPPR13779 ---- Animal proteins ---- Syntaxin-1B Source.20: DFBPPR14112 ---- Marine protein ---- Proteasome subunit beta type-6-A like protein Source.21: DFBPPR14114 ---- Marine protein ---- Proteasome subunit beta type-6-B like protein Source.22: DFBPPR14652 ---- Marine protein ---- Hypoxia-inducible factor 1-alpha
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Renin-inhibitory activity	The final rapeseed protein hydrolysate (FRPH) exhibited potent Renin (EC:3.4.23.15) inhibitory activity with an IC₅₀ value of 0.47 ± 0.05 mg/mL. The peptide MDSQ was isolated from the FRPH using electrodialysis with ultrafiltration membrane (EDUF) technology, so it was a potential renin-inhibitory peptide (data not shown).
Specific target protein(s)	Specific Target Protein(s): Renin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Rapeseed protein isolate was subjected to alcalase digestion to obtain a protein hydrolysate.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1411
[D2]	DFBPANHY0033
[D3]	DFBPMUFU0364

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	He R, Girgih AT, Rozoy E, Bazinet L, Ju XR, Aluko RE. Selective separation and concentration of antihypertensive peptides from rapeseed protein hydrolysate by electrodialysis with ultrafiltration membranes. Food Chem. 2016 Apr 15;197(Pt A):1008-14. PMID: 26617047 DOI: 10.1016/j.foodchem.2015.11.081
Other literature(s)	[1] He R , Alashi A , Malomo S A , et al. Antihypertensive and free radical scavenging properties of enzymatic rapeseed protein hydrolysates[J]. Food Chemistry, 2013, 141(1):153-159.
PubDate	2016